Determination of the seroprevalence of Leptospirosis in cattle by MAT and ELISA in Hatay, Turkey

Abstract

Hatay ili merkez köy ve ilçelerinde Leptospirosis'in seroprevalansım belirlemek amacıyla Nisan-Temmuz 2003 aylarında tesadüfi örnekleme ile değişik yaş ve cinsiyette toplam 512 adet sığırdan alınan kan örnekleri 3 farklı Leptospira interrogans serovarina (grippotyphosa, hardjo ve icterohaemorrhagie) karşı oluşan antikorlar yönünden mikroskopik aglutinasyon testi (MAT) and enzyme-linked immunosorbent assay (ELISA) ile incelendi, incelenen serum örneklerinin 45'inde (% 8,8) MAT, 72'sinde (% 14) ELISA ile pozitiflik saptandı. MAT ile pozitif bulunan serum örneklerinin 26'sı (% 57,7) serovar grippotyphosa, 9'u (% 20) serovar hardjo, 1 'i (% 0,2) serovar icterohaemorrhagie ve 9'u (% 20) da serovar grippotyphosa + serovar hardjo ile pozitif reaksiyon verdi. ELISA ile pozitif bulunan serum örneklerinin ise 32'si (% 44,4) serovar grippotyphosa, 15'i (% 20,8) serovar hardjo, 2'si (% 2,8) serovar icterohaemorrhagie ve 23'ü de (% 31,9) serovar grippotyphosa + serovar hardjo ile pozitif reaksiyon verdi. Dominant serovar olarak grippotyphosa belirlendi. Yerleşim yerleri arasında seroprevalans değerleri istatistiksel olarak önemli farklılık gösterirken (P < 0,05), yaş ve cinsiyetin pozitiflik oranları üzerine önemli bir etkisi görülmedi (P > 0,05).

Five hundred and twelve serum samples collected from randomly selected cattle of different age and sex from the towns and central villages of Hatay during April-July 2003 were tested for antibodies against 3 different Leptospira interrogans serovars {grippotyphosa, hardjo and icterohaemorrhagie) using a microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA). Forty-five (8.8%) and 72 (14%) of the 512 serum samples tested were detected to be positive with MAT and ELISA, respectively. 0f the 45 MAT positive serum samples, 26 (57.7%) were positive against serovar grippotyphosa, 9 (20%) were positive against serovar hardjo, 1 (0.2%) was positive against serovar icterohaemorrhagie and 9 (20%) were positive against serovar grippotyphosa + serovar hardjo. Of the 72 ELISA positive serum samples, 32 (44.4%) were positive against serovar grippotyphosa, 15 (20.8%) were positive against serovar hardjo. 2 (2.8%) were positive against serovar icterohaemorrhagie and 23 (31.9%) were positive against serovar grippotyphosa + serovar hardjo. The dominant serovar was grippotyphosa. Statistical analyses indicated a significant difference in seroprevalence among locations (P &lt; 0.05); however, no significant differences in positivity rates were found with respect to the age or sex of the animals (P &gt; 0.05).