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    Embryo Transfer in Fleckvieh Simmental Cows: A Preliminary Study
    (Unique Scientific Publishers, 2022) Köse, Ayşe Merve; Çizmeci, Sakine Ülküm; Dinç, Dursun Ali
    This study was aimed at investigating pregnancy rates achieved with multiple ovulation and embryo transfer in Simmental cows, which are characterized by a high milk yield. For this purpose, eight cows were used as donors and for each donor, recipient groups of 10 animals, including both heifers and cows, were established. Recipients were synchronized by two intramuscular injections of prostaglandin F2? 11 days apart. The treatment of the donors consisted of the intravaginal placing of a CIDR on day 0, the application of 400mg FSH in decreasing doses, starting on day four, the administration of prostaglandin F2? and the removal of CIDR on day 8. The donors were inseminated twice on day 9 and once on day 10. Uterine flushing was performed, trans-cervically, on day 7 after insemination. The ovulation rate was determined as 12.25±1.42. Sixteen transferable embryos (2.0±3.23, 88.9%) were collected. The 12 embryos of Grade 1 quality and the 4 embryos of Grade 2 quality were transferred by transcervical route to 12 heifers and 4 cows, respectively. The pregnancy rates achieved in the heifers and cows were 50% and 25%, respectively (P>0.05). As a result, the MOET scheme applied in Fleckvieh Simmental Cows produced satisfactory superovulatory responses and resulted in the collection of quality embryos. The embryo recovery rate was poor but, overall, the ovulation rate, the quality of the collected embryos and the pregnancy rates achieved under field conditions in this preliminary study were considered to be satisfactory. © 2022, Unique Scientific Publishers. All rights reserved.
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    Investigation of the effect of a progesterone free superovulation protocol on embryo yield and quality in goats
    (Mehria Publishers, 2017) Çizmeci, Sakine Ülküm; Köse, Ayşe Merve; Güler, Mehmet; Tekeli, Tevfik
    The main objective of the present study is to determine the effects of progesterone and progesterone-free superovulation protocols on embryo count and quality in goats. The animal material of the study is consisted of 1-3-year-old 22 goats and 3 bucks. During the breeding season, the goats were divided into two groups of 11, and superovulation was applied. In the control group, goats were synchronized with intravaginal sponges containing 20 mg of fluorogestone acetate for 12 days. Four days after oestrus in the experimental group and on the 9th day after sponge application in control group decreasing doses of FSH (Follicle stimulating hormone) (50; 30; 20 mg) were injected intramuscularly at 12 hour intervals. The sponge was removed on the 12th day in the control group. In both groups, oestrus was observed 24 hours after the fifth dose of FSH and the uterus flushing was performed on the 7th day after mating. In the study, superovulation response was obtained 100% in control group and 25% in experimental group. The recovery rate and transferable embryos were determined 61.5; 82.81 and 23.3%; 71.43% respectively and there was no difference between the groups (P > 0.05). In the non-progesterone-applied group of animals, it was observed that the cycle cannot be fully dominated, sufficient progesterone level could not be reached and gonadotropin application was, therefore, insufficient. Although progesterone administration is thought to have a negative effect on embryo quality, applying a superovulation by using progesterone has been shown to be more successful on superovulation and embryo collection rates. © 2017 IJVS.