Bozkurt, YusufYavas, Ilker2024-09-182024-09-1820140792-156Xhttps://hdl.handle.net/20.500.12483/9269This study examined the effect of two different extenders (carbohydrate and mineral based), supplemented with 10% Dimethyl sulfoxide (DMSO), on cryopreservation of brown trout (Salmo trutta macrostigma) sperm used for hybridization with rainbow trout (Oncorhunchus mykiss). The carbohydrate based extender resulted in higher fertilization (42.5 +/- 1.4%) than the mineral based extender and there was no difference in fertilization rates (p>0.05). This study successfully demonstrated that it is possible to produce Salmo trutta macrostigma x Oncorhynchus mykiss hybrids using cryopreserved sperm from brown trout.eninfo:eu-repo/semantics/closedAccesscryopreservationhybridizationSalmo trutta macrostigmaOncorhynchus mykisstrout hybridsArticle662-s2.0-84899140607Q4WOS:000333534100002Q4