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    The architecture of the lymph nodes in the abdominal and thoracic cavities of wild boar
    (Agricultural Research Communication Centre, 2019) Bozkurt, Yesim Akaydin; Ates, Sevinc; Kozlu, Tolunay; Basak, Feyza
    The distrubition of lymph nodes located in the abdominal and thoracic cavities of ten wild boars, and their structure were determined anatomically, histologically and immunohistochemically, to be the first detailed investigation on the wild boar. Though general localization and distribution were similar, the number of lymph nodes showed small differences from those of domestic pig. Histological investigations did not reveal a significant hilus. Besides, T lymphocytes with anti-CD3, CD4, CD8, B lymphocytes with anti-CD79a, macrophages with anti-macrophage monoclonal antibodies, and follicular dendritic cells using anti-S100 polyclonal antibody and their distrubution in the lymph nodes were detected. Many CD3 positive T lymphocytes were observed in the germinal center of the lymph follicles, in the cortical area and in the medulla. CD8 positive T lymphocytes were few, and CD4 positive T lymphocytes were not seen. CD79 positive cells were scanty.
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    Determination of some of the lectins' immunohistochemistry profile of the dog testis and epididymis
    (Tubitak Scientific & Technological Research Council Turkey, 2022) Basak, Feyza; Akaydin Bozkurt, Yesim; Dabrowski, Roman
    Lectins are substances composed of proteins or glycoproteins that specifically bond to carbohydrates on the cell surfaces or inside the cell. Lectins are used to identify the carbohydrate types in the cellular membrane or cytoplasm. The aim of the study was to identify the location and distribution of the four types of lectins PNA (Peanut agglutinin- Arachis hypogea), WGA (Wheatgerm Agglutinin- Triticum vulgaris), Con A (Conconavalin A-Canavalia ensiformis), and SBA (Soybean Agglutinin-Glycine max) in dog testes and epididymites. The testes and the body of 10 adult dogs were collected from an animal shelter after castration. Following the standard histological procedures, lectin histochemistry was conducted on paraffin sections. In epididymal ducts and seminiferous tubules, the reaction was positive for PNA, WGA, and SBA. The deferent duct reaction was positive in all types of lectins. Based on the study data, we can suggest that the analysis of the results could be beneficial for cell and tissue culture techniques, as well as stem cell research, sperm maturation, canine capacitation, and decapacitation.