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    The genetic structure of the goat breeds belonging to Northwest part of Fertile Crescent
    (Elsevier, 2020) Gul, Sabri; Yilmaz, Onur; Gunduz, Zuhal; Keskin, Mahmut; Cemal, Ibrahim; Ata, Nezih; Onel, Suleyman Ercument
    The present study was performed to reveal genetic diversity and population structure of four native Turkish goat breeds (Hatay, Kilis, Shami and Hair), raised in Hatay and Kilis provinces located in northwest part of Fertile Crescent, using twenty-two microsatellite markers. Animal material for the study was consisted of 246 goats from Hair (60), Shami (62), Kilis (64) and Hatay (60) breeds. A total of 458 alleles were detected from twenty two markers studied. Results obtained for the mean number of alleles (20.82), expected heterozygosity (0.89) and polymorphic information content (0.88) indicated that the total analyzed population is characterized by noticeable genetic variability. The value obtained for the global coefficient of gene differentiation showed that the majority of the total genetic variation is due to individual differences (97.50%). Clustering analysis divided native goat breeds raised in Fertile Crescent into two clusters. The first cluster was consisted by Kilis and Hatay goat breeds, the second cluster was formed by Shami and Hair goat breeds. Results indicated that Kilis goat and Hatay goats are genetically closer than the other studied breeds. The results obtained from the present study will be helpful for constructing suitable selection program for indigenous goat breeds studied. On the other hand, obtained information on genetic relationships between other goat breeds raised in the region and Hatay goat is very valuable to be registered as a national breed.
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    Microsatellite panels for parentage testing of Kilis goats reared in Turkey
    (TÜBİTAK, 2019) Keskin, Mahmut; Yılmaz, Onur; Gündüz, Zuhal; Ata, Nezih; Gül, Sabri; Cemal, İbrahim; Karaca, Orhan; Önel, Süleyman Ercüment
    The aim of this study was to develop PCR-based suitable microsatellite marker panels for paternity testing and to define pedigree errors in Kilis goats. A total of 137 head of goats were used, consisting of 118 head of kids and 19 head of possible candidate sires. A total of 392 alleles were observed in 22 microsatellite markers. Allele numbers ranged between 12 (SRCRSP7) and 24 (BM1818, INRA0023, and SRCRSP15) with an average of 17.82. The mean value of the effective allele numbers was obtained as 9.44. The overall polymorphic information content value was quite high (0.88). The overall observed (Ho) and expected heterozygosity (He) values for all studied loci were 0.89 and 0.89, respectively. Paternity test panels for the Kilis goat population studied were created based on individual probability of exclusion of microsatellites in multiplex groups. Combined probability of exclusion (CPE) values for different panels ranged between 0.745 (Panel-1) and 0.9999 (Panel-22), while the combined identification probability (CPI) values were obtained in the range of 9.81 × 10–3 (Panel-1) to 6.96 × 10–21 (Panel-22). As a result of this study, it can be stated that panels with 0.999 CPE values can be used at the most reasonable cost and with high reliability in paternity tests to be performed in Kilis goats, which can be a reference for other populations.