Sari, E. KaradagKozlu, T.Kocamis, H.Deprem, T.Asti, R. N.Nazli, M.2024-09-182024-09-1820090035-1555https://hdl.handle.net/20.500.12483/7634The objectives of this study were to investigate the presence of the Alpha Naphthyl Acetate Esterase (ANAE) in peripheral blood leukocytes in goose and to determine the proportions of blood T lymphocytes with this method. Blood smears obtained from 15 geese. 10 month old, were incubated at various pHs (5.0, 5.4, 5.8. 6.2, 6.4. 6.8 and 7.2 for 3 or 6 hours with the enzyme substrate. The highest resolution and consequently the best determination of positive ANAE cells were obtained with pH 5.8 for an incubation period of 6 hours. Positive ANAE activity wits only recorded in monocytes and in some lymphocytes (T cells) according, a diffuse and a granular pattern respectively whereas granulocytes, thrombocytes and B lymphocytes appeared ANAE negative. The mean ratio of ANAE positive lymphocytes peripheral blood ill goose was 53.6 +/- 3.2% and exhibited weak inter-individual fluctuations (coefficient of variations: 5.9%). These results suggest that ANAE staining method could be easily and efficiently used for T cell counting in peripheral blood.eninfo:eu-repo/semantics/closedAccessANAEperipheral blood leukocytesT lymphocytesgooseArticle1604176179WOS:000265869200002Q4