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  • Küçük Resim
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    The Effects of Different Extenders and Myo-Inositol on Post-thaw Quality of Ram Semen
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2011) Kulaksiz, Recai; Bucak, Mustafa Numan; Akcay, Ergun; Sakin, Fatih; Daskin, Ali; Atessahin, Ahmet
    The study was conducted to evaluate the effects of different extenders and inositol additions on post-thaw semen quality, lipid peroxidation (LPO) and antioxidant activities. Semen was collected from four Karayaka rams from by artificial vagina three times a week. Semen samples showing normospermy quality were pooled. The pooled semen samples were extended in three extenders (Tris, T-, skimmed milk, M-and sodium citrate, NaC) with myo-inositol at two different doses (5 mM, 10 mM) and no antioxidant (control). Nine experimental groups were assigned as follows: T-5I, T-10I, T (control); M-5I, M-10I, M (control); Na-5I, Na-10I, NaC (control). Straws containing extended semen were equilibrated at 4 degrees C for 2 h, frozen in vapor of (15 min at -120 degrees C) liquid nitrogen and stored in liquid nitrogen. Frozen semen was thawed in a water bath at 37 degrees C for 30 seconds. The use of all the extenders supplemented with different doses of myo-inositol did not lead to any significant improvement in microscopic sperm and oxidative stress parameters (P > 0.05). Extenders of T and M resulted in higher sperm motility (50.00+/-2.24% and 55.00+/-0.42%) and HOST (49.00+/-3.32% and 48.17+/-2.97%) rates, compared to NaC (37.00+/-3.74% and 31.80+/-2.96%, P < 0.01), following the freeze/thawing process. Extenders supplementated with myo-inositol not significantly affect malondialdehyde (MDA) levels and activities of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-PX) in comparison to the control groups (P > 0.05), except for MDA level of T extender containing 10 mM inositol. MDA level was found lower (1.22+/-0.07 nmol/ml) in T than those of the M and NaC (P < 0.05). For GSH and GSH-PX activities, T and NaC gave the higher values, compared to M, following the freeze/thawing process (P < 0.01).
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    Öğe
    Fertility comparison of frozen bull semen stored in cryogenic deep freezer (-152 °C) and LN2 container
    (Elsevier Science Inc, 2022) Tirpan, Mehmet Borga; Olgac, Kemal Tuna; Korkmaz, Firat; Sonat, Ali; Kaya, Ufuk; Akcay, Ergun
    The present study aimed to use cryogenic deep freezers that could be a feasible alternative for cryopreserved semen storage. A total of 284 straws from three Simmental bulls and 272 Simmental cows were used. The experimental group consisted of 151 semen straws that were stored at -152 & DEG;C for a week. Moreover, the control group consisted of 133 semen straws that were stored at -196 & DEG;C. Firstly, two samples per bull (n = 6) were examined in terms of sperm kinetic parameters by CASA. Furthermore, plasma membrane, acrosome integrity (PMAI) and high mitochondrial membrane potential (HMMP) were analyzed by flow cytometry. Then, artificial inseminations were performed on Simmental cows with 272 straws belonging to two groups. Then, 56th-day Non-return Rate (NRR56) was determined. All spermatological data were subjected to a linear mixed model. Chi-Square test was performed to NRR56 between storage temperature groups. Also, logistic regression analysis was used to examine the effect of bull, storage temperature and age of cows on pregnancy status. While age of cows was included in the final logistic regression model, effect of bull x storage temperature was not included because it was found as non-significant. The post-thaw PMOT and STR of cryopreserved bull semen, which was stored at -152 & DEG;C, had lower and statistically significant values (p < 0.05). However, frozen bull semen, which were stored at -152 & DEG;C, kept its fertility ability as which stored at -196 & DEG;C. Besides, NRR56 of semen stored at -152 & DEG;C and -196 & DEG;C were detected as 57.24% (83/145) and 55.91% (71/127), respectively (p > 0.05). Nevertheless, these results should be supplemented with more pre-freezing and post-thaw sperm quality analyses and more fertility data for increasing the accuracy of the method. (C)& nbsp;2022 Elsevier Inc. All rights reserved.
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    Öğe
    Relationship between body condition, physiological and biochemical parameters in brown trout (Salmo trutta fario) sperm
    (2006) Bozkurt, Yusuf; Secer, Selcuk; Bukan, Neslihan; Akcay, Ergun; Tekin, Necmettin
    This study investigated the relationships between body condition, seminal plasma composition and physical parameters of Salmo trutta fario in order to find out biomarkers for semen quality. Seminal plasma contained 79.0±15.15 mmol L-1 Na+, 46.8±9.84 mmol L-1 K+, 3.5±0.67 mg dL-1 Ca +4, 3.5±0.80 (mEqL-1 Mg++, 14.8±5.15 mgdl glucose, 3.0±9.42 g dL-1 protein, 19.2±18.57 mg dL-1 cholesterol, 5.4±3.17 mg dL -1 triglyceride and 3.0±9.42 mg dL-1 urea. Semen volume was 3.90±1.48 mL, spermatozoa motility 81.0±10.74%, duration of spermatozoa movement 97.4±15.23 s., spermatozoa density 9432.5±3762.07 ×109 mL-1, total spermatozoa density 35102.4±19137.5 ×109 and semen pH 7.6±0.39. There were significant positive correlations between weight and protein (r=0.752, p<0.05), weight and cholesterol (r = 0.832, p<0.01), length and protein (r = 0.729, p<0.05), length and cholesterol (r = 0.761, p<0.05), volume and cholesterol (r = 0.667, p<0.05), volume and urea (r = 0.753, p<0.05), density and total density (r = 0.704, p<0.05), total density and calcium (r = 0.676, p<0.05), Na+ and K+ (r = 0.822, p<0.01), Mg++ and protein (r = 0.932, p<0.01), protein and cholesterol (r = 0.882, p<0.01), cholesterol and urea (r = 0.885, p<0.01). Significantly negative correlation were found between K+ and pH (r = -0.891, p<0.01), Ca++ and pH (r = -695, p<0.05) and Na+ and urea (r = -0.798, p<0.01). The phenotypic correlation between body weight and length was found highly significant (r = 0.984, p<0.01). © 2006 Asian Network for Scientific Information.