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Öğe Antioxidant and anti-inflammatory activities of Gallic acid in Japanese quails induced by oxidative stress(Univ Zulia, Facultad Ciencias Veterinarias, 2023) Isgor, Mehmet Mustafa; Kucukgul, Altug; Alasahan, SemaGallic acid is a phenolic compound found in many plant sources with strong antioxidant activity. In this study, the bioactivity of Gallic acid was investigated in Japanese quails induced by oxidative stress. The study was performed on four groups of 40-day-old male Japanese quail (Coturnix japonica). Oxidative stress was created for 1 week by adding 0.5% hydrogen peroxide. The study was terminated by administering 100 mg center dot kg(-1) body weight Gallic acid intraperitoneally. Total antioxidant and total oxidant level analyzes from liver tissue homogenates were performed using a ready-made commercial kit. TNF-alpha levels from blood samples taken for anti-inflammatory activity were investigated by ELISA method. There were no statistically significant results on live weight gain between the experimental groups and control group. However, Gallic acid in liver homogenates together with H2O2 increased total antioxidant state (TAS) compared to H2O2 application, while it decreased total oxidant state (TOS) in the same groups. Moreover, while the oxidative stress index increased in the H2O2 group, it decreased significantly in both the Gallic acid and Gallic acid + H2O2 groups. Gallic acid application also caused regression in blood TNF-alpha expression levels, which were increased by H2O2. In quails, Gallic acid showed antioxidant activity by increasing TAS levels and decreasing TOS levels, providing a significant decrease in oxidative stress index. It also provided anti-inflammatory activity by suppressing TNF-alpha levels. However, advanced molecular analyzes are needed to obtain more detailed information on the subject.Öğe Damascus kids' slaughter, carcass and meat quality traits in different production systems using antioxidant supplementation(Elsevier, 2016) Yakan, Akin; Ates, Cafer Tayyar; Alasahan, Sema; Odabasioglu, Fuat; Unal, Necmettin; Ozturk, Oktay Hasan; Gungor, Omer FarukThis study intended to investigate slaughter, carcass and meat quality characteristics of Damascus male kids reared under different production systems and antioxidant effect (Vit E). The kids, housed in pen groups and grazing groups, were equally divided for production systems and later each group was again equally divided for determination of Vit E effect. Production systems and Vit E were found to have no significant effect on slaughter and carcass traits. Differences between production systems were found significant for meat pH(24), water holding capacity, cooking loss, tenderness, ether-extractable lipid and some color characteristics and concentrate feed supplemented with Vit E was effective on TBARS values. Each of the fatty acids except C18:2 n6 was affected by the production system but Vit E influence was superior on long-chain fatty acids. Grazing kids had a lower percentage of total SFA, n6, n6/n3, AI and TI ratio, while kids housed in pens had the lowest percentage of total UFA, NV and n3 ratio. On the other hand, kids that consumed supplemental Vit E had a higher percentage of total UFA, ratio of UFA/SFA, n3 and lower percentage of SFA, ratio of n6/n3, AI, TI compared to the kids fed by non-supplemental concentrate feed with Vit E. In accordance with the meat fatty acid composition, meat obtained from the kids that grazed and consumed supplemental Vit E was healthier than that of those housed in pen kids and non-supplemental Vit E consumed kids. (C) 2016 Elsevier B.V. All rights reserved.Öğe Determination of Egg Quality Characteristics of Different Poultry Species with Digital Image Analysis(Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Alasahan, Sema; Gunlu, AytekinThe present study was aimed at the investigation of egg quality characteristics of different poultry species by digital image analysis. The material of the study was comprised of eggs of the Japanese quail (Coturnix coturnix japonica), rock partridge (Alectoris graeca), ring-necked pheasant (Phasianus colchicus) and cross-chickens (Denizli x Leghorn F1). Values pertaining to egg quality characteristics were obtained using the conventional method and digital image analysis. Egg length, egg width and shape index values measured by digital image analysis were shown to correspond closely with values measured using the conventional method, within an accuracy range of 98.44-98.54%. As regards internal egg quality characteristics, high error rates were determined for albumen height (32.24%) and yolk diameter (13.44%) values measured by digital image analysis, in comparison to values obtained with the conventional method. In result, it was demonstrated that egg quality characteristics could be determined by digital image analysis, in close correspondence with the conventional method, provided that devices equipped with measurement scales and images with reference points are used.Öğe Effects of black cumin (Nigella sativa L.) seed on growth performance, blood parameters, liver oxidant/anti-oxidant levels and fatty liver syndrome in quails(Urmia Univ, 2023) Cimrin, Tulay; Alasahan, Sema; Kazak, Filiz; Kutlu, Tuncer; Kisacam, Mehmet AliThis research aimed to evaluate the effect of different doses of black cumin (Nigella sativa L.) seed (BCS) on growth performance, blood parameters, liver oxidant/anti-oxidant levels and fatty liver syndrome in quails. Four hundred and thirty-two unsexed (male and female) three-day-old Japanese quail (Coturnix coturnix japonica) chicks were divided into four treatment groups (108 chicks per group) with six replicates (18 chicks per replicate). Control and experimental groups were fed for 35 days with basal quail feed including 0.00, 0.50, 1.00 and 2.00% BCS supplement, respectively. At the end, a total of 96 quails, 24 from each group (12 females and 12 males) were slaughtered. The BCS-addition did not affect the growth performance in the experimental groups compared to the control group. Addition of BCS to the diet significantly decreased serum aspartate aminotransferase, lactate dehydrogenase and urea amounts compared to the controls. Whereas, cholesterol decreased significantly with the addition of only 1.00% and low-density lipoprotein with the addition of 0.50 and 1.00% BCS compared to the controls. Liver glutathione levels significantly elevated in 0.50 and 1.00% BCS fed groups; while, glutathione peroxidase levels significantly decreased in 1.00 and 2.00% BCS fed groups. Adding 1.00 and 2.00% BCS to the feed reduced fatty liver incidence in male quails. It is concluded that adding 0.50 and 1.00% BCS positively affects the blood and liver parameters; therefore, BCS may be suggested as an anti-oxidant source to help protect hepatocytes against tissue damage as it has a significant effect on maintaining oxidant and anti-oxidant balance.(c) 2023 Urmia University. All rights reserved.Öğe The impact of eggshell colour and spot area in Japanese quails: I. eggshell temperature during incubation and hatching results(Revista Brasileira Zootecnia Brazilian Journal Animal Sci, 2016) Alasahan, Sema; Akpinar, Gulsen Copur; Canogullari, Sibel; Baylan, MikailThis study was carried out to investigate the effects of eggshell colour and spot properties (colour and size of the spot area) on eggshell incubation temperature and hatching outcomes of Japanese quail eggs (Coturnix coturnix japonica). Study material was allocated to five groups according to their eggshell and spot colours: black spots on greyish white coloured eggshell (I), blue spots on greyish white coloured eggshell (II), diffuse brown spots on greyish brown coloured eggshell (III), brown spots on light green coloured eggshell (IV), and small brown spots on greyish brown coloured eggshell (V). The size of the spotted area was determined in each egg group using digital image analysis. Mean relative weight losses of hatched and unhatched eggs between days 0-10 and 0-14 of embryonic development were 4.76% and 10.48% and 9.17% and 15.46%, respectively. The mean eggshell temperatures of hatched and unhatched eggs measured at the equatorial region on days 10 and 14 during embryonic development were 36.92 and 37.79 degrees C and 36.84 and 37.18 degrees C, respectively. Eggshell temperatures at the equatorial region on days 10 (36.89 degrees C) and 14 (37.57 degrees C) of embryonic development were lower than the fixed temperature of the incubator (37.6 degrees C). Fertility, hatchability of fertile eggs, and hatchability and embryonic mortality rates do not vary in relation to eggshell colour or the size of the spotted area.Öğe The impact of eggshell colour and spot area in Japanese quails: II. Slaughter and carcass characteristic(Revista Brasileira Zootecnia Brazilian Journal Animal Sci, 2016) Alasahan, Sema; Akpinar, Gulsen Copur; Canogullari, Sibel; Baylan, MikailThis study was carried out to investigate the effects of eggshell colour and spot properties (colour and size of the spot area) on growth performance and carcass traits of Japanese quail (Coturnix coturnix japonica) eggs. Study material were allocated to five groups according to their eggshell and spot colours: black spots on greyish white coloured eggshell (I), blue spots on greyish white coloured eggshell (II), diffuse brown spots on greyish brown coloured eggshell (III), brown spots on light green colored eggshell (IV), and small brown spots on greyish brown coloured eggshell (V). The size of the spotted area was determined in each egg group using digital image analysis. The groups did not differ for body weight and length of the shank at the end of the growth period. However, the groups differed significantly for carcass yield after slaughter (not eviscerated) and carcass yield. These parameters were highest in Group I (82.08 and 76.09%) and lowest in Group III (80.20 and 73.86%). Digital image analysis demonstrated that heart length, cardiac fat area, gizzard width, and intestine length varied between the groups. Cardiac fat area was largest in Group III (0.86 cm(2)) and smallest in Group V (0.65 cm(2)). Gizzard width was greatest in Group I (2.63 cm) and smallest in Group V (2.47 cm). Intestine length was greatest in Group V (78.45 cm) and smallest in Group IV (72.39 cm). Body weight, shank length, and slaughter and carcass weight do not vary in relation to eggshell colour or the size of the spotted area. The lengths of intestine and heart, gizzard width, and cardiac fat area do vary in relation to eggshell colour or the size of the spotted area.Öğe Influence of eggshell colour on egg yolk antibody level, incubation results, and growth in broiler breeders(Revista Brasileira Zootecnia Brazilian Journal Animal Sci, 2017) Baylan, Mikail; Celik, Ladine Baykal; Akpinar, Gulsen Copur; Alasahan, Sema; Kucukgul, Altug; Dogan, Sibel CanogullariThis study was performed to determine the effect of shell colour in eggs acquired from Ross-308 broiler breeders on the interior and exterior quality of the egg, the antibody content of the egg yolk, and growth performance. The shell colours of a total of 1350 eggs were classified, using a colorimeter, into three groups: dark (E < 64), medium (E: 64.00-67.00), and light (E > 67). The difference between groups with respect to egg weight, shape index, shell weight, and Haugh unit value was significant. Egg yolk antibody content (IgY) was 6.658 mg/mL in the dark colour group, 5.130 mg/mL in the medium colour group, and 5.242 mg/mL in the light colour group. Among incubation characteristics, the fertility rate as, in order, 94.66%, 92.14%, and 87.92% in dark, medium, and light shell colour eggs, and the hatchability was 87.00%, 84.28%, and 80.57%, in the same order. No significant difference was observed between groups with respect to hatchability of fertile eggs and embryonic mortality rates. No significant difference was observed between groups for live weight, feed intake, and feed conversion ratio either. The eggshell colour has an effect on yolk antibody content and on the hatchability, but it has no influence on hatchability of fertile eggs, Haugh unit and growth performance.