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Öğe A Comparative Study of Seminal Plasma and Blood Serum Macro and Trace Elements in the the Breeding (October) and the Non-Breeding (April) Seasons in Merino Ram(Hellenic Veterinary Medical Soc, 2019) Bulbul, B.; Akalin, P. P.; Baspinar, N.; Bucak, M. N.; Kirbas, M.; Ozturk, C.; Gungor, S.In this study, it was aimed to investigate the concentrations of macro and trace elements in seminal plasma and blood serum in the breeding (October) and the non-breeding (April) seasons in Merino Ram. Nineteen Merino Rams, aged 18-24 months, were involved in the study. Blood (once) and ejaculate samples (6 replicates) were taken in the breeding (October) and the non-breeding (April) seasons. Blood serum, seminal plasma and diet Calcium, Sodium, Potassium, Magnesium, Phosphorus, Sulfur, Zinc, Selenium, Chrome, Manganese, Nickel, Molybdenum and Boron concentrations were determined by ICP-AES. In blood serum, Sodium and Selenium concentrations were higher (p<0.05 and 0.001, respectively) in the the breeding season than in non-breeding season, whereas Potassium, Chromium and Boron concentrations were lower (p<0.05, 0.001 and 0.001, respectively) in the breeding season than in the non-breeding season. In seminal plasma Calcium, Sodium, Zinc and Manganese concentrations were higher (p<0.05, 0.001, 0.01 and 0.05, respectively) in the breeding season than in the non-breeding season, whereas Phosphorus, Chrome, Molybdenum and Boron concentrations were lower (p<0.001, 0.001, 0.05 and 0.001, respectively) in the breeding season than in the non-breeding season. No difference was detected regarding the other elements. The higher levels of Cr and B in the non-breeding season compared to the breeding season both in serum and seminal plasma, regardless of diet intake, suggest that these elements may play a crutial role on male fertility in Merino Ram.Öğe Effects of boron on serum and liver macro elements in rabbits fed with high energy diet(Wiley-Blackwell, 2012) Akalin, P. P.; Baspinar, N.; Basoglu, A.; Sagkan, A.[Abstract Not Available]Öğe Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate in Merino ram(Wiley, 2015) Akalin, P. P.; Akalin, P. Peker; Baspinar, N.; Coyan, K.; Bucak, M. N.; Gungor, U.; Ozturk, C.[Abstract Not Available]Öğe Glucose-6-phosphate dehydrogenase, glutathione peroxidase, total glutatione and reduced nicotinamide adenine dinucleotide phosphate in milk cells of subclinical mastitic cows(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2019) Akalin, P. P.; Ergun, Y.; Baspinar, N.; Dogruer, G.; Kucukgul, A.; Cantekin, Z.; Isgor, M.This study aimed to determine the levels of milk cell total protein (TP), reduced nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), activities of glucose-6-phosphate dehydrogenase (G6PD) and glutathione peroxidase (GPx) in subclinical mastitic cows. Milk from each udder was collected and grouped by the California Mastitis Test. Then, a somatic cell count (SCC) was performed, and the groups were re-scored as control (5-87 x 10(3) cells), 1st group (154-381 x 10(3) cells), 2nd group (418-851 x 10(3) cells), 3rd group (914-1958 x 10(3) cells), and 4th group (2275-8528 x 10(3) cells). Milk cell TP, NADPH, tGSH levels, G6PD, and GPx activities were assessed. Microbiological diagnosis and aerobic mesophyle general organism (AMG, cfu/g) were also conducted. In mastitic milk, TP, NADPH, and tGSH levels, and G6PD and GPx activities were significantly reduced per cell (in samples of 10(6) cells). In addition, milk SCC was positively correlated with AMG (r=0.561, p<0.001), NADPH (r=0.380, p<0.01), TP (r=0.347, p<0.01) and G6PD (r=0.540, p<0.001). There was also positive correlation between NADPH (r=0.428, p<0.01), TP (r=0.638, p<0.001) and AMG. NADPH was positively correlated with TP (r=0.239, p<0.05), GPx (r=0.265, p<0.05) and G6PD (r=0.248, p=0.056). Total protein was positively correlated with tGSH (r=0.354, p<0.01) and G6PD (r=0.643, p<0.001). There was a negative correlation between tGSH and GPx activity (r=-0.306, p<0.05). The microbiological analysis showed the following ratio of pathogens: Coagulase-Negative Staphylococci 66.6%, Streptococcus spp 9.5%, Bacillus spp 9.5%, yeast 4.8%, and mixed infections 9.5%. As a conclusion, when evaluating the enzyme and oxidative stress parameters in milk, it is more suitable to assign values based on cell count rather than ml of milk. The linear correlation between the SCC and AMG, milk cell NADPH, TP and G6PD suggests that these parameters could be used as markers of mastitis.