Kazak, F.Cimrin, T.Alasahan, S.Kisacam, M. A.Kutlu, T.2024-09-182024-09-1820231792-2720https://doi.org/10.12681/jhvms.30647https://hdl.handle.net/20.500.12483/7699This study aims to determine the effects of supplementation of different level of black cumin seeds (NS) to quail feeds on carcass characteristics, kidney oxidant antioxidant levels, and ileum histomorphology. A total of 432 mixed-gender three days old Japanese quailswere randomly divided into four groups, including 108 animals each and further into 6 replications consisting of 18 chicks. The groups were fed on either a basal diet alone (Control Group) or a basal diet supplemented with NS at rates of 0.5% (NS-0.5 Group), 1% (NS-1 Group), and 2% (NS-2 Group).It was determined that the back+neck weight in the NS-2 group and head weight and ratio in the NS-1 and NS-2 groups decreased. The thigh rate increased in the NS-0.5 group compared to the control and other NS groups. While the wing weight increased in male quails fed with NS-supplemented feed in NS-1 and NS-2 groups compared to the control group, the abdominal fat weight decreased in NS-supplemented groups.Also, the slaughter weight and hot carcass weight in female quails were significantly reduced in the NS-2 group compared to the control group.Kidney glutathione (GSH), glutathione peroxidase (GPx), and male quails' catalase (CAT) values were increased in NS groups compared to the control group. The kidney vitamin C (Vit C) increased only in the NS-2 group compared to the con-trol. In the NS-2 group, the villus height decreased both on a group basis and in female quails, while the villus width decreased only in female quails. Consequently, it was determined that dietary supplementation of NS at rates of 0.5% increased the thigh rate, dietary supplementation at three different level of NS were effective in maintaining the kidney oxidant-antioxidant balance, and the ileum histomorphology did not change except for dietary supplementation of NS at rates of 2%. Therefore, the result of this study can be stated that NS can be used as a natural antioxidant source in the quail diet.eninfo:eu-repo/semantics/openAccessanimal nutritionantioxidantgenderglutathionehistomorphologyvitamin CArticle7435993600210.12681/jhvms.306472-s2.0-85175012574Q4WOS:001089329300004Q4