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    AFLP analysis of genetic variation within the two economically important Anatolian grapevine (Vitis vinifera L.) varietal groups
    (Canadian Science Publishing, Nrc Research Press, 2006) Ergul, Ali; Kazan, Kemal; Aras, Sumer; Cevik, Volkan; Celik, Hasan; Soylemezoglu, Gokhan
    The Anatolian region of modern-day Turkey is believed to have played an important role in the history of grapevine (Vitis vinifera L.) domestication and spread. Despite this, the rich grape germplasm of this region is virtually uncharacterized genetically. In this study, the amplified fragment length polymorphisms (AFLP)-based genetic relations of the grapevine accessions belonging to the 2 economically important Anatolian table grape varietal groups known as V. vinifera 'Misket' (Muscat) and V. vinifera 'Parmak' were studied. Thirteen AFLP primer combinations used in the analyses revealed a total of 1495 (35.5% polymorphic) and 1567 (34.6% polymorphic) DNA fragments for the 'Misket' and 'Parmak' varietal groups, respectively. The unweighted pair-group method with arthimetic averaging (UPGMA) cluster analysis and principal coordinate analysis (PCA) conducted on polymorphic AFLP markers showed that both varietal groups contain a number of synonymous (similar genotypes known by different names) as well as homony mous (genetically different genotypes known by the same name) accessions. Our results also showed that 6 of the Anatolian 'Misket' genotypes were genetically very similar to V. vinifera 'Muscat of Alexandria', implying that these genotypes might have played some role in the formation of this universally known grape cultivar. Finally, the close genetic similarities found here between 'Muscat of Alexandria' and V. vinifera 'Muscat of Hamburg' support the recent suggestion that 'Muscat of Hamburg' probably originated from 'Muscat of Alexandria' through spontaneous hybridizations. Overall, the results of this study have implications for not only preservation and use of the Anatolian grape germplasm, but also better understanding of the historical role that this region has played during the domestication of grapes.
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    Genome-wide identification of salinity responsive HSP70s in common bean
    (Springer, 2016) Buyuk, Ilker; Inal, Behcet; Ilhan, Emre; Tanriseven, Mehmet; Aras, Sumer; Erayman, Mustafa
    The present study is aimed to identify and characterize HSP70 (PvHSP70) genes in two different common bean cultivars under salt stress. For this purpose various in silico methods such as RNAseq data and qRT-PCR analysis were used. A total of 24 candidate PvHSP70 gene were identified. Except for chromosome 4 and 7, these candidate PvHSP70 genes were distributed on the remaining chromosomes. While the lowest number of PvHSP70 genes was determined on chromosomes 1, 3, 5, 7, 9, 10 and 11 (one HSP70 gene), the highest number of PvHSP70s was on chromosomes 6 and 8 (seven HSP70 genes each). Three genes; PvHSP70-5, -9, and -10 were found to have no-introns. In addition, four tandemly and six segmentally duplicated gene couples were detected. A total of 13 PvHSP70 genes were targeted by miRNAs of 44 plant species and the most targeted genes were PvHSP70-5 and -23. The expression profile of PvHSP70 genes based on publicly available RNA-seq data was identified and salt treated leaf tissue was found to have more gene expression levels compared to the root. qRT-PCR analysis showed that the transcript concentrations of upregulated PvHSP70 genes in leaves of Zulbiye (sensitive) were mostly higher than those of Yakutiye (resistant). The present study revealed that PvHSP70 genes might play an important role in salt stress response for common bean cultivars and variability between cultivars also suggests that these genes could be used as functional markers for salt tolerance in common bean.
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    Molecular diversity in persimmon (Diospyros kaki L.) cultivars growing around Hatay province in Turkey
    (Academic Journals, 2007) Yÿldÿz, Merve; Bayazit, Safder; Cebesoy, Suna; Aras, Sumer
    Genetic relationships among Diospyros kaki L. growing around Hatay province in Turkey were assessed by randomly amplified polymorphic DNA (RAPD) assay. Ten decamer primers were selected from 50 primers. These primers yielded a total of 155 bands and 126 of them were recorded as polymorphic. Pairwise genetic distances of the samples were used to construct dendograms using Unweighted Pair-Group Method of Arithmetic Average (UPGMA). The study suggests that the morphological differences among cultivars of persimmon might be the result of genetic differences rather than the ecological or growing conditions. The study forms a basic contribution to the characterization of D. kaki population in Turkey.