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The determination of Alpha Naphthyl Acetate Esterase (ANAE) activities in the peripheral blood leukocytes of geese (Anser anser)
(2009) Sari, E. Karadag; Kozlu, T.; Kocamis, H.; Deprem, T.; Asti, R.N.; Nazli, M.
The objectives of this study were to investigate the presence of the Alpha Naphthyl Acetate Esterase (ANAE) in peripheral blood leukocytes in goose and to determine the proportions of blood T lymphocytes with this method. Blood smears obtained from 15 geese, 10 month old, were incubated at various pHs (5.0, 5.4, 5.8, 6.2, 6.4, 6.8 and 7.2) for 3 or 6 hours with the enzyme substrate. The highest resolution and consequently the best determination of positive ANAE cells were obtained with pH 5.8 for an incubation period of 6 hours. Positive ANAE activity was only recorded in mono-cytes and in some lymphocytes (T cells) according a diffuse and a granular pattern respectively whereas granulocytes, thrombocytes and B lymphocytes appeared ANAE negative. The mean ratio of ANAE positive lymphocytes in peripheral blood in goose was 53.6 ± 3.2% and exhibited weak inter-individual fluctuations (coefficient of variations: 5.9%). These results suggest that ANAE staining method could be easily and efficiently used for T cell counting in peripheral blood.