Özkan, HüseyinDalkıran, SevdaKaraaslan, İremKaya, UfukAlakuş, İbrahimÇamdeviren, BaranYakan, Akın2024-09-192024-09-1920221304-72802267-5498https://doi.org/10.32707/ercivet.1142609https://search.trdizin.gov.tr/tr/yayin/detay/1107107https://hdl.handle.net/20.500.12483/16029This study aimed to investigate the effects of food restriction on oxidative status at the molecular levels of the testis in rats. A total of 16 male Wistar rats were allocated to two groups (n=8) and fed for four weeks. The first group was control (Cont) and consumed food and water ad libitum. The second one, food-restricted group (FR) was presented half of the regular consumption. But the water was presented ad libitum. The feeding period was maintained for four weeks. At the end of the feeding period, rats were euthanized by cardiac blood sampling under anesthesia. Malondialdehyde (MDA) levels in testis tissue were determined and calculated as nmol/mg protein. In addition, Prosta-glandin-Endoperoxide Synthase 2 (PTGS2) and Nuclear factor erythroid 2–related factor 2 (NRF2) genes expression levels were determined in testis tissue. While MDA and gene expression levels were found similar in groups, a positive correlation was found between PTGS2 and NRF2 genes (r=0.629; P<0.05). The molecular regulation of oxidative sta-tus was found strongly related with PTGS2 and NRF2 genes molecular activity in testis of rats. The obtained results were shown that the feeding period and restriction rate factors were mainly responsible for the oxidative status of testis tissue.eninfo:eu-repo/semantics/openAccessOxidative stressPTGS2testisFood restrictionNRF2Article19212312810.32707/ercivet.11426091107107