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Öğe Iron-Induced Cerebellar Purkinje Cell Loss Is Ameliorated by Flunarizine(Tubitak Scientific & Technological Research Council Turkey, 2009) Kozan, Ramazan; Bostanci, M. Oemer; Nacar, Tuncer; Aslan, Ali; Bagirici, FarukAim: In this study, we investigated the effect of intracerebroventricular-injected iron neurotoxicity on the total number of cerebellar Purkiinje cells in rats and the possible neuroprotective effect of flunarizine, a piperazine-derived calcium channel blocker. Materials and Methods: Rats were divided into four groups: control, flunarizine. iron, and iron + flunarizine groups. Rats in the iron and iron + flunarizine groups received intracerebro-ventricular iron (FeCl36H2O. 200 mM, 2.5 mu l), while those in flunarizine and iron + flunarizine groups were intraperitoneally injected with flunarizine (10 mg/kg/day) once a day after the operation for 10 days. After 10 days, all rats were perfused intracardially and then sacrificed. Brain tissues were removed and standard histological techniques were performed. The total numbers of Purkinje cells were estimated using unbiased stereological techniques. Results: Means of the total numbers of Purkinje cells in the cerebellum were estimated as 310441 +/- 6558, 298658 +/- 9636, 200201 +/- 6822 and 282658 +/- 6327 in the control, flunarizine, iron, and iron + flunarizine groups. respectively. Comparison between iron and iron + flunarizine groups revealed that flunarizine significantly attenuates the iron-induced neuron loss from 35.5% to 8.9% (P < 0.05). Conclusions: Findings of the present study suggest that flunarizine has a neuroprotective effect on iron-induced Purkinje cell loss in the rat cerebellum via blocking influx of calcium ions into neurons.Öğe Iron-induced cerebellar purkinje cell loss is ameliorated by flunarizine(2009) Kozan, Ramazan; Bostancı, Mehmet Ömer; Nacar, Tuncer; Aslan, Ali; Bağırıcı, FarukAim: In this study, we investigated the effect of intracerebroventricular-injected iron neurotoxicity on the total number of cerebellar Purkinje cells in rats and the possible neuroprotective effect of flunarizine, a piperazinederived calcium channel blocker. Materials and Methods: Rats were divided into four groups: control, flunarizine, iron, and iron + flunarizine groups. Rats in the iron and iron + flunarizine groups received intracerebro-ventricular iron (FeCl36H2O, 200 mM, 2.5 μl), while those in flunarizine and iron + flunarizine groups were intraperitoneally injected with flunarizine (10 mg/kg/day) once a day after the operation for 10 days. After 10 days, all rats were perfused intracardially and then sacrificed. Brain tissues were removed and standard histological techniques were performed. The total numbers of Purkinje cells were estimated using unbiased stereological techniques. Results: Means of the total numbers of Purkinje cells in the cerebellum were estimated as 310441 ± 6558, 298658 ± 9636, 200201 ± 6822 and 282658 ± 6327 in the control, flunarizine, iron, and iron + flunarizine groups, respectively. Comparison between iron and iron + flunarizine groups revealed that flunarizine significantly attenuates the iron-induced neuron loss from 35.5% to 8.9% (P < 0.05). Conclusions: Findings of the present study suggest that flunarizine has a neuroprotective effect on ironinduced Purkinje cell loss in the rat cerebellum via blocking influx of calcium ions into neurons.Öğe PHENOLIC CONTENT AND BIOLOGICAL ACTIVITIES OF TRAMETES HIRSUTA(Parlar Scientific Publications (P S P), 2021) Akgul, Hasan; Aslan, Ali; Akata, Ilgaz; Gunal, Selami; Bal, Celal; Baba, HayriNatural mushrooms are one of the important elements of the ecosystem. It has been preferred by people from past to present because of its nutritional and medicinal potentials. In this study, antioxidant, oxidant and antimicrobial potential of Trametes hirsuta (Wulfen) Lloyd has been determined. In addition, phenolic compounds in its fruiting body have been scanned. The antioxidant and oxidant potential was determined using Rel Assay kits. Antimicrobial potential was determined using the agar dilution method. Phenolic contents were scanned on the HPLC device. As a result of the studies, the presence of different levels of Gallic acid, Catechin, 4-Hydroxybenzoic acid and Epicatechin were observed in T. hirsuta. TAS value of T hirsuta was determined 3.466 +/- 0.148 mmol/L, TOS value was 13.482 +/- 0.234 mu mol/L and OSI value was 0.390 +/- 0.018. Antimicrobial activity of T. hirsuta was found to be effective at 100, 200 and 400 mu g/mL concentrations. As a result of the studies, it has been determined that T hirsuta has antioxidant and antimicrobial potential.Öğe Sıçan serebellumunda demir nörotoksisitesine karşı vitamin E'nin koruyucu etkisi(2009) Kozan, Ramazan; Bostancı, Mehmet Ömer; Ayas, Bülent; Aslan, Ali; Bağırıcı, FarukAmaç: Bu çalışmada, intraserebroventriküler olarak verilen demirin, sıçan serebellar Purkinje hücrelerinde oluşturduğu nörotoksisiteye karşı yağda çözülebilen güçlü bir antioksidan olan vitamin E'nin (?-tokoferol) etkisini araştırdık. Gereç ve Yöntem: Sıçanlar, kontrol, demir ve demir+vitamin E grupları olmak üzere üç gruba ayrıldı. Demir ve demir+vitamin E gruplarındaki sıçanlara intraserebroventriküler olarak demir (FeCl36H2O, 200 mM, 2.5 ?l) verildi. Demir+vitamin E grubundaki sıçanlara operasyonu takiben on gün süreyle 100 mg/kg/gün dozunda vitamin E intraperitoneal olarak verildi. Onuncu günün sonunda, bütün gruplardaki hayvanlar intrakardiyak yolla perfüze edildikten sonra dekapite edildiler. Beyin dokuları çıkarılarak standart histolojik doku takibi uygulandı. Toplam Purkinje hücre sayıları tarafsız sayım metodu olan stereolojik yöntemle hesaplandı. Bulgular: Ortalama toplam Purkinje hücre sayıları, kontrol grubunda 490584±13286; demir grubunda 331497±10764 ve demir+vitamin E grubunda 412118±15842 olarak bulundu. Demir ve demir+vitamin E grupları karşılaştırıldığında, vitamin E'nin demirin indüklediği Purkinje hücre kaybını %32.4'den %15.9'a düşürdüğü bulundu (p<0.01). Sonuç: Bu çalışmanın sonuçları, vitamin E'nin sıçan serebellumunda demirin indüklediği Purkinje hücre kaybını dikkate değer ölçüde geri çevirdiğini gösterdi. Bu nöroprotektif etkinin, vitamin E'nin serbest radikaller üzerine antioksidan aktivitesinden kaynaklandığı düşünülmektedir.
