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Öğe Begonia semperflorens'in direk organogenesis yöntemiyle rejenerasyonu(2008) Yalçın Mendi, Neslihan Yeşim; Kocaman, Esra; Ünek, Ceren; Eldoğan, Selay; Çürük, Pembe; Gencel, Günsu; Serçe, SedatThe plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, MS media containing different concentrations of BA (0.0, 0.2, 0.5, 1.0 mg/l.) with NAA (0.0, 0.2, 0.5 mg/l.) were investigated to optimize regeneration of Begonia semperflorens. The best regeneration and growth were obtained from the medium containing 0.5 BA - 0.5 NAA (67.5%) followed by 0.5 BA - 0.2 NAA (66.7%) and 0.2 BA - 0.5 NAA (65.0%) in BA and NAA combination.