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Öğe Antiperoxidative and anti-apoptotic effects of lycopene and ellagic acid on cyclophosphamide-induced testicular lipid peroxidation and apoptosis(Csiro Publishing, 2010) Turk, Gaffari; Ceribasi, Ali Osman; Sakin, Fatih; Sonmez, Mustafa; Atessahin, AhmetThe present study was conducted to investigate the possible protective effects of lycopene (LC) and ellagic acid (EA) on cyclophosphamide (CP)-induced testicular and spermatozoal toxicity associated with the oxidative stress and apoptosis in male rats. Forty-eight healthy adult male Sprague-Dawley rats were divided into six groups of eight rats each. The control group was treated with placebo; the LC, EA and CP groups were given LC (10 mg kg(-1)), EA (2 mg kg(-1)) and CP (15 mg kg(-1)), respectively, alone; the CP+LC group was treated with a combination of CP (15 mg kg(-1)) and LC (10 mg kg(-1)); and the CP+EA group was treated with a combination of CP (15 mg kg(-1)) and EA (2 mg kg(-1)). All treatments were maintained for 8 weeks. At the end of the treatment period, bodyweight and the weight of the reproductive organs, sperm concentration and motility, testicular tissue lipid peroxidation, anti-oxidant enzyme activity and apoptosis (i.e. Bax and Bcl-2 proteins) were determined. Administration of CP resulted in significant decreases in epididymal sperm concentration and motility and significant increases in malondialdehyde levels. Although CP significantly increased the number of Bax-positive (apoptotic) cells, it had no effect on the number of Bcl-2-positive (anti-apoptotic) cells compared with the control group. However, combined treatment of rats with LC or EA in addition to CP prevented the development of CP-induced lipid peroxidation and sperm and testicular damage. In conclusion, CP-induced lipid peroxidation leads to structural and functional damage, as well as apoptosis, in spermatogenic cells of rats. Both LC and EA protect against the development of these detrimental effects.Öğe Attenuating effect of lycopene and ellagic acid on 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced spermiotoxicity and testicular apoptosis(Taylor & Francis Ltd, 2011) Sonmez, Mustafa; Turk, Gaffari; Ceribasi, Ali Osman; Sakin, Fatih; Atessahin, AhmetThis study was conducted to investigate the prophylactic effects of lycopene (LC) and ellagic acid (EA) on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced testicular and spermatozoal toxicity. These toxicological changes are associated with the oxidative stress and apoptosis in male rats. Forty-eight male rats were allocated to one of six groups of 8 rats each: control, LC, EA, TCDD, TCDD+LC, and TCDD+EA. The control group was treated with 0.5 mL/rat slightly alkaline solution+0.5 mL/rat corn oil every other day. The LC group was treated with 0.5 mL/rat slightly alkaline solution+0.5 mL/rat corn oil containing 10 mg/kg of LC every other day. The EA group received 0.5 mL/rat corn oil+0.5 mL/rat slightly alkaline solution containing 2 mg/kg of EA every other day. The TCDD group received 0.5 mL/rat corn oil containing 100 ng/kg/day of TCDD+0.5 mL/rat slightly alkaline solution. The TCDD+LC group was treated with 0.5 mL/rat TCDD+0.5 mL/rat LC. The TCDD+ EA group was treated with 0.5 mL/rat TCDD+ 0.5 mL/rat EA. All treatments were made by gavage, and the experimental period was maintained during 8 weeks. Sperm motility, concentration, and abnormal sperm rate in epididymal tissue, testicular tissue lipid peroxidation (LPO), antioxidant enzyme activity, histopathological changes, and apoptosis (i.e., Bax and Bcl-2 proteins) were determined. TCDD exposure resulted in significant decreases in sperm motility, concentration, testicular superoxide dismutase activity, germinal cell-layer thickness, Johnsen's testicular score, and significant increases in abnormal sperm rate, testicular malondialdehyde, glutathione levels, Bax-positive staining, and Bax-positive apoptotic cell score, along with some testicular histopathological lesions. TCDD treatment did not affect significantly catalase activity. However, combined treatment with LC or EA, in addition to TCDD, prevented the development of TCDD-induced damages in sperm quality, testicular histology, and LPO. Improvements in testicular apoptosis after the administration of LC and EA to TCDD-treated rats were minimal, but not statistically significant. TCDD-induced lipid peroxidation leads to functional and structural damages, as well as apoptosis, in spermatogenic cells of rats. Both LC and EA protected against the development of these effects.Öğe The effect of raffinose and methionine on frozen/thawed Angora buck (Capra hircus ancryrensis) semen quality, lipid peroxidation and antioxidant enzyme activities(Academic Press Inc Elsevier Science, 2010) Tuncer, Puerhan Barbaros; Bucak, Mustafa Numan; Sariozkan, Serpil; Sakin, Fatih; Yeni, Deniz; Cigerci, Ibrahim Hakki; Atessahin, AhmetThe aim of the present study was to determine the effects of different doses of raffinose and methionine on post-thawed semen quality, lipid peroxidation and antioxidant enzyme activities of Angora buck (Capra hircus ancryrensis) sperm following cryopreservation. Ejaculates collected from three Angora bucks were evaluated and pooled at 37 degrees C. Semen samples, which were diluted with a Tris-based extender containing the additives raffinose (2.5, 5, 10 mM) and methionine (2.5, 5, 10 mM) and an extender containing no antioxidants (control), were cooled to 5 degrees C and frozen in 0.25 ml French straws. Frozen straws were thawed individually at 37 degrees C for 20 s in a water bath for evaluation. The freezing extender supplemented with 2.5 and 5 mM methionine led to higher percentages of CASA motility (63.6 +/- 7.0; 63.4 +/- 3.1%, respectively), in comparison to the controls (P < 0.01) following the freeze-thawing process. The addition of antioxidants did not provide any significant effect on the percentages of post-thaw subjective and CASA progressive motilities as well as sperm motion characteristics (VSL and VCL), compared to the control groups (P > 0.05). The freezing extender with raffinose (5 and 10 mM) and methionine at three different doses (2.5, 5 and 10 mM) led to lower percentages of acrosome abnormalities, in comparison to the controls (P < 0.001). In the comet test, raffinose (5 and 10 mM) and methionine (10 mM) gave scores lower than those of the controls, and thereby reduced DNA damage (P < 0.05). Malondialdehyde formation was found to be lower (1.8 +/- 0.1 nmol/L) in the group of 5 mM raffinose, compared to the controls following the freeze-thawing process (P < 0.01). The additives did not show any effectiveness on the maintenance of SOD, GSH-PX and GSH activities, when compared to the controls (P > 0.05). In conclusion, methionine and raffinose play a cryoprotective role against sperm CASA motility, acrosome abnormality and DNA damage. Raffinose 5 mM exhibited antioxidative properties, decreasing MDA levels. Further studies are required to obtain more concrete results on the characterization of microscopic parameters and antioxidant activities in cryopreserved goat sperm with different additives. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.Öğe Effects of 3-aminobenzamide on unilateral testicular ischemia-reperfusion injury: What is the role of PARP inhibition?(Canadian Science Publishing, 2010) Hekimoglu, Askin; Kurcer, Zehra; Aral, Faruk; Baba, Fusun; Atessahin, Ahmet; Sakin, FatihOn a examine les effets therapeutiques de l'inhibition de la poly(adenosine diphosphate-ribose) polymerase par le 3-aminobenzamide (3-AB) dans une lesion d'ischemie-reperfusion (I/R) des testicules en utilisant une analyse de sperme. On a effectue des examens histopathologiques et biochimiques pour mesurer les activites de la superoxyde dismutase (SOD), de la catalase (CAT), de la glutathion peroxydase (GSH-Px), et les taux de glutathion (GSH) reduit. On a divise des rats males en 3 groupes : ayant subi une operation factice (sham) (n = 12), I/R (n = 12) et I/R avec 3AB (I/R-3-AB) (n = 12). On a occlus l'artere testiculaire gauche pendant 1 h, puis on l'a soumis a une reperfusion pendant 24 h (pour les examens biochimiques et histopathologiques) et 30 jours (pour l'analyse de sperme). L'administration de 3-AB par voie intraperitoneale 10 min avant et 1 h apres la reperfusion a augmente la diminution induite par l'I/R de la motilite du sperme dans les 2 testicules, et elle a diminue l'augmentation anormale des taux de sperme dans le testicule ipsilateral. Toutefois, le traitement par 3-AB n'a pu prevenir la diminution induite par l'I/R du taux de sperme dans les 2 testicules. Les activites de SOD et de CAT sont demeurees stables chez tous les groupes. L'I/R a augmente l'activite de la GSH-Px et les taux de GSH. Le traitement par 3-AB a renverse l'augmentation induite par l'I/R de l'activite de la GSH-Px comme chez les sham, mais il n'a pas modifie les taux de GSH. Le traitement par 3-AB a augmente de maniere significative la diminution induite par l'I/R du score histopathologique. En conclusion, un traitement par 3-AB offre des avantages biochimiques et histopathologiques potentiels au-dela de la qualite du sperme et pourrait diminuer l'alteration de la torsion testiculaire.Öğe The Effects of Different Extenders and Myo-Inositol on Post-thaw Quality of Ram Semen(Kafkas Univ, Veteriner Fakultesi Dergisi, 2011) Kulaksiz, Recai; Bucak, Mustafa Numan; Akcay, Ergun; Sakin, Fatih; Daskin, Ali; Atessahin, AhmetThe study was conducted to evaluate the effects of different extenders and inositol additions on post-thaw semen quality, lipid peroxidation (LPO) and antioxidant activities. Semen was collected from four Karayaka rams from by artificial vagina three times a week. Semen samples showing normospermy quality were pooled. The pooled semen samples were extended in three extenders (Tris, T-, skimmed milk, M-and sodium citrate, NaC) with myo-inositol at two different doses (5 mM, 10 mM) and no antioxidant (control). Nine experimental groups were assigned as follows: T-5I, T-10I, T (control); M-5I, M-10I, M (control); Na-5I, Na-10I, NaC (control). Straws containing extended semen were equilibrated at 4 degrees C for 2 h, frozen in vapor of (15 min at -120 degrees C) liquid nitrogen and stored in liquid nitrogen. Frozen semen was thawed in a water bath at 37 degrees C for 30 seconds. The use of all the extenders supplemented with different doses of myo-inositol did not lead to any significant improvement in microscopic sperm and oxidative stress parameters (P > 0.05). Extenders of T and M resulted in higher sperm motility (50.00+/-2.24% and 55.00+/-0.42%) and HOST (49.00+/-3.32% and 48.17+/-2.97%) rates, compared to NaC (37.00+/-3.74% and 31.80+/-2.96%, P < 0.01), following the freeze/thawing process. Extenders supplementated with myo-inositol not significantly affect malondialdehyde (MDA) levels and activities of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-PX) in comparison to the control groups (P > 0.05), except for MDA level of T extender containing 10 mM inositol. MDA level was found lower (1.22+/-0.07 nmol/ml) in T than those of the M and NaC (P < 0.05). For GSH and GSH-PX activities, T and NaC gave the higher values, compared to M, following the freeze/thawing process (P < 0.01).Öğe Impact of ellagic acid on adriamycin-induced testicular histopathological lesions, apoptosis, lipid peroxidation and sperm damages(Elsevier Gmbh, Urban & Fischer Verlag, 2012) Ceribasi, Ali Osman; Sakin, Fatih; Turk, Gaffari; Sonmez, Mustafa; Atessahin, AhmetThe aim of the present study was to investigate whether ellagic acid (EA) has protective effect on adriamycin (ADR)-induced testicular and spermatozoal toxicity associated with the oxidative stress in male rats. Thirthy-two healthy 8-week-old male Sprague-Dawley rats were equally divided into four groups. The first (EA) group was treated with EA (2 mg/kg/every other day) by gavage. The second (ADR) group received ADR (2 mg/kg/once a week) intraperitoneally, while the combination of ADR and EA was given to the third (ADR+EA) group. The forth (control) group was treated with placebo. At the end of the 8-week treatment period, reproductive organ weights, epididymal sperm parameters, histopathological changes and apoptosis via Bax and Bcl-2 proteins, testicular tissue lipid peroxidation, and antioxidant enzyme activities, were investigated. ADR administration was determined to cause significant decreases in reproductive organ weights, epididymal sperm concentration and motility, plasma testosterone concentration, diameter of seminiferous tubules, germinal cell layer thickness Johnsen's testicular score and Bcl-2 positive antiapoptotic cell rate, wherease it caused significant increases in level of lipid peroxidation and glutathione, catalase activity, abnormal sperm rates and Bax positive apoptotic cell rates along with degeneration, necrosis, immature germ cells, congestion and atrophy in testicular tissue when compared with the control group. EA administration to ADR-treated rats provided significant improvements in ADR-induced disturbed oxidant/antioxidant balance, decreased testosterone concentration, testicular apoptosis and mild improvements in the histopathological view of the testicular tissue. However. EA failed to improve decreased reproductive organ weights and deteriorated sperm parameters due to ADR administration. It is concluded that while ADR has direct or indirect (lipid peroxidation) negative effects on sperm structure and testicular apoptosis in rats, EA has protective effects on ADR-induced testicular lipid peroxidation and apoptosis. (C) 2011 Elsevier GmbH. All rights reserved.Öğe Phenotyping Determination of in vivo CYP2D6 Enzyme Activity Used as A Probe Debrisoquine in Swiss Black, Holstein, Simmental and Eastern Anatolian Red Cow Breeds(Kafkas Univ, Veteriner Fakultesi Dergisi, 2013) Sakin, Fatih; Baydar, Ersoy; Servi, Kadir; Atessahin, Ahmet; Dagoglu, GurdalIn the current study was carried out to determine phenotyping of in vivo CYP2D6 enzyme activity used as a probe debrisoquine (DEB) in Swiss Black (SB), Holstein (HOL), Simmental (SI) and Eastern Anatolian Red (EAR) cows. In the study, totally 60 cows, fifteen cows from each breed, were used. DEB was application at 0.5 mg/kg. Urine samples were collected throughout 12. and 24th h after DEB application. The metabolic (DMR) and recovery (DRR) rates of DEB in urine samples were calculated to evaluate the in vivo activity of CYP2D6 enzyme activity. The DMR value of EAR at 12th h were significantly higher than those others (P<0.01) while this value significantly lower in EAR compared to that of others (P<0.01). The phenotyping CYP2D6 enzyme activity of EAR at 12th h was considered as poor metaboliser (PM) while this phenotype was extensive metabolizer (EM) in SB, HOL and SI cows. In conclusion, it can be considered that urine samples taken at 12th h after DEB administration at dose of 0.5 mg/kg as probe can be used to determine in vivo phenotyping of CYP2D6 enzyme activity in cows. Besides, in implementation of treatment with drugs that are substrates of CYP2D6 in cows, it can be concluded that these drugs will be metabolized more slowly, and the duration time of body and effect durations of action will be increased in EAR cows.Öğe Protective effects of conventional and colon-targeted lycopene and linalool on ulcerative colitis induced by acetic acid in rats(Springer Basel Ag, 2019) Tekeli, Ibrahim Ozan; Atessahin, Ahmet; Sakin, Fatih; Aslan, Abdullah; Ceribasi, Songul; Yipel, MustafaObjectiveTo compare the potential protective effects of conventional and colon-targeted lycopene (TLC) and linalool (TLN) on acetic acid (AA)-induced ulcerative colitis (UC) in rats.MethodsConventional and colon-targeted LC (10mg/kg) and LN (200mg/kg) were administered in vivo orally for 7days and sulfasalazine (100mg/kg) was also used as reference drug. Then, 4% AA was administered intrarectally to induce UC. Subsequently, the colon tissues were taken as samples for biochemical and histopathological analysis.ResultsMalondialdehyde (MDA), interleukin 1 (IL-1), IL-6, cyclooxygenase-2 (COX-2) and nuclear factor kappa B (NF-B) levels were decreased (p<0.05) in the targeted groups compared to the AA group, whereas nuclear factor-erythroid 2-related factor 2 (Nrf-2) level was increased (p<0.05). Tumor necrosis factor (TNF-) level was also decreased (p<0.05) and catalase activity (CAT) was increased (p<0.05) in the TLC group compared to the AA group. IL-1 and IL-6 levels were lower in the TLC group compared to the conventional LC and sulfasalazine groups (p<0.05). COX-2 and NF-B levels were lower, while the Nrf-2 level was higher in the targeted groups compared to the conventional groups (p<0.05). Furthermore, COX-2 level was lower and Nrf-2 level was higher in the targeted groups compared to the sulfasalazine group (p<0.05).ConclusionAs expected, sulfasalazine was effective on all parameters analyzed, but the colon-targeted pretreatments were more effective from sulfasalazine on some parameters. Therefore, colon-targeted plant-derived therapies might be alternative approaches to provide protection against UC, which deserves to be investigated further.
