Sari, E. KaradagKozlu, T.Kocamis, H.Deprem, T.Asti, R.N.Nazli, M.2024-09-192024-09-1920090035-1555https://hdl.handle.net/20.500.12483/14342The objectives of this study were to investigate the presence of the Alpha Naphthyl Acetate Esterase (ANAE) in peripheral blood leukocytes in goose and to determine the proportions of blood T lymphocytes with this method. Blood smears obtained from 15 geese, 10 month old, were incubated at various pHs (5.0, 5.4, 5.8, 6.2, 6.4, 6.8 and 7.2) for 3 or 6 hours with the enzyme substrate. The highest resolution and consequently the best determination of positive ANAE cells were obtained with pH 5.8 for an incubation period of 6 hours. Positive ANAE activity was only recorded in mono-cytes and in some lymphocytes (T cells) according a diffuse and a granular pattern respectively whereas granulocytes, thrombocytes and B lymphocytes appeared ANAE negative. The mean ratio of ANAE positive lymphocytes in peripheral blood in goose was 53.6 ± 3.2% and exhibited weak inter-individual fluctuations (coefficient of variations: 5.9%). These results suggest that ANAE staining method could be easily and efficiently used for T cell counting in peripheral blood.eninfo:eu-repo/semantics/closedAccessANAEGoosePeripheral blood leukocytesT lymphocytesArticle16041761792-s2.0-66349091587N/A