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    Antimicrobial, Antioxidant Activity of the Essential Oil of Bay Laurel from Hatay, Turkey
    (Taylor & Francis Ltd, 2013) Yilmaz, Ebru Sebnem; Timur, Mahir; Aslim, Belma
    The aim of this study was to evaluate the antimicrobial and antioxidant activity of the essential oil (EO) extracted from the leaves of Laurus nobilis L. The oil was analyzed by gas chromatography and mass spectrometry (GC-MS). Twenty seven components were identified, representing 96.6 % of the EO. The main compounds identified were 1,8-cineole (51.8 %), alpha-terpinyl acetate (11.2 %), and sabinene (10.1 %). The oil was screened for possible antioxidant activity using two complementary test systems: DPPH (2,2-diphenylpicrylhydrazyl) free radical-scavenging and the beta-carotene/linoleic acid assay. Both of these in vitro methods showed that the EO was a less powerful reducing agent than the well-known synthetic antioxidants, butylated hydroxytoluene and ascorbic acid. Also, the antimicrobial activity of the EO was tested against a panel of food-spoiling bacteria and one yeast strain. The minimum inhibitory concentration values for microorganisms that were sensitive to L. nobilis EO ranged from 125-2000 mu g/mL.
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    POTENTIAL OF NICKEL(II) REMOVAL BY SYNECHOCYSTIS SP ISOLATES
    (Parlar Scientific Publications (P S P), 2012) Yilmaz, Ebru Sebnem; Aslim, Belma; Cansunar, Emir
    The removal of heavy metal ions by microorganisms may present an alternative method for their removal from wastewater. In this study, 10 cyanobacteria were isolated from Kucukcekmece Lagoon (Turkey). All the isolates were examined for nickel(II) concentrations in the range of 5-25 mg/L. EC50 values of the isolates were determined by probit analysis. Synechocystis sp. BASO403 (EC50 of 17.41 mg/L) and Synechocystis sp. BASO404 (EC50 of 2.56 mg/L) were selected among the isolates based on their capacity to produce extracellular polysaccharides (EPS; 143 mg/L and 44 mg/L), respectively, for removal at 15 mg/L Ni(II) concentration. The BASO404 isolate displayed a higher Ni(II) removal (95%), especially on the cell surface, than BASO403 (51%) after a 7-days incubation period. A reverse relationship between EPS capacity and Ni removal (p<0.01) was indicated, but a positive correlation was observed between EPS capacity and Ni toxicity (p<0.01). It is suggested that EPS from two isolates of Synechocystis sp. play a protective role for Ni(II) toxicity.
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    Potential of nickel(II) removal by synechocystis sp. Isolates
    (2012) Yilmaz, Ebru Sebnem; Aslim, Belma; Cansunar, Emir
    The removal of heavy metal ions by microorganisms may present an alternative method for their removal from wastewater. In this study, 10 cyanobacteria were isolated from Kucukcekmece Lagoon (Turkey). All the isolates were examined for nickel(II) concentrations in the range of 5-25 mg/L. EC 50 values of the isolates were determined by probit analysis. Synechocystis sp. BASO403 (EC50 of 17.41 mg/L) and Synechocystis sp. BASO404 (EC50 of 2.56 mg/L) were selected among the isolates based on their capacity to produce extracellular polysaccharides (EPS; 143 mg/L and 44 mg/L), respectively, for removal at 15 mg/L Ni(II) concentration. The BASO404 isolate displayed a higher Ni(II) removal (95%), especially on the cell surface, than BASO403 (51%) after a 7-days incubation period. A reverse relationship between EPS capacity and Ni removal (p<0.01) was indicated, but a positive correlation was observed between EPS capacity and Ni toxicity (p<0.01). It is suggested that EPS from two isolates of Synechocystis sp. play a protective role for Ni(II) toxicity. © by PSP.