Boztas, KadirKocabag, Hamide DenizDerecik, KayhanGazel, MonaSipahioglu, Hikmet MuratCaglayan, KadriyeTulum, Isil2024-09-192024-09-1920232249-4669https://doi.org/10.5958/2249-4677.2023.00057.9https://hdl.handle.net/20.500.12483/14188In the winter 2019 virescence, flower sterility, and stunting symptoms were observed in daffodil plants in Mersin province of Turkey. To detect the presence of phytoplasmas ten plants showing symptoms and ten asymptomatic plants were assayed by PCR analysis. The nested-PCR analysis of extracted DNA with universal primer pairs amplifying 16S phytoplasma ribosomal gene were positive for 9 out of the 10 symptomatic daffodil samples. BLAST analysis of the 16S rDNA sequences, virtual restriction fragment length polymorphism (RFLP) and RFLP analyses confirmed the presence of ‘Candidatus Phytoplasma mali’ in Narcissus tazetta plants. The detected strains of ‘Ca. P. mali’ were sequenced and compared in multiple alignments using sequences in NCBI GenBank. The nucleotide identity among the 16S rDNA gene fragments ranged between 99.26 and 99.91% to ‘Ca. P. mali’, ribosomal subgroup 16SrX-A. Although phytoplasmas in this subgroup have been reported frequently from woody plants, this is the first report of a ‘Ca. P. mali’ strain infecting daffodil plants. © 2023, Technology Society of Basic and Applied Sciences. All rights reserved.eninfo:eu-repo/semantics/closedAccess16SrX-A subgroupdaffodilPCR/RFLPphyllodyArticle13111311410.5958/2249-4677.2023.00057.92-s2.0-85163590442Q4