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    Adenosine deaminase and biochemical liver function tests in the dermatophytic cattle
    (Natl Veterinary Research Inst, 2006) Atakisi, Emine; Karapehlivan, Mahmut; Atakisl, Onur; Kontas, Tunay; Marasli, Saban
    Adenosine deaminase (ADA) activity and liver function tests were examined in dermatophytic cattle. In addition, the possible effect/s of the disease oil the activities of liver enzymes were investigated. Tell dermatophytic and 10 healthy animals aged between 10 and 12 months were used. Blood samples were obtained from each animal. The samples were left for 30 min at room temperature, and then centrifuged at 3000 x g for 10 min. Serum ADA, lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST). gammaglutamyl transferase (GGT) Were analysed colorimetrically. Serum ADA, LDH, and ALT activities were significantly higher in dermatophytic cattle, compared to those of healthy group (P < 0.01). ill addition. GGT and AST activities ill dermatophytic cattle were statistically compared higher to those of the healthy ones. Ill conclusion, the results of the present Study show that the increases in ADA and liver enzyme activities call be in indication of the loss of liver function. The increase in enzyme activity in the current study might have been caused by metabolic products of the Fungi.
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    Is CAPE a Therapeutic Agent for Wound Healing?
    (Medwell Online, 2009) Kontas-Askar, Tuenay; Altug, M. Enes; Karapehlivan, Mahmut; Atakisi, Emine; Hismiogullari, Adnan Adil
    Wound healing involves a number of processes, including an acute inflammatory response to production of Reactive Oxygen Species (ROS) and depression in antioxidant status. The aim of the study is to examine the therapeutic effects of Caffeic Acid Phenethyl Ester (CAPE), an antioxidant agent, on cutaneous wound healing in a rat model. There was a significant decrease in serum, wound and liver tissue TSA and serum LBSA levels in the CAPE group than in the control group. Also there was a significant decrease in serum and wound tissue TAC levels of the control group when compared to the CAPE group. But there was no difference between the groups in serum ADA activity, PBSA levels and liver tissue TAC levels. In conclusion, the present study showed the CAPE's efficacy in the treatment of cutaneous wounds by its antioxidant and ROS-scavenging capabilities.
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    Serum sialic acids, total antioxidant capacity, and adenosine deaminase activity in cattle with theileriosis and anaplasmosis
    (Natl Veterinary Research Inst, 2008) Guzel, Murat; Askar, Tunay Kontas; Kaya, Galip; Atakisi, Emine; Avci, Gulcan Erbil
    In the present study, we aimed to provide information on the serum content of sialic acids (TSA, LBSA, and PBSA), total antioxidant capacity (TAC), and adenosine deaminase (ADA) activity in cattle affected with naturally acquired theileriosis and anaplasmosis. A total of 55 Holstein cattle, comprising of 15 clinically healthy control animals, 20 cattle with theileriosis, and 20 with anaplasmosis, were used. Diagnosis was based on clinical signs, Giemsa stained blood or lymph node aspirate, and PCR assay. For the PCR assay, Tams I primers were used. The obtained results suggested that the concentration of sialic acids and ADA activity were significantly higher; and TAC were significantly lower in the theileriosis and anaplasmosis groups in contrast to the control group. In conclusion, the increased level of sialic acids and ADA in theileriosis and anaplasmosis may be attributable to the stimulation of the host immune response. In contrast, the reduced level of TAC may reflect a decrease in the antioxidant capacity.