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Öğe Erythrocyte and spermatozoa glucose-6-nhosphate dehydrogenase activity in merino rams: An experimental study(Shahid Sadoughi Univ Medical Sciences, 2018) Gurel, Huseyin; Baspinar, Nuri; Akalin, Pinar Peker; Altunok, Vahdettin; Kazak, FilizBackground: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate metabolic pathway that supplies reducing agents by maintaining the level of reduced nicotinamide adenine dinucleotide phosphate. Objective: It was aimed to determine the activity of erythrocyte and spermatozoa G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find out the relation of these parameters with sperm quality parameters for better understanding the role of this enzyme in male fertility. Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved. Ejaculate samples were collected using an artificial vagina, in October (the breeding season) and April (the non-breeding season). Blood samples were collected prior to sperm collection. Sperm volume (ml), motility (%), mass activity (1-5), concentration (x10(6)), viability (%), abnormal acrosome morphology (%) and abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and erythrocyte G6PD were determined and the relation of sperm parameters with G6PD activity was evaluated. Results: Erythrocyte G6PD activity was higher (p <= 0.001), whereas spermatozoa G6PD activity was lower (p<0.001) in the breeding season (1.928 +/- 0.231 U/g hemoglobin, 129.65 +/- 28.41 U/g protein, respectively) from that in the non-breeding (0.530 +/- 0.066 U/g hemoglobin, 562.36 +/- 94.92 U/g protein, respectively). There were also significant differences among sperm quality parameters within the seasons. Positive correlation was determined between spermatozoa G6PD activity (r=0.053, p=0.03 and sperm concentration in the breeding season. Conclusion: Higher spermatozoa G6PD activity in October, where the level of polyunsaturated fatty acids is suggested to be increased, may reflect the increased need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity for the oxidative balance.Öğe Erythrocyte and spermatozoa glucose-6-phosphate dehydrogenase activity in merino rams: An experimental study(Research and Clinical Center for Infertitlity, 2018) Gurel, Hüseyin; Baspinar, Nuri; Akalin, Pınar Peker; Altunok, Vahdettin; Kazak, FilizBackground: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate metabolic pathway that supplies reducing agents by maintaining the level of reduced nicotinamide adenine dinucleotide phosphate. Objective: It was aimed to determine the activity of erythrocyte and spermatozoa G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find out the relation of these parameters with sperm quality parameters for better understanding the role of this enzyme in male fertility. Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved. Ejaculate samples were collected using an artificial vagina, in October (the breeding season) and April (the non-breeding season). Blood samples were collected prior to sperm collection. Sperm volume (ml), motility (%), mass activity (1-5), concentration (×106), viability (%), abnormal acrosome morphology (%) and abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and erythrocyte G6PD were determined and the relation of sperm parameters with G6PD activity was evaluated. Results: Erythrocyte G6PD activity was higher (p?0.001), whereas spermatozoa G6PD activity was lower (p?0.001) in the breeding season (1.928±0.231 U/g hemoglobin, 129.65±28.41 U/g protein, respectively) from that in the non-breeding (0.530±0.066 U/g hemoglobin, 562.36±94.92 U/g protein, respectively). There were also significant differences among sperm quality parameters within the seasons. Positive correlation was determined between spermatozoa G6PD activity (r=0.053, p=0.03 and sperm concentration in the breeding season. Conclusion: Higher spermatozoa G6PD activity in October, where the level of polyunsaturated fatty acids is suggested to be increased, may reflect the increased need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity for the oxidative balance. © 2018, Research and Clinical Center for Infertitlity. All rights reserved.Öğe Immunoglobulins and acute phase proteins in Van cats - associations with sex, age, and eye colour(Tubitak Scientific & Technological Research Council Turkey, 2021) Coskun, Pinar; Altunok, Vahdettin; Kazak, Filiz; Yuksek, NazmiIt was aimed to determine concentrations of plasma immunoglobulins (IgG, IgA, IgM) and acute phase proteins (alpha-1 acid glycoprotein, serum amyloid A, ceruloplasmin) and the association of these parameters with age, sex, and eye colour in Van cats. Blood plasma of healthy, forty-seven Van cats (Van Cat Home) fed with standard cat food were involved in the study. Cats were divided into four groups based on age (<1, 2 to 2.5, 3 to 4, > 5) and eye colour (amber-amber, amber-blue, blue-amber, and blue-blue eyes described from left to right), and two groups based on sex (male and female). Plasma IgG, IgA, IgM, alpha-1 acid glycoprotein, serum amyloid A, and ceruloplasmin concentrations were determined, and the concentrations were found to be 2.20 +/- 0.05 mg/mL, 1.05 +/- 0.04 mg/mL, 2.52 +/- 0.18 mg/mL, 562.00 +/- 14.27 mu g/mL, 1.49 +/- 0.03 mu g/mL, and 2.88 +/- 0.13 mg/dL, respectively. Male Van cats had higher IgA levels than the female cats (P < 0.05). alpha-1 acid glycoprotein concentrations in cats under 1-year-old were higher from that in the other age groups, whereas the significant difference was obtained compared to 2 to 2.5-year-old cats (p < 0.05). IgM concentrations were higher in cats with blue-blue eye colour than in the cats with other eye colours, while statistically significant difference was observed only cats with amber-blue eye colour (p < 0.05). Amber-amber eyed cat plasma ceruloplasmin concentrations were higher from that in the other eye coloured cats while significant difference was determined compared to amber-blue eyed cats (p < 0.05). Plasma immunoglobulins and acute phase proteins were determined and the associations of these parameters with age, sex, and eye colour were examined for the first time in Van cats. These data are thought to be important as reference levels in terms of diagnosis and prognosis.Öğe Relation of some biochemical parameters in Van (Turkey) cats with sex, age, eye colour and hair length(2018) Altunok, Vahdettin; Başpınar, Nuri; Peker Akalın, Pınar; Yüksek, NazmiAmaç: Van kedileri, doğu Türkiye kaynaklıdır ve ulusal kültürel varlıkların bir parçasıdır. Van kedilerinin en belirgin özelliği, gözlerinin renkleridir (diskromatopsi). Bu çalışmanın amacı, Van kedilerinde plazma aspartat aminotransferaz, alanin aminotransferaz aktiviteleri, total protein, albümin, glikoz, trigliserit, LDL kolesterol, HDL kolestrol, kan üre / nitrojen ve kreatinin düzeylerini saptamak ve bu parametrelerin cinsiyet, yaş, göz rengi ve tüy uzunluğu ile ilişkisini araştırmaktır. Gereç ve Yöntem: Bu çalışmada, standart kedi yemi ile beslenen, sağlıklı, 47 adet Van kedisi (Van Kedi Evi, Yuzuncu Yil Üniversitesi, Van) kan plazması kullanıldı. Kediler, cinsiyet (dişierkek), yaş (1-2; 3-4; 5'in üzerinde), göz rengi (amber-amber, amber-mavi, mavi-amber, mavi-mavi, soldan sağa açıklanan gözler) ve tüy uzunluğu (kısa; uzun) temel alınarak gruplara ayrıldı. Plazma biyokimyasal parametreleri ticari kitlerle kolorimetrik olarak spektrofotometre ile belirlendi.Bulgular: Cinsiyete ilişkin olarak parametreler arasında fark yoktu. Kısa tüylü kedilerde uzun tüylü kedilere göre albumin düzeyleri daha yüksekti (p<0.05). Göz rengi açısından değerlendirildiğinde, mavi-mavi gözlü kedi plazma glikoz düzeyleri diğer göz rengi gruplarına göre çok daha yüksekti (p<0.001). Yaş gruplarında, 1-2 yaşındaki kedilerde kreatinin düzeyleri diğer yaş gruplarına göre daha düşüktü (p<0.05)Öneri: Sonuçlarımız, Van kedilerinin plazma biyokimyasal parametrelerinin bazılarının göz rengi, yaş ve tüy uzunluğu ile ilişkili olabileceğini göstermiştir. Ayrıca Van kedilerinden elde edilen bu biyokimyasal bulguların, klinik tanıda yarar sağlayacağı düşünülmektedir.
