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Öğe Comparative efficacy of three anesthetic agents on juvenile African catfish, Clarias gariepinus (Burchell, 1822)(2013) Öğretmen, Fatih; Gökçek, KayaBu çalışmada, üç anestezik maddenin (karanfil yağı, fenoksiethanol ve eugenol) etkinliği kültür şartları altında yetiştirilmiş Karabalık’larda karşılaştırılmıştır. Etkin en düşük doz kriteri olan 180 s içinde bayılma ve 300 s içinde ayılma hali karanfil yağında 50 mg L-1 (bayılma 193±62 s ve ayılma 251±32 s), fenoksiethanolde 750 ?l L-1 (bayılma 145±24 s ve ayılma 174±10 s) ve eugenolde 50 mg L-1 (bayılma 197±29 s ve ayılma 310±17 s) dozlarında elde edilmiştir. Bireysel olarak bayılma ve ayılma hallerinin başlangıcı, kullanılan anestezik maddenin dozuna bağlı olarak istatistiki açıdan farklı bulunmuştur (P<0,05). Anestezik madde konsantrasyonu ile bayılma süresi arasında ters bir üssel ilişki tespit edilmiş, ancak anestezik madde konsantrasyonu ile ayılma süreleri arasındaki ilişki üssel olarak ölçülmüştür. Bu çalışmanın sonucunda, kullanılan üç anestezik maddeden Karabalıklar için en uygun olanın karanfil yağı olduğu söylenebilir.Öğe Effect of different extenders and storage periods on motility and fertilization success of grass carp (Ctenopharyngodon idella) Sperm during spawning season(2009) Bozkurt, Yusuf; Öğretmen, Fatih; Seçer, Faik SertelThe present study was conducted to evaluate the effect of different extenders and storage periods on motility and fertilizing ability of short-term stored grass carp sperm during spawning season. Sperm was collected from anesthesized males by the abdominal massage method. Having determined the main spermatological properties, the pooled ejaculates were diluted with 3 different extenders at a ratio of 1:3. The diluted sperm was packaged in small tubes and following, the tubes were stored at 4°C in a refrigerator for 8 hours. During preservation motility (%), motility duration (s) and fertilization (%) capacity of stored sperm were evaluated 2 hours intervals. Fertilization was carried out using the dry fertilization technique at 2x105 spermatozoa/egg dosage. The fertilization success was determined as the percent of eyed eggs 3-4 days after fertilization. At the end of 8 hours of storage, the highest sperm motility was found as 35±2.89% (P<0.05) with extender II in June whereas the highest motility duration was determined (56±6.35 s) (P<0.05) with extender I in May. Also, the highest fertilization rate was determined as 25±1.15% (P<0.05) with extender I in June. In conclusion, results of the present study indicated that composition of extenders, spermatozoa/egg ratio and stage of the spawning season is important to improve fertilizing ability of cold stored grass carp sperm.