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  • Küçük Resim
    Öğe
    Begonia semperflorens'in direk organogenesis yöntemiyle rejenerasyonu
    (2008) Yalçın Mendi, Neslihan Yeşim; Kocaman, Esra; Ünek, Ceren; Eldoğan, Selay; Çürük, Pembe; Gencel, Günsu; Serçe, Sedat
    The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, MS media containing different concentrations of BA (0.0, 0.2, 0.5, 1.0 mg/l.) with NAA (0.0, 0.2, 0.5 mg/l.) were investigated to optimize regeneration of Begonia semperflorens. The best regeneration and growth were obtained from the medium containing 0.5 BA - 0.5 NAA (67.5%) followed by 0.5 BA - 0.2 NAA (66.7%) and 0.2 BA - 0.5 NAA (65.0%) in BA and NAA combination.
  • Küçük Resim
    Öğe
    Determination of gene escape and fruit quality characteristics in transgenic melon (Cucumis melo L. var. inodorus)
    (2010) Yalçın Mendi, Yeşim; Sarı, Nebahat; Akyıldız, Asiye; Solmaz, İlknur; Ünek, Ceren; Özkaya, Okan; Serçe, Sedat
    Gene escape and fruit quality characteristics of transgenic melons (Cucumis melo L. var inodorus cv. ‘Kirkagac 637’) resistant to zucchini yellow mosaic virus (ZYMV) and control plants were investigated under screenhouse conditions. No significant differences were observed between transgenic and transgenic × control genotypes, with regard to rind thickness, fruit cavity length, fruit cavity width, total soluble solids, pistil scar diameter, and peduncle length. Fruit characters, including fruit weight, fruit width, fruit length, fruit flesh thickness, and peduncle diameter were significantly different. These results indicate that transgenic × control genotypes had higher values than transgenic (T4 and T20) genotypes, regarding fruit weight, fruit width, fruit length, fruit flesh thickness, and peduncle diameter. Significant differences were not observed between transgenic (T4 and T20), control, and transgenic × control genotypes in terms of L-ascorbic acid, malic acid, citric acid, sucrose, glucose, or fructose, but differences were observed for fruit total acidity. Esters, lactones, and alcohols were aroma components, but none differed significantly between the transgenic and control genotypes. The results show that there was 100% gene escape in the control plants within 10 m of the transgenic plants, while there was 70% gene escape in plants 12.5, 15, and 17.5 m from the transgenic plants under screenhouse conditions.
  • [ N/A ]
    Öğe
    Transgenik Kırkağaç 637 kavun çeşidinde morfolojik karakterizasyon
    (2007) Yalçın Mendi , Yeşim; Sarı, Nebahat; Solmaz, İlknur; Ünek, Ceren; Eldoğan, Selay; İpek, Muzaffer; Serçe, Sedat
    Bu çalışmada, Kabak Sarı Mozayik Virüsüne (ZYMV) dayanıklı olduğu varsayılan transgenik Kırkağaç 637 kavun çeşidinin ve kontrol bitkilerinin screenhouse koşullarında bitki özellikleri incelenmiştir. Değerlendirmeye alınan parametreler fide aşamasında kotiledon eni, kotiledon boyu ve hipokotil uzunluğu; bitki aşamasında ise bitki boyu, ana gövde çapı, boğum sayısı, yaprak sapı uzunluğu, yaprak genişliği ve yaprak boyudur. Yapılan analizler sonucunda transgenik bitkilerde kotiledon eni ve hipokotil uzunluğunun, transgenik olmayan bitkilere göre daha kısa olduğu bulunmuş, kotiledon boyları bakımından ise istatistiksel olarak bir fark saptanmamıştır. Transgenik ve transgenik olmayan bitkiler arasındaki bitki boyu (%1 önem seviyesinde), ana gövde çapı, boğum sayısı ve yaprak sapı uzunluğu da (%5 önem seviyesinde) istatistiksel olarak farklı bulunmuştur.