Ozyilmaz, G.Ozyilmaz, A. T.Can, F.2024-09-182024-09-1820110003-68381608-3024https://doi.org/10.1134/S0003683811020153https://hdl.handle.net/20.500.12483/13571Amperometric glucose biosensors have been developed based on entrapment on platinum (Pt) electrode using cyclic voltammetry technique in glucose oxidase (GOD) and pyrrole containing p-toluene-sulfonic acid (pTSA) or sodium p-toluenesulfonate (NapTS) as supporting electrolyte solutions. Both of electrolyte solutions were suitable media for the formation and deposition of polypyrrole-GOD (PPy-GOD) layers on Pt substrate. Pt/PPy-GOD electrodes brought about in different morphological properties as well as different electrochemical and biochemical response. The highest responses obtained in pTSA and NapTS electrolytes were observed at pH of 4.5 and 7.0 for Pt/PPy-GOD electrodes, respectively. While linearity was observed between 0.0-1.0 mM glucose substrate for both electrodes, I-max value of Pt/PPy-GOD(NapTS) electrode was approximately twice as high as that of Pt/PPy-GOD(pTSA) electrode as 25.4 and 14.2 mu A, respectively. Five commercial drinks were tested with enzyme electrodes and compared with results obtained spectrophotometrically using glucose kit. Results revealed that Pt/PPy-GODNapTS electrode exhibited better biosensor response.eninfo:eu-repo/semantics/closedAccessHydrogen-PeroxideReducing SugarsBiosensorFilmsPolyanilineAssayElectropolymerizationCoimmobilizationOxidationCatalaseArticle47219620510.1134/S00036838110201532-s2.0-79952399078Q4WOS:000290321000014Q4