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  • Küçük Resim
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    The effect of mycorrhiza in nutrient uptake and biomass of cherry rootstocks during acclimatization
    (Ars Docendi, 2010) Aka-Kacar, Yildiz; Akpinar, Cagdas; Agar, Aslihan; Yalcin-Mendi, Yesim; Serce, Sedat; Ortas, Ibrahim
    The effect of arbuscular mycorrhizal fungi (AMF) on growth and nutrient uptake of micropropagated cherry rootstocks was evaluated during acclimatization and plant establishment. Two commonly used cherry rootstocks, 'Edabriz' and 'Gisela 5', were propagated through tissue culture and grown in a greenhouse for 16 weeks. Plantlets were inoculated with Glomus Glomus caledonium, Glomus etunicatum. Glomus intraradices, Glomus mosseae, cocktail (mixture of these species) and indigenous mycorrhiza into three different substrate mixtures. All micropropagated cherry plantlets survived transplanting. After 16 weeks. mycorrhizal plantlets had greater nutrient uptake than non-mycorrhizal plantlets. Roots of inoculated cherry plantlets were heavily colonized with AMF. These results indicated that mycorrhizal inoculation during transplantation from in vitro to ex vitro culture can induce growth responses. The experiments also showed that the mycorrhizal cherry rootstocks were healthier and had higher Zn and P contents when compared to controls for both rootstocks. G. mosseae was one of the most efficient AMF species. Indigenous AMF isolated from Cukurova region also significantly increased the plant growth and nutrient uptake. 'Gisela 5' rootstocks had significantly higher P and Zn contents than 'Edabriz'. Taken together, our results indicate that AMF inoculations enhance growth and development of micropropagated plants which would be beneficial to improve cherry rootstock production.
  • Küçük Resim
    Öğe
    The Effects of Different Hormones on Regeneration of Gazania (Gazania rigens)
    (Ars Docendi, 2009) Yalcin-Mendi, Yesim; Unek, Ceren; Eldogan, Selay; Aka-Kacar, Yildiz; Serce, Sedat; Curuk, Pembe; Kocaman, Esra
    Recent advances in the modern techniques of propagation could help growers to meet the demand of the ornamental industry. New ornamental plant varieties are being created by breeders in response to consumer demand for new products. In this study, different concentrations of BA (0.0, 0.2, 0.5, 1.0 mg/l) and IAA (0.0, 0.5, 1.0 mg/l) were investigated to optimize regeneration of Gazania rigens. The best callus formation was found from the media containing 0.2 mg/l BA-1.0 mg/l IAA (100%), 0.5 mg/l BA-1.0 mg/l IAA (98%), 0.5 mg/l BA-0.5 mg/l IAA (97%), 1 mg/l BA -1 mg/l IAA (97%), 1.0 mg/l BA-0.5 mg/l IAA (98%). 0.5 mg/l BA (97%). The most promising regeneration and growth were obtained from the media with 0.2 mg/l BA-1.0 mg/l IAA (30%). 1.0 mg/l BA-0.5 mg/l IAA (22%) and 1.0 mg/l BA (18%). The regenerated plantlets were rooted on the media containing 0.5 and 1.0 mg/l IAA. The best rooting percentage was observed on the media containing 1.0 mg/l IAA (75%).
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    Öğe
    Relationships among myrtle accessions from Turkey as revealed by fruit characteristics and RAPD
    (Ars Docendi, 2008) Serce, Sedat; Simsek, Ozhan; Gunduz, Kazim; Aka-Kacar, Yildiz; Ercisli, Sezai
    Myrtle, Myrtus communis L., is one of the most important and widespread shrubs (maquis) throughout the Mediterranean region. Its fruits ripen during the period of mid-fall to early winter and are generally dark-colored although while-colored wild forms rarely exist. In Turkey, both forms are present in addition to a while and large-fruited type, cultivated in small orchards and marketed locally. We studied two sets of myrtle accessions from Hatay, Turkey. Set I consisted of two wild accessions, dark- and white-colored forms, and six cultivated selections, while Set 2 contained three open-pollinated individuals from each accession of Set 1. We evaluated fruit characteristics of including fruit weight, width, length, soluble solids, pH and acidity. The accessions considerably differed in all the traits tested except for soluble solids. We also examined genotypic variation of Sets I and 2 using 26 RAPD primers and analyzed the data by cluster and principle components analyses. The results revealed that although some of the half-sib families (e.g., 8 a, b and c) were grouped based on their maternal parents, no overall pattern was apparent. These results suggest that myrtle pollination appeared to be not restricted to the self-pollination and the cultivated types have a narrow genetic base.