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  • Küçük Resim
    Öğe
    Bruselloz hastalarında serum nitrik oksit ve malondialdehit düzeyleri
    (2005) Savaş, Lütfü; Önlen, Yusuf; Savaş, Nazan; Polat, Gürbüz; Aslan, Gönül
    Amaç: Bu çalışmanın amacı bruselloz hastalarında serum nitrik oksit (NO) ve malondialdehit (MDA) düzeylerini saptamak; bulunan NO ile MDA değerlerini, eritrosit sedimentasyon hızı (ESR), aspartat aminotransferaz (AST), alanin aminotransferaz (ALT) gibi laboratuvar testleri ve ateş, terleme, eklem ağrısı gibi klinik bulgularla karşılaştırmaktı. Gereç ve Yöntemler: Bu çalışmada 39 brusellozlu hastada tedaviden önce ve kontrol grubu olarak 20 sağlıklı kişide NO ve MDA düzeyleri araştırıldı. NO düzeylerini saptamak için Green yöntemi ile nitrat düzeyleri ölçüldü. MDA düzeylerini saptamak için Yagi metodu kullanıldı. Bulgular: Brusellozlu hastalarda serum NO ve MDA düzeyleri kontrol grubuna göre istatistiksel olarak anlamlı derecede yüksek bulundu (p< 0.05). NO ile brusella serum aglütinasyon testi (SAT), AST, ALT, ateş, terleme ve eklem ağrısı arasında anlamlı bir ilişki yoktu. Buna karşılık NO düzeyleri yüksek olanlarda ESR değerleri yüksekti (p< 0.05). Yine MDA ile ESR, ALT, SAT, ateş ve eklem ağrısı arasında anlamlı bir ilişki bulunmazken (p> 0.05), AST 40 IU/L’nin üzerinde olanlarda MDA değerleri düşük bulundu (p< 0.05). Ayrıca terleme öyküsü olanlarda MDA değerleri yüksekti (p< 0.05). Sonuç: Bu yüksek NO ve MDA değerlerinin klinik olarak anlamlı olup olmadığını göstermek için bu konuda daha fazla klinik çalışmaya ihtiyaç vardır.
  • Küçük Resim
    Öğe
    Numerical analysis of Candida species from uriner system infections based on SDS-PAGE and detection of antifungal resistance
    (TIP ARASTIRMALARI DERNEGI, 2007) Duran, Nizami; Öztürk, Fatma; Açik, Leyla; Aslantaş, Özkan; Aslan, Gönül
    Aim: The aim of the present study was to evaluate the protein patterns and numerical analysis of Candida species isolates from urinARY system infections by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), and to detect antifungal susceptibility. Methods: In this study, 96 Candida spp. belonging to 46 strains of C.albicans, 18 strains of C. glabrata, 14 strains of C. tropicalis, 8 strains of C. krusei, 6 strains of C. parapsilosis, 3 strains of C. dubliensis and 1 strain of C. kefyr from urine samples or urinary system infections were examined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The susceptibility of Candida strains against antifungal agents were assessed by minimal inhibitory concentrations (MIC). Results: Nineteen distinct band patterns in the range of 63-120 kDa were obtained. Different isolates of each species were clearly different in each of the seven species. But, some variation in the protein patterns was detected within the same species. Conclusion: The whole-cell protein profiles performed by SDS-PAGE associated with computer-assisted numerical analysis may provide preliminary criteria for taxonomic and epidemiological studies of such microorganisms.
  • [ N/A ]
    Öğe
    Serum levels of nitric oxide and malondialdehyde in patients with brucellosis
    (Turkiye Klinikleri, 2005) Savaş, Lütfü; Önlen, Yusuf; Savaş, Nazan; Polat, Gürbüz; Aslan, Gönül
    Objective: The aim of this study was to determine serum levels of nitric oxide (NO) and malondialdehyde (MDA) in patients with brucellosis and to compare these levels with laboratory tests such as erythrocyte sedimentation rate (ESR), aspartate aminotransferase (AST), alanine aminotransterase (ALT), brucella serum agglutination test (SAT), as well as clinical findings such as fever, sweating, and arthricular pain for the diagnosis of brucellosis. Material and Methods: In this study, serum NO and MDA levels were determined in 39 patients with brucellosis before treatment, and in 20 healthy individuals as a control group. Nitrate levels were measured by Green's method, and Yagi's method was used to determine MDA levels. Results: Serum NO and MDA levels were significantly raised in patients with brucellosis when compared to those of the control group (p< 0.05). There was no relation between serum levels of NO, SAT, AST, ALT, or fever, sweat, and arthricular pain. Contrarily, the ESR was also high in patients with high levels of NO (p< 0.05). In addition, there was no relation between MDA and ESR, ALT, SAT, fever, and arthricular pain (p> 0.05); however, in patients with AST levels higher than 40 IU/L, levels of MDA were low (p< 0.05). Furthermore, MDA levels were high in patients with a history of sweating (p< 0.05). Conclusion: Further studies are needed in order to determine whether high levels of NO and MDA in patients with brucellosis are clinically significant. Copyright © 2005 by Türkiye Klinikleri.