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    Combination of cysteamine and lipoic acid improves the post-thawed bull sperm parameters
    (2016) Güngör, Şükrü; Aksoy, Adil; Yeni, Deniz; Avdatek, Fatih; Öztürk, Caner; Ataman, Mehmet Bozkurt; Coyan, Kenan; Bucak, Mustafa Numan; Başpınar, Nuri; Peker Akalın, Pınar
    The present study was conducted to examine the protective roles of cysteamine, trehalose, alpha-lipoic acid and combinations of these antioxidants on post-thawed bull sperm and oxidative stress parameters. Five healthy Holstein bull (3-4 years old) were used. Eight ejaculates for each bull were collected and pooled. Pooled ejaculate, splitted into seven equal aliquots and diluted at 37 °C with base extenders containing cysteamine 2 mM, trehalose 50 mM, alpha-lipoic acid (ALA) 1 mM, cysteamine 2 mM + trehalose 50 mM, ALA 1 mM + trehalose 50 mM, cysteamine 2 mM + ALA 1 mM and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The combination of cysteamine 2 mM and ALA 1 mM of the semen extender improved the percentages of post-thawed subjective motility (68 ± 2.7%), and progressive motility (42.9 ± 4.7%), compared with the controls (61 ± 4.2% and 37.5 ± 8%, respectively, non- significantly, P>0.05). The supplementation of the semen extender with combination of cysteamine 2 mM and ALA 1 mM produced a higher acrosome integrity and mitochondrial activity (52.02 ± 6.4% and 32 ± 4.1%, respectively), compared with the controls (30.5 ± 1.7 and 14.02 ± 3.5% respectively, P < 0.05). Combination of cysteamine and ALA antioxidants in semen extenders provided the benefit in terms of sperm motilities, acrosome integrity and mitochondrial activity on frozen-thawed bull sperm.
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    Prevention of embryonic death using different hormonal treatments in ewes
    (2013) Ataman, Mehmet Bozkurt; Aköz, Mehmet; Sarıbay, Mustafa Kemal; Erdem, Hüseyin; Bucak, Mustafa Numan
    The purpose of this study was to evaluate different treatment protocols to prevent embryonic death in ewes. A total of 180 Akkaraman crossbred ewes and 10 healthy rams were used as material. The ewes were divided into 3 equal groups, with each of the 3 groups then separated into 3 subgroups. Ewes in estrus, determined with teaser rams, were exposed to mating. Three different treatment protocols of gonadotropin-releasing hormone (GnRH) analog (buserelin, intramuscularly) at a dose of 20 &#956;g, vaginal sponges containing 30 mg fluorogestone acetate (FGA), and saline at a dose of 1 mL (control, intramuscularly) were applied on days 4, 12, and 16, respectively, for each subgroup after mating. No significant differences were observed in the pregnancy or multiple birth rates among any of the treatment groups. In the groups treated on days 4 and 12 after mating, the hormonal treatments gave lower rates of embryonic death compared to the control group (P < 0.05). In conclusion, the application of GnRH or FGA on days 4 and 12 after mating was found to be effective in preventing embryonic death in ewes.