Yazar "Şevik, Murat" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    Epidemiological investigation of Crimean-Congo hemorrhagic fever infection in cattle in some provinces of Turkey
    (2018) Şevik, Murat
    Crimean-Congo hemorrhagic fever (CCHF) is a zoonotic disease which causes lethal hemorrhagic fever in humans. The purpose of this study was to investigate the CCHF infection status in cattle in the Central Anatolia region and the central-west part of the Aegean region of Turkey. For this purpose, EDTA whole blood (n = 329) and sera (n =329) samples were obtained from epidemiologically independent herds (n =94) during the months of March 2016 and September 2017. The exposure status to CCHF was determined using ELISA for detection of CCHF virus (CCHFV) specific IgG antibodies in cattle sera samples. Real-time reverse-transcriptase PCR was used to detect viral RNA in EDTA whole blood samples. The CCHFV-specific IgG antibodies were detected in 4 out of 329 animals, accounting for 1.2% prevalence rate. CCHFV RNA was not detected in EDTA whole blood samples. The low seroprevalence suggests only sporadic introduction of CCHFV. Further epidemiological studies are needed to determine the distribution of CCHFV infection in livestock in Turkey.
  • Küçük Resim
    Öğe
    Molecular Characterization of Partial RdRp Genes of Tomato Ringspot Virus Isolates from Turkey
    (2021) Akdura, Nevin; Şevik, Murat
    Tomato ringspot virus (ToRSV) is a virus having isometric structure and single helix positive sense and is formed by a combination of two genomic RNA (RNA-1 and RNA-2) in the Secoviridae family from the Nepovirus group, and it causes economically devastating diseases in a wide range of hosts. In this study, 300 leaf samples from tomato (Solanum lycopersicum), pepper (Capsicum annuum), cucumber (Cucumis sativus) and grapevine (Vitis vinifera) plants exhibiting virus and virus-like symptoms were collected from three different districts of Hakkari province between September 2014 and July 2015. The samples were tested for ToRSV infection on DNA fragments of 411 bp in size by Reverse Transcription Polymerase Chain Reaction (RT-PCR), using specific primers for the RNA dependent RNA polymerase (RdRp) gene. Seven out of 300 leaf samples (2.3%) were found to be infected with ToRSV. The virus was detected in tomato, pepper and cucumber samples, but its presence was not so evident in grapevine plants. The DNA sequences were submitted to GenBank (Accession numbers; KT728407, KT728408, KT728409 and KT728410). Our ToRSV isolates were compared with the ones available in GenBank, and the analysis of four ToRSV isolate RdRp gene sequences revealed that the nucleotide and amino acid homology were 83-100% and 94 100%, respectively. Phylogenetic analysis suggested that Turkish ToRSV isolates and currently available ToRSV isolates in GenBank were divided into three distinct groups. This analysis provides, to the best of our knowledge, the first report on the partial RdRp sequence of ToRSV in Turkey.