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    Öğe
    In vitro regeneration of some Turkish melon (Cucumis melo L.) cultivars
    (Diagnosis Press Ltd, 2002) Çürük, S; Çetiner, S; Gaba, V
    Regeneration from cotyledon explants of Turkish melon (Cucumis melo var. inodorus and var. reticulatus) cvs. Hasanbey I, Yuva, Kirkagac 637, Topatan, Kuscular and Ananas were investigated. Two regeneration media were used: IK1560 (Moreno et al., Plant Cell, Tissue Organ Cult. 5, 139, 1985). and the regeneration medium of Neidz et al., Plant Cell, Tissue Organ Cult., 18, 313, 1989 (N medium). In vitro cultures were placed in a growth room at 29+/-1degreesC, with 16 h photoperiod and 100-120 mumol m(-2) s(-1) cool white fluorescent light. Regeneration on N medium was higher than on IK1560. Greater regeneration was obtained from explants of 4 and 5-day-old cotyledons than from explants from 6-day-old cotyledons. Kirkagac 637 showed the highest regeneration rate of all the cultivars. Green regenerable callus formation was better on IK1560 medium than on N medium. The cv. Topatan had a higher green callus formation rate than the other cvs. Moreover, the explants originating from N regeneration medium produced more buds and/or shoots on the shoot elongation medium NB00101 (Vallis and Lasa, Plant Cell Rep. 13, 145, 1994) than those from IK1560 regeneration medium.
  • Küçük Resim
    Öğe
    Transformation of recalcitrant melon (Cucumis melo L.) cultivars is facilitated by wounding with carborundum
    (Wiley-Blackwell, 2005) Çürük, S; Çetiner, S; Elman, C; Xia, X; Wang, Y; Yeheskel, A; Zilberstein, L
    Transformation of the recalcitrant melon (Cucumis melo L.) cultivars Kirkagac 637 and Noi Yarok was accomplished by wounding cotyledon explants by vortexing with carborundum prior to inoculation with Agrobacterium tumefaciens. The addition of silver nitrate to the regeneration-selection medium reduced the transformation efficiency, as the percentage of the explants forming putative transgenic calli and bud-like protuberances was decreased and no transgenic shoots were produced. Chimeric transgenic plants were obtained after the regeneration of putatively transformed callus, bud-like protuberances, buds and shoots on selective medium with kanamycin. The treatments producing the most buds or shoots from explants after 30-40 days of cultivation were the most successful for the production of transgenic plants. Only treatments where explants were vortexed with carborundum produced transgenic melon shoots of either cultivar. Subculture every 18-20 days on fresh regeneration-selection medium containing 50 mg/L kanamycin after either a relatively high (100 mg/L) or low level (50 mg/ L) of kanamycin in the first regeneration-selection medium was necessary for the successful transformation of cultivar Kirkagaq 637. These techniques are now being used in breeding programs for the production of melon lines bearing resistances to zucchini yellow mosaic virus and cucumber mosaic virus, important viruses limiting agricultural production.