Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination

Abstract

The possibility of obtaining dihaploid durum wheat ( Triticum durum L.) plants by interspecifc hybridization with Imperata cylindrica L. was studied. Ten local varieties and 1 commercial durum wheat variety (Triticum durum L., 2n = 4x = 28; AABB) were used as maternal lines. Imperata cylindrica L. (2n = 2x = 20) ecotypes sampled from diferent districts of Hatay were used as the pollen source. Isolated embryos were placed in an MS medium for plant regeneration 14 days afer the interspecifc hybridization. Te highest average seed setting, embryo formation, and dihaploid plant regeneration for the diferent hybrid combinations were determined as 31.1%, 15.5%, and 11.5%, respectively. Cytological observations revealed that all the regenerants had 2n = 2x = 14 chromosomes.

The possibility of obtaining dihaploid durum wheat ( Triticum durum L.) plants by interspecifc hybridization with Imperata cylindrica L. was studied. Ten local varieties and 1 commercial durum wheat variety (Triticum durum L., 2n = 4x = 28; AABB) were used as maternal lines. Imperata cylindrica L. (2n = 2x = 20) ecotypes sampled from diferent districts of Hatay were used as the pollen source. Isolated embryos were placed in an MS medium for plant regeneration 14 days afer the interspecifc hybridization. Te highest average seed setting, embryo formation, and dihaploid plant regeneration for the diferent hybrid combinations were determined as 31.1%, 15.5%, and 11.5%, respectively. Cytological observations revealed that all the regenerants had 2n = 2x = 14 chromosomes.