Sogut, S.Yonden, Z.Kaya, H.Oktar, S.Tutanc, M.Yilmaz, H. R.Yigit, A.2024-09-182024-09-1820111676-5680https://doi.org/10.4238/vol10-2gmr1106https://hdl.handle.net/20.500.12483/12237Oxidative stress may be contributory to the pathophysiology of the abnormalities that underlie the clinical course of sickle cell anemia. We looked for a possible genetic association between the functional polymorphism Ala-9Val in the human Mn-SOD gene and sickle cell anemia. One hundred and twenty-seven patients with sickle cell anemia and 127 healthy controls were recruited into the study. Alanine versus valine polymorphism in the signal peptide of the Mn-SOD gene was evaluated using a primer pair to amplify a 107-bp fragment followed by digestion with the restriction enzyme NgoMIV. In the sickle cell anemia patients, the frequency of Val/Val genotype was approximately 1.4-fold lower and that of Ala/Val was 1.3-fold higher compared to the controls. No significant difference in genotype frequencies was found between patients and controls (chi(2) = 4.561, d.f. = 2, P = 0.101). The Val-9 was the most common allele in patient and healthy subjects. No significant difference in allele frequencies was found between patients and controls (chi(2) = 1.496, d.f. = 1, P = 0.221). We conclude that the Mn-SOD gene polymorphism is not associated with sickle cell anemia.eninfo:eu-repo/semantics/openAccessSickle cell anemiaSuperoxide dismutasePolymorphismOxidative stressArticle10282883310.4238/vol10-2gmr110621574139WOS:000295804800028Q4