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  • Küçük Resim
    Öğe
    Investigation of antiseptic resistance genes in Staphylococcus spp. isolates
    (Natural and Engineering Science, 2020) Akin, Mustafa; Özcan, Birgül; Cantekin, Zafer; Ergün, Yaşar; Bulanık, Dilşad
    Antiseptic and disinfectants are used very frequently in all health institutions, including hospital and veterinary application areas, in the home environment and food production industry, to prevent infections and contaminations. At present, the quaternary ammonium compounds, benzalkonium chloride and chlorhexidine digluconate is one of the divalent cations most commonly used chemicals such as antiseptics and disinfectants. However, the widespread use of biocides has brought about the emergence of bacteria resistant to antiseptics/disinfectants. It is known that bacteria develop resistance mechanisms against antibiotics as well as disinfectants. Epidemiological data on antiseptic susceptibility and distribution of resistance genes are very important for nosocomial infections. Some species, including the species belong to genus Staphylococcus, cause foodborne poisoning and various clinical infections such as skin and soft tissue and surgical site infections, endocarditis, mastitis, pneumonia and bacteremia in humans and animals. Staphylococcus strains can contain plasmid-derived qacA/B and qacC genes that provide resistance to quaternary ammonium compounds (QAC). In this study, the presence of antiseptic resistance genes (qacA/B and qacC) in 90 Staphylococcus spp. strains isolated from chicken carcass, bovine tank milk, various cheeses and bovine clinical mastitis samples were determined by simplex polymerase chain reaction. QacA/B was found in %18.8 and qacC in %2.2 of the studied isolates. Of antiseptic resistance genes, qacA/B was detected in cheese and bovine clinical mastitis samples, and qacC in chicken carcass. © 2020, Natural and Engineering Science. All rights reserved.
  • Küçük Resim
    Öğe
    Zeytinyağı fabrikası atığında üretilen Lentinus tigrinus ve Laetiporus sulphureus funguslarında kültür periyoduna bağlı olarak gibberelik asit (GA3) absisik asit (ABA) ve sitokinin (Zeatin) üretimi
    (Hatay Mustafa Kemal Üniversitesi, 1997) Özcan, Birgül; Topcuoğlu, Fatih
    Bu çalışmada, statik inkübasyon ortamında ve zeytinyağı fabrikası atığı besiyerinde üretilen beyaz-çürükçül fungus Lentimus ügrimus ve kahverengi-çürükçül fungus l.aetiponıs sufphureus,\m kültür ortamında kültür periyoduna bağlı olarak serbest-, bağlı- ve toplam- formlarda içsel gibberellik asit (GA3), absisik asit (ABA) ve sitokinin (zeatin) miktarları ve fungusların kuru misel ağırlıkları saptanmıştır. İçsel- GA?, -ABA ve -zeatin miktarlarının tayininde spektrofotometre tekniği kullanılmıştır. Çalışmamızın sonucunda çalışmada kullanılan fungusların primer ya da sekonder bir metabolit olarak GA3, ABA ve zeatin sentezledikleri ve sentezlenen bu bitkisel hormonların hem serbest ve hem de bağlı formlarda bulunduğu gösterilmiştir.