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    The effect of mycorrhiza in nutrient uptake and biomass of cherry rootstocks during acclimatization
    (Ars Docendi, 2010) Aka-Kacar, Yildiz; Akpinar, Cagdas; Agar, Aslihan; Yalcin-Mendi, Yesim; Serce, Sedat; Ortas, Ibrahim
    The effect of arbuscular mycorrhizal fungi (AMF) on growth and nutrient uptake of micropropagated cherry rootstocks was evaluated during acclimatization and plant establishment. Two commonly used cherry rootstocks, 'Edabriz' and 'Gisela 5', were propagated through tissue culture and grown in a greenhouse for 16 weeks. Plantlets were inoculated with Glomus Glomus caledonium, Glomus etunicatum. Glomus intraradices, Glomus mosseae, cocktail (mixture of these species) and indigenous mycorrhiza into three different substrate mixtures. All micropropagated cherry plantlets survived transplanting. After 16 weeks. mycorrhizal plantlets had greater nutrient uptake than non-mycorrhizal plantlets. Roots of inoculated cherry plantlets were heavily colonized with AMF. These results indicated that mycorrhizal inoculation during transplantation from in vitro to ex vitro culture can induce growth responses. The experiments also showed that the mycorrhizal cherry rootstocks were healthier and had higher Zn and P contents when compared to controls for both rootstocks. G. mosseae was one of the most efficient AMF species. Indigenous AMF isolated from Cukurova region also significantly increased the plant growth and nutrient uptake. 'Gisela 5' rootstocks had significantly higher P and Zn contents than 'Edabriz'. Taken together, our results indicate that AMF inoculations enhance growth and development of micropropagated plants which would be beneficial to improve cherry rootstock production.
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    Screening mycorrhiza species for plant growth, P and Zn uptake in pepper seedling grown under greenhouse conditions
    (Elsevier, 2011) Ortas, Ibrahim; Sari, Nebahat; Akpinar, Cagdas; Yetisir, Halit
    We screened the mycorrhizal species for an inoculum protocol would green pepper seedling production and compensate for nutrient deficiency. Three greenhouse studies (on three successive years) were conducted under nursery conditions using five arbuscular mycorrhizal (AM) fungi and their mixture. The AM fungi, Glomus mosseae, G. clarum, G. caledonium, G. intra radices and G. etunicatum propagated on maize roots were used. The AM fungi were inoculated to seed stages and half of the seed stages inoculated plants were re-inoculated at the seedling stages. In Experiment I, plants were harvested once and in Experiments II and III, plants were harvested twice at different developmental stages. Results showed that inoculated plants with G. mosseae, G. intraradices, G. etunicatum, G. clarum, G. caledonium and the mixture of these fungi had increase in shoot, root dry weight. P and Zn content compared to control plants. Mycorrhizal inoculated pepper seedlings flowered earlier than non-inoculated plants. After three successive years, seed and seedling stages inoculation with different AM fungal species varied with the fungal species considered mycorrhizal symbiosis have a different effect on seedling growth was dependent on the fungal species inoculated. Although the mycorrhization increased the seedling quality in all the experiments and the contribution of each individual species varied, this variation was not consistent over the three years. Our results indicate that AM species can be used to compensate for Zn and P deficiency under P and Zn deficient soils for pepper plants. (C) 2010 Elsevier B.V. All rights reserved.