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Öğe Characterization of normal and mastitic milks using Raman Spectroscopy by application of SERS method(Hellenic Veterinary Medical Soc, 2024) Acikgoz, G.; Coskun, P.; Ergun, Y.; Baspinar, N.; Kazak, F.Rapid, non-destructive analysis of the milk by Raman Spectroscopy is used in different analyses and has enabled the determination of biological samples without using additional chemicals. In the study, it is aimed to investigate the molecular structures of normal and mastitic milks using Raman spectroscopy by application of Surface Enhanced Raman Spectroscopy (SERS) method and this study is the first study done for this purpose. In the study, the presence or absence of functional groups of the spectra obtained from the milk samples was compared with the reference spectra in other studies. It was observed that the peaks obtained from the biochemical structures of normal and mastitic milks were consistent with the literature. In addition, it was found that the biochemical components (such as fat, proteins, carbohydrates and minerals) of subclinical mastitis and normal milk did not change at the molecular level. It was thought that there was no change at the molecular level as there was no visible deterioration in the milk with subclinical mastitis and the factor caused by the infection in the breast tissue.Öğe A Comparative Study of Seminal Plasma and Blood Serum Macro and Trace Elements in the the Breeding (October) and the Non-Breeding (April) Seasons in Merino Ram(Hellenic Veterinary Medical Soc, 2019) Bulbul, B.; Akalin, P. P.; Baspinar, N.; Bucak, M. N.; Kirbas, M.; Ozturk, C.; Gungor, S.In this study, it was aimed to investigate the concentrations of macro and trace elements in seminal plasma and blood serum in the breeding (October) and the non-breeding (April) seasons in Merino Ram. Nineteen Merino Rams, aged 18-24 months, were involved in the study. Blood (once) and ejaculate samples (6 replicates) were taken in the breeding (October) and the non-breeding (April) seasons. Blood serum, seminal plasma and diet Calcium, Sodium, Potassium, Magnesium, Phosphorus, Sulfur, Zinc, Selenium, Chrome, Manganese, Nickel, Molybdenum and Boron concentrations were determined by ICP-AES. In blood serum, Sodium and Selenium concentrations were higher (p<0.05 and 0.001, respectively) in the the breeding season than in non-breeding season, whereas Potassium, Chromium and Boron concentrations were lower (p<0.05, 0.001 and 0.001, respectively) in the breeding season than in the non-breeding season. In seminal plasma Calcium, Sodium, Zinc and Manganese concentrations were higher (p<0.05, 0.001, 0.01 and 0.05, respectively) in the breeding season than in the non-breeding season, whereas Phosphorus, Chrome, Molybdenum and Boron concentrations were lower (p<0.001, 0.001, 0.05 and 0.001, respectively) in the breeding season than in the non-breeding season. No difference was detected regarding the other elements. The higher levels of Cr and B in the non-breeding season compared to the breeding season both in serum and seminal plasma, regardless of diet intake, suggest that these elements may play a crutial role on male fertility in Merino Ram.Öğe Effects of boron administration on hepatic steatosis, hematological and biochemical profiles in obese rabbits(Dustri-Verlag Dr. Karl Feistle, 2010) Basoglu, A.; Baspinar, N.; Sagkan Ozturk, A.; Peker Akalin, P.Objective: This study was designed to determinate the short and long term effects of boron at different doses on hematological and biochemical profiles, liver histology, and to evaluate boron efficacy on the pathogenic mechanisms leading to fatty liver. The aim of this study is to provide insight into boron metabolism. Material and methods: Boron was orally administrated in rabbits fed high energy diet at three different doses and 96 h, intervals for 7 months. Liver histology, and hematological, biochemical and ICP-AES analysis were performed. Results: Complete blood count and acid-base balance were not affected by short and long term boron administration. Decreases in hepatic fat infiltration and visceral fat amount in spite of body weight gain were considerable. The most pronounced findings were significant changes in hepatic TAG concentration and antioxidant status, and lipid profile. High boron doses have been tolerated at 96 h intervals. Conclusion: High boron doses can be tolerated for a long time at 96 h intervals. Boron seems to be effective on hepatic and visceral fat accumulation. This may be attributed to its effects of improving energy status, lowering oxidative stress and affecting lipid profile. ©2010 Dustri-Verlag Dr. K. Feistle.Öğe Effects of boron on serum and liver macro elements in rabbits fed with high energy diet(Wiley-Blackwell, 2012) Akalin, P. P.; Baspinar, N.; Basoglu, A.; Sagkan, A.[Abstract Not Available]Öğe Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate in Merino ram(Wiley, 2015) Akalin, P. P.; Akalin, P. Peker; Baspinar, N.; Coyan, K.; Bucak, M. N.; Gungor, U.; Ozturk, C.[Abstract Not Available]Öğe Glucose-6-phosphate dehydrogenase, glutathione peroxidase, total glutatione and reduced nicotinamide adenine dinucleotide phosphate in milk cells of subclinical mastitic cows(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2019) Akalin, P. P.; Ergun, Y.; Baspinar, N.; Dogruer, G.; Kucukgul, A.; Cantekin, Z.; Isgor, M.This study aimed to determine the levels of milk cell total protein (TP), reduced nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), activities of glucose-6-phosphate dehydrogenase (G6PD) and glutathione peroxidase (GPx) in subclinical mastitic cows. Milk from each udder was collected and grouped by the California Mastitis Test. Then, a somatic cell count (SCC) was performed, and the groups were re-scored as control (5-87 x 10(3) cells), 1st group (154-381 x 10(3) cells), 2nd group (418-851 x 10(3) cells), 3rd group (914-1958 x 10(3) cells), and 4th group (2275-8528 x 10(3) cells). Milk cell TP, NADPH, tGSH levels, G6PD, and GPx activities were assessed. Microbiological diagnosis and aerobic mesophyle general organism (AMG, cfu/g) were also conducted. In mastitic milk, TP, NADPH, and tGSH levels, and G6PD and GPx activities were significantly reduced per cell (in samples of 10(6) cells). In addition, milk SCC was positively correlated with AMG (r=0.561, p<0.001), NADPH (r=0.380, p<0.01), TP (r=0.347, p<0.01) and G6PD (r=0.540, p<0.001). There was also positive correlation between NADPH (r=0.428, p<0.01), TP (r=0.638, p<0.001) and AMG. NADPH was positively correlated with TP (r=0.239, p<0.05), GPx (r=0.265, p<0.05) and G6PD (r=0.248, p=0.056). Total protein was positively correlated with tGSH (r=0.354, p<0.01) and G6PD (r=0.643, p<0.001). There was a negative correlation between tGSH and GPx activity (r=-0.306, p<0.05). The microbiological analysis showed the following ratio of pathogens: Coagulase-Negative Staphylococci 66.6%, Streptococcus spp 9.5%, Bacillus spp 9.5%, yeast 4.8%, and mixed infections 9.5%. As a conclusion, when evaluating the enzyme and oxidative stress parameters in milk, it is more suitable to assign values based on cell count rather than ml of milk. The linear correlation between the SCC and AMG, milk cell NADPH, TP and G6PD suggests that these parameters could be used as markers of mastitis.Öğe Relationships between glucose-6-phosphate dehydrogenase, glutathione peroxidase, reduced nicotinamide adenine dinucleotide phosphate, total protein, malondialdehyde, total glutathione and vitamin C parameters in goat milkcells(Hellenic Veterinary Medical Soc, 2022) Kazak, F.; Karafakioglu, Y.; Baspinar, N.; Coskun, P.In this study, to reveal the antioxidant potential of goat milk cells, the activities of glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx) and the levels of reduced nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), malondialdehyde (MDA), vitamin C (Vit C) and total protein (TP) in goat milk cells were determined and correlations between these parameters were evaluated. Milk samples were collected from 19 clinically healthy goats from a private goat farm. Briefly, milk cells were collected from milk by centrifugation and than they were sonicated. Supernatant G6PD, GPx activities and NADPH, tGSH, MDA, Vit C and total protein levels were determined by spectrophotometric methods. As regards correlations: milk cell MDA levels were positively correlated with milk cell tGSH (r=0, 725, p<0, 01), milk cell Vit C (r=0.622, P<0.01) and milk cell NADPH (r=0.763, P<0.01) levels. There was a positive correlation between milk cell GPx activity and milk cell NADPH levels (r=0.659, P<0.01). Milk cell tGSH levels were positively correlated with milk cell Vit C (r=0.615, P<0.05) and milk cell NADPH (r=0.846, P<0.01) levels. Milk cell NADPH levels was positively correlated with milk cell Vit C levels (r=0.791, P<0.01). As a conclusion, the antioxidant potential of goat milk cells were evaluated and discusses.
