Ataseven, V. S.Bilge-Dagalp, S.Oguzoglu, T. C.Karapinar, Z.Guzel, M.Tan, M. T.2024-09-182024-09-1820101865-16741865-1682https://doi.org/10.1111/j.1865-1682.2010.01146.xhttps://hdl.handle.net/20.500.12483/9867P>The equid herpesvirus 2 (EHV-2) and 5 (EHV-5), identified agents of respiratory infections and keratoconjunctivitis cases in some equids, comprise a high degree of antigenic heterogeneity. Prevalence and genetic characterization of EHV-2 and EHV-5 strains from Turkey were investigated in this study. A total of 73 nasal swabs and 54 blood specimens were sampled from horses with respiratory tract diseases characterized by mucopurulent nasal discharge and occasional coughing. Overall, EHV-2- and EHV-5-specific DNA amplicons were obtained from 19.2% (14/73) and 21.9% (16/73) of horses tested by multiplex nested PCR. Sequences of EHV-2 and EHV-5 glycoprotein B (gB) gene were used in a phylogenetic analysis that included six EHV-2 and three EHV-5 isolates, which showed that the Turkish EHV-2 and EHV-5 strains have marked sequence divergence from European strains and from each other. Turkish EHV-2 isolates were divided into two distinct subdivisions, and a few isolates were located on a separate branch. This study provides the first epidemiological and phylogenetical report about EHV-2 and EHV-5 infections in Turkey.eninfo:eu-repo/semantics/openAccessequid herpesvirus 2equid herpesvirus 5Mn-PCRrespiratory diseasesequencingTurkeyArticle57427127610.1111/j.1865-1682.2010.01146.x205534262-s2.0-77958137603Q1WOS:000279620600008Q1