Isik, MuratKisacam, Mehmet Ali2024-09-182024-09-1820240022-32631520-6904https://doi.org/10.1021/acs.joc.3c02864https://hdl.handle.net/20.500.12483/13326We describe SNAr reactions of some commercial amino-tetrazines and halo-dyes, which give efficiently quenched BODIPY/NBD-tetrazines (Phi(Fl) < 0.01) in high yields and, importantly, with high purities affordable via simple silica gel chromatography only. The dyes exhibit large Stokes shifts, moderate environmental sensitivity, and emission enhancements (up to 193-fold) upon Tz ligation with BCN & horbar;a strained dienophile. They successfully serve as labels for HSA protein premodified with BCN, resulting in bright blue-green emission upon ligation.eninfo:eu-repo/semantics/openAccessBioorthogonal ReactionsSmall MoleculesLive CellsProbesCycloadditionDyesDesign1,2,4,5-TetrazinesBicyclononynesDerivativesArticle8996513651910.1021/acs.joc.3c02864385989572-s2.0-85190117842Q2WOS:001201314100001N/A