Uyar, AhmetDuz, ErkanKeles, Omer F.Yaman, TuranYener, Zabit2024-09-182024-09-1820220372-54801331-8055https://doi.org/10.24099/vet.arhiv.1406https://hdl.handle.net/20.500.12483/12444In this case, a mass located subcutaneously in the perineal subvulvar region of a six-year-old Simmental cow was examined clinically, pathomorphologically and immunohistochemically. Macroscopically, the solitary, whitish yellow-tumor mass was 19x15x6 cm in size, weighed 1610 grams, and had a lobular structure with a few small cystic formations in the section. Histopathological examination revealed that the tumor parenchyma had smooth muscle-like cells with abundant cytoplasm, pleomorphic cells with blunt-ended or cigar-shaped nuclei, anisocytosis, anisokaryosis and karyomegaly. Immunohistochemically, strong positive expression for ??-SMA, vimentin, Ki67 and slight positive for desmin were found, while immulolabeling for pancytokeratin (AE1/AE3), S-100, CD31 and CD34 were negative. In conclusion, on the basis of these findings, the tumor was diagnosed as leiomyosarcoma.eninfo:eu-repo/semantics/openAccesssurgeryhistopathology?-SMAvimentindesminKi67perineal subvulvar leiomyosarcomaSimmental cowArticle92221322110.24099/vet.arhiv.14062-s2.0-85134081382Q4WOS:000798494200011Q4