Kısaçam, Mehmet AliOğlu, Gonca Ozan Kocamu FtuOzan, EnverOzan, Penbe Sema Temı Zer2024-09-192024-09-1920221309-69582146-1953https://doi.org/10.15312/EurasianJVetSci.2022.386https://search.trdizin.gov.tr/tr/yayin/detay/1170362https://hdl.handle.net/20.500.12483/15553Aim: Reactive oxygen species (ROS) are produced during the metabolism of diethylnitrosamine (DEN) and phenobarbital (PB). Ellagic acid (EA) is a natural biologically active polyphenol with antioxidant activity. The present study was conducted to assess the preventive and protective capability of EA on DEN-PB-induced oxidative damage in rats. Materials and Methods: 60 Sprague-Dawley rats were assigned into 6 groups: Negative control, DEN, EA, DEN-PB (positive control), DEN-PB-EA (treatment), and EA-DEN-PB (pretreatment). 150 mg/kg DEN were administered to DEN, positive control, treatment and pretreatment groups. After two weeks, 500 ppm PB applied to positive control and treatment groups. 50 mg/kg/day EA were orally given to EA, and pretreatment groups for 8 weeks and treatment group was given for four weeks. After 8 weeks rats were sacrificed, liver and blood were subjected to biochemical evaluation. Results: DEN alone or with PB increased liver MDA levels significantly; however plasma MDA levels didn’t change. EA pretreatment decreased liver MDA levels (p<0.001). CAT activity did not change in the liver and erythrocytes of the positive control group. Liver CAT activity increased insignificantly in pretreatment and significantly in treatment group (p<0.05). SOD activity did not change in liver and erythrocytes of DEN and positive control groups. EA increased liver SOD activity significantly (p<0.001). DEN application alone or with PB decreased liver and blood GSH levels significantly (p<0.05), while EA application increased liver GSH levels in treatment (p<0.05). Blood GSH levels decreased significantly in treatment (p<0.001). Conclusion: It is considered that EA pretreatment could be more effective than EA treatment in DEN, PB induced oxidative stress in rats.eninfo:eu-repo/semantics/openAccessArticle38422523110.15312/EurasianJVetSci.2022.3861170362