Ataseven, Veysel S.Ambarcioglu, PinarDogan, Firat2024-09-182024-09-1820232450-73932450-8608https://doi.org/10.2478/jvetres-2023-0010https://hdl.handle.net/20.500.12483/7766Introduction: Bovine viral diarrhoea virus (BVDV) and bovine herpesvirus (BoHV)-1 and -4 are important causes of respiratory diseases and reproductive disorders of dairy cattle worldwide.Material and Methods: Investigation of BVDV and BoHV-1 and -4 antibody levels in the serum and milk of dairy cattle in a group with clinical mastitis and a healthy group was undertaken using an indirect ELISA, and identification of the BoHV-4 genotypes in clinical mastitis cases was attempted by PCR and sequencing.Results: Antibodies specific to BVDV, BoHV-1 and BoHV-4 were detected in the serum and milk of all dairy cattle with clinical mastitis. The cut-off values for BVDV and BoHV-1 in the sera and milk were extremely high in both healthy and mastitic animals. However, BoHV-4 antibodies were detected only in the clinically mastitic cattle, and BoHV-4 levels were higher in milk than in sera among these animals. Genotypes I and II of BoHV-4 were detected in the milk samples of four seropositive cows with clinical mastitis from the same herd.Conclusion: The results of this investigation demonstrate that clinical mastitis cases in the same herd may have aetiology in different BoHV-4 genotypes.eninfo:eu-repo/semantics/openAccessantibodybovine herpesvirus-1bovine herpesvirus-4bovine viral diarrhoea virusmastitisArticle671334010.2478/jvetres-2023-0010370087682-s2.0-85178044934Q2WOS:000950466000001Q2