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  • Küçük Resim
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    Apoptosis and cell proliferation in short-term and long-term effects of radioiodine-131-induced kidney damage: an experimental and immunohistochemical study
    (Lippincott Williams & Wilkins, 2018) Yumusak, Nihat; Sadic, Murat; Yucel, Gozde; Atilgan, Hasan I.; Koca, Gokhan; Korkmaz, Meliha
    ObjectiveRadioiodine-131 is a radionuclide that is used for therapeutic purposes in hyperthyroidism and thyroid cancer. The aim of this study was to evaluate apoptotosis and proliferative changes in radioiodine-related kidney damage.Materials and methodsThree groups (n=10/group) of rats were used as follows: the rats were in group 1 untreated, and the rats in groups 2 and 3 were treated once with oral radioiodine (111MBq). The animals in group 2 were killed at the end of the seventh day and the rats in group 3 were killed at the end of the 10th week. The kidneys were removed and evaluated immunohistochemically. The presence of radioiodine in the kidneys was shown by the Na+/I-symporter antibody and proliferating cell nuclear antigen, Ki-67, caspase-3, caspase-8, caspase-9, and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling assay were used to detect cell proliferation and apoptosis.ResultsNa+/I-symporter protein accumulation in the kidneys was observed to be significantly greater in group 2 than in group 3 (P<0.05). All the immunohistochemical analyses showed that cell proliferation and apoptosis began on the seventh day and peaked in the 10th week. The proliferating cell nuclear antigen, Ki-67, and caspase expressions and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling values were all found to be statistically significantly increased in group 3 compared with the other groups (P<0.05).ConclusionRadioiodine caused cell proliferation and apoptosis as shown by immunohistochemistry.
  • Küçük Resim
    Öğe
    The scintigraphic, biodistribution and histopathological evaluation of the effect of experimental 131I administration on the gastrointestinal system and the demonstration of the Na+/I- symporter by immunohistochemistry in rats
    (Lippincott Williams & Wilkins, 2018) Yilmaz, Rahsan; Yumusak, Nihat; Sadic, Murat; Atilgan, Hasan I.; Korkmaz, Meliha; Bozkaya, Faruk
    ObjectiveThe aim of this study was to investigate histopathological changes and biodistribution of iodine-131 (I-131) in the gastrointestinal system (GIS) and also Na+/I- Symporter (NIS) presence by immunohistochemically in the experimental treatment of rats with radioactive iodine (RAI).Materials and methodsRats were divided into experimental and control groups as random early group 2 (24h), intermediate group 3 (3 weeks), and late period group 4 (3 months). Experimental groups were administered 100MBq (approximate to 3mCi, 12mCi/kg) by orogastric route with orogastric tube. Scintigraphic iodine screening images were obtained 24h, 3 weeks, and 3 months after RAI, and GIS tissues were removed, and immunohistochemical methods were used to demonstrate NIS with RAI biodistribution and histopathology.ResultsAccording to the results of scintigraphy, the most prominent activity involvement was observed in the thyroid gland at group 2, and significant activity was observed in the stomach. In the group 3 and group 4 images, owing to the physiological and biological half-life of the iodine and low resolution of the gamma camera, no secondary focal activation was observed. The highest RAI biodistribution value in all groups was in the stomach, ileum and oesophagus. In the immunohistochemical examination of NIS, the highest staining sequence was observed in all groups respectively in the stomach, oesophagus, tongue, colon, saliva, duodenum, rectum, ileum and jejunum. The increase of NIS immunohistochemically stained more intensely was observed in the RAI-administered groups.ConclusionThe amount of NIS is important for the absorption of RAI after administration.