Akcora, BulentAltug, Muhammed EnesKontas, TunayAtik, Esin2024-09-182024-09-1820070919-81721442-2042https://doi.org/10.1111/j.1442-2042.2007.01844.xhttps://hdl.handle.net/20.500.12483/9389Aim: Testicular torsion is a serious urological emergency, usually involving newborns, children, and adolescents which can lead to subfertility and infertility. Prevention of testicular damage caused by torsion is still a clinical and experimental problem. So far many chemicals and drugs have been investigated for decreasing ischemia/reperfusion (I/R) injury in experimental animals. The possible protective effect of darbepoetin alfa, a novel erythropoietic protein, on testicular tissue after I/R injury was examined in this study. Methods: Thirty rats were divided into three groups: sham operation, torsion/detorsion, and torsion/detorsion plus darbepoetin alfa groups. After torsion (2 hours) and detorsion (4 hours), bilateral orchiectomy was performed. Malondialdehyde, nitric oxide and glutathione levels were determined in testicular tissue. Results: Administration of darbepoetin alfa caused a decrease of malondialdehyde and nitric oxide levels and an increase in glutathione levels compared with the torsion/detorsion group. In addition, histological injury scores were significantly decreased in the treatment group more than the torsion/detorsion group. Conclusion: The results suggest that darbepoetin alfa may be a potential protective agent for preventing testicular injury caused by testis torsion.eninfo:eu-repo/semantics/closedAccessdarbepoetin alfatestistorsiondetorsionArticle14984685010.1111/j.1442-2042.2007.01844.x177607532-s2.0-34548187170Q1WOS:000249000400013Q4