Yavas, IlkerBozkurt, YusufYildiz, Cengiz2024-09-182024-09-1820140967-61201573-143Xhttps://doi.org/10.1007/s10499-013-9698-6https://hdl.handle.net/20.500.12483/12127The aim of the present study was to determine the effect of various cryoprotectants on post-thaw sperm quality and fertilizing capacity of cryopreserved scaly carp (Cyprinus carpio) semen. The present study focused on freezing of scaly carp sperm utilizing a practical and inexpensive protocol for aquaculture. Semen was diluted with Kurokura's extender composing 3.6 g/l NaCl, 10 g/l KCl, 0.22 g/l CaCl2, 0.08 g/l MgCl2 and 0.2 g/l NaHCO3. The extender contained three different cryoprotectants (DMSO, DMA and egg yolk) at ratios of 5, 10 and 15 %. Semen was placed into 0.25-ml straws and exposed to liquid nitrogen vapor (-120 A degrees C) using an insulated box with an adjustable tray for 10 min and then plunged into liquid nitrogen (-196 A degrees C) tank. The thawing process was performed in a water bath at 40 A degrees C for 10 s. The results indicated that type of cryoprotectants and their concentrations are rather effective in scaly carp sperm cryopreservation on post-thaw sperm quality, while they are very important in order to obtain high fertilization rates. The highest fertilization rate was determined as 96.4 +/- A 0.15 % with 15 % egg yolk, while the highest hatching rate was determined as 99.3 +/- A 0.80 with 15 % DMA. In conclusion, the applied cryopreservation method for scaly carp sperm is suitable to fertilize high amounts of eggs.eninfo:eu-repo/semantics/closedAccessScaly carpCyprinus carpioCryoprotectantCryopreservationMotilityFertilizationHatchingArticle22114114810.1007/s10499-013-9698-62-s2.0-84892433105Q1WOS:000329939600014Q3