Aygün, OsmanSchneider, ElisabethScheuer, RainerUsleber, EwaldGareis, ManfredMärtlbauer, Erwin2024-09-192024-09-1919990021-8561https://doi.org/10.1021/jf980901fhttps://hdl.handle.net/20.500.12483/14555A competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for histamine in cheese was compared with a reversed-phase liquid chromatography (RP-HPLC) method. Cheese was homogenized with phosphate-buffered saline (PBS), centrifuged, and filtered, and the supernatant was diluted with PBS for CD-ELISA. For RP-HPLC, biogenic amines (histamine, tyramine, putrescine, and cadaverine) were derivatized with 9-fluorenylmethylchloroformate, followed by reversed-phase chromatography and fluorescence detection. Detection limits and mean recoveries (10-1000 mg/kg) were 2 mg/kg and 93% for CD-ELISA and 1 mg/kg and 99% for RP-HPLC, respectively. Analysis of 50 commercial cheeses according to both methods showed good agreement for histamine (r = 0.979; concentration range = 2-1800 mg/kg). At a threshold level of 10 mg/kg, the ELISA gave no false-negative and three false-positive results. The results show that the ELISA is suitable for the determination of histamine in cheese.eninfo:eu-repo/semantics/closedAccessBiogenic aminesCheeseEnzyme immunoassayHistamineLiquid chromatographyArticle4751961196410.1021/jf980901f105524782-s2.0-0032844008Q1