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Öğe Effects of Long-Term Boron Administrations on High-Energy Diet-Induced Obesity in Rabbits: NMR-Based Metabonomic Evaluation(Medwell Online, 2011) Basoglu, Abdullah; Baspinar, Nuri; Ozturk, Aliye Sagkan; Akalin, Pinar PekerThe aim of this study is to provide insight into boron metabolism and to identify metabolic pathways which may explain the presumed increased susceptibility of livers. Boron was administrated in rabbits at three different doses and 96 h intervals for 7 months. Metabolomic profile based on NMR analysis was performed. The most pronounced findings were significant changes in alanine, methionine, pyruvate and creatine. Boron seems to be effective in the prevention of obesity and fatty liver. Metabolic end-points obtained by NMR can be easily assessed and interpreted alone or in. combination each other and with classical biochemical parameters for better understanding obesity and boron and liver metabolism.Öğe Effects of long-term Boron administrations on high-energy diet-induced obesity in rabbits: NMR-based metabonomic evaluation(2011) Basoglu, Abdullah; Baspinar, Nuri; Ozturk, Aliye Sagkan; Akalin, Pinar PekerThe aim of this study is to provide insight into boron metabolism and to identify metabolic pathways which may explain the presumed increased susceptibility of livers. Boron was administrated in rabbits at three different doses and 96 h intervals for 7 months. Metabolomic profile based on NMR analysis was performed. The most pronounced findings were significant changes in alanine, methionine, pyruvate and creatine. Boron seems to be effective in the prevention of obesity and fatty liver. Metabolic end-points obtained by NMR can be easily assessed and interpreted alone or in combination each other and with classical biochemical parameters for better understanding obesity and boron and liver metabolism. © Medwell Journals, 2011.Öğe Erythrocyte and spermatozoa glucose-6-nhosphate dehydrogenase activity in merino rams: An experimental study(Shahid Sadoughi Univ Medical Sciences, 2018) Gurel, Huseyin; Baspinar, Nuri; Akalin, Pinar Peker; Altunok, Vahdettin; Kazak, FilizBackground: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate metabolic pathway that supplies reducing agents by maintaining the level of reduced nicotinamide adenine dinucleotide phosphate. Objective: It was aimed to determine the activity of erythrocyte and spermatozoa G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find out the relation of these parameters with sperm quality parameters for better understanding the role of this enzyme in male fertility. Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved. Ejaculate samples were collected using an artificial vagina, in October (the breeding season) and April (the non-breeding season). Blood samples were collected prior to sperm collection. Sperm volume (ml), motility (%), mass activity (1-5), concentration (x10(6)), viability (%), abnormal acrosome morphology (%) and abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and erythrocyte G6PD were determined and the relation of sperm parameters with G6PD activity was evaluated. Results: Erythrocyte G6PD activity was higher (p <= 0.001), whereas spermatozoa G6PD activity was lower (p<0.001) in the breeding season (1.928 +/- 0.231 U/g hemoglobin, 129.65 +/- 28.41 U/g protein, respectively) from that in the non-breeding (0.530 +/- 0.066 U/g hemoglobin, 562.36 +/- 94.92 U/g protein, respectively). There were also significant differences among sperm quality parameters within the seasons. Positive correlation was determined between spermatozoa G6PD activity (r=0.053, p=0.03 and sperm concentration in the breeding season. Conclusion: Higher spermatozoa G6PD activity in October, where the level of polyunsaturated fatty acids is suggested to be increased, may reflect the increased need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity for the oxidative balance.Öğe Erythrocyte and spermatozoa glucose-6-phosphate dehydrogenase activity in merino rams: An experimental study(Research and Clinical Center for Infertitlity, 2018) Gurel, Hüseyin; Baspinar, Nuri; Akalin, Pınar Peker; Altunok, Vahdettin; Kazak, FilizBackground: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate metabolic pathway that supplies reducing agents by maintaining the level of reduced nicotinamide adenine dinucleotide phosphate. Objective: It was aimed to determine the activity of erythrocyte and spermatozoa G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find out the relation of these parameters with sperm quality parameters for better understanding the role of this enzyme in male fertility. Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved. Ejaculate samples were collected using an artificial vagina, in October (the breeding season) and April (the non-breeding season). Blood samples were collected prior to sperm collection. Sperm volume (ml), motility (%), mass activity (1-5), concentration (×106), viability (%), abnormal acrosome morphology (%) and abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and erythrocyte G6PD were determined and the relation of sperm parameters with G6PD activity was evaluated. Results: Erythrocyte G6PD activity was higher (p?0.001), whereas spermatozoa G6PD activity was lower (p?0.001) in the breeding season (1.928±0.231 U/g hemoglobin, 129.65±28.41 U/g protein, respectively) from that in the non-breeding (0.530±0.066 U/g hemoglobin, 562.36±94.92 U/g protein, respectively). There were also significant differences among sperm quality parameters within the seasons. Positive correlation was determined between spermatozoa G6PD activity (r=0.053, p=0.03 and sperm concentration in the breeding season. Conclusion: Higher spermatozoa G6PD activity in October, where the level of polyunsaturated fatty acids is suggested to be increased, may reflect the increased need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity for the oxidative balance. © 2018, Research and Clinical Center for Infertitlity. All rights reserved.Öğe Influence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5°C(Elsevier Science Bv, 2016) Peker Akalin, Pinar; Bucak, Mustafa Numan; Gungor, Sukru; Baspinar, Nuri; Coyan, Kenan; Dursun, Sukru; Ili, PinarEjaculates were collected from six Merino rams with the aid of an artificial vagina twice a week. The ejaculates containing spermatozoa with >80% forward progressive motility and concentrations higher than 2 x 10(6) spermatozoa/ml were pooled. The present study included two experiments. In experiment 1, each pooled ejaculate was divided into four equal aliquots and diluted (37 degrees C) with the Tris based extender, containing 0 (control), 0.5, 1 and 2 mM lycopene, at a final concentration of approximately 400 x 10(6) sperms/ml (single step dilution), In experiment 2, cysteamine at concentrations of 0 (control), 0.5,1 and 2 mM, was used as an additive in the extender, and the procedure explained above was applied for the division of aliquots and the dilution of semen. Diluted semen samples were kept in glass tubes and cooled from 37 degrees to 5 degrees C in a cold cabinet, and maintained at 5 degrees C. Sperm and oxidative stress parameters were evaluated after 0, 24, 48 and 72 h of storage at 5 degrees C. The extender supplemented with 0.5 mM lycopene resulted in higher mitochondrial activity rate (p<0.05) in comparison to the control group at 72 h of storage. Lycopene at 0.5 mM dose led to higher sperm motility rate (p<0.05) when compared to 2 mM lycopene group at 72 h of liquid storage. As regards oxidative stress parameters, only 2 mM lycopene increased total glutathione levels (p<0.05) at 0 h of storage. The extender supplemented with 1 mM cysteamine gave higher motility (p<0.05) at 48 h compared to control. As regards oxidative stress parameters, 1 and 2 mM cysteamine at 48 h and 1 mM cysteamine at 72 h increased total glutathione levels (p<0.05) compared to control groups. Cysteamine at 1 and 2 mM doses decreased lipid peroxidation (p<0.05) at 0 h of liquid storage compared to control. Our data suggest that lycopene at 0.5 and 2 mM and cysteamine at 1 and 2 mM doses can be added to Tris based extender for improving the ram sperm motility, viability, mitochondrial activity and oxidative stress parameters during the liquid storage. (C) 2016 Elsevier B.V. All rights reserved.Öğe Melatonin Levels After Heat Treatment in Goat Milk: Relation of Total Protein(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Ispir, Medine; Peker Akalin, Pinar; Kazak, Filiz; Baspinar, NuriObjective of this study was to investigate the effects of pasteurization (15 seconds at 72 degrees C) and boiling processes (5 minutes at 100 degrees C) on goat milk melatonin and total protein levels. The milk of 20 healthy goats obtained from a private goat farm were used as materials. Each milk sample was divided into 3 for raw milk. for pasteurization and for boiling process. Melatonin levels in raw. pasteurized and boiled milk were determined with commercial ELISA kit. while total protein levels were determined spectrophotometricly by manual methods. Melatonin levels in boiled milk samples (4.20 +/- 0.39 pg ml(-1)) increased (P<0.05) compared to raw milk (3.19 +/- 0.25 pg ml(-1)) samples. While there was no difference between the heat-treated groups in terms of total protein levels determined by Bradford method. the total protein levels determined by Lowry method decreased significantly in boiling process group compared to the other groups (P<0.001). Positive correlations were determined between total protein levels determined by Bradford and Lowry methods in raw (r=0.723, P<0.01.), pasteurized (r=0.838, P<0.01) and boiled (r=0.149, P<0.05) milk samples. Pasteurization process applied to goat- milk did not change milk melatonin and total protein levels, and boiling process decreased total protein levels, while increased melatonin levels. Before melatonin analysis was performed in milk by ELISA method, it was predicted that a pre-treatment of milk may be useful in order to separate melatonin from the bound protein.Öğe Protective and therapeutic effects of nobiletin against cisplatin-induced nephrotoxicity in rats(Taylor & Francis Ltd, 2024) Kazak, Filiz; Coskun, Pinar; Yarim, Gul Fatma; Baspinar, Nuri; Ozdemir, Ozgur; Ates, Mehmet Burak; Altug, Muhammed EnesPossible protective and therapeutic effects of nobiletin on kidney in a cisplatin-induced nephrotoxicity rat model were investigated. Forty male albino rats were divided into four groups: control, cisplatin (CIS), cisplatin+nobiletin (CIS+NOB), and nobiletin+cisplatin (NOB+CIS). At the end of the study, the rats were subjected to biochemical, histological and immunohistochemical analyzes. Compared to the control group, tGSH (p < 0.05) levels, and G6PD (p < 0.05) and GPx (p < 0.001) activities, were increased in the CIS group; while significant (p < 0.05) decreases occurred in the MDA and TOC levels. Histopathologically, the kidneys of the groups administered nobiletin (CIS+NOB, NOB+CIS) were significantly different from the CIS group, being closer to control group in terms of degeneration and hyaline cylinder formation in the tubules (p < 0.05). While dilatation in the tubules, protein-rich fluid and hyaline cylinder formation in the lumen were most common in the CIS group, a significant decrease (p < 0.05) of these parameters was seen in the nobiletin groups (CIS+NOB, NOB+CIS). This study suggests that nobiletin can be effective in preventing and ameliorating toxic effects of cisplatin on the kidney.Öğe Protective effects of nobiletin on cisplatin induced neurotoxicity in rats(Taylor & Francis Ltd, 2022) Kazak, Filiz; Akalin, Pinar Peker; Yarim, Gul Fatma; Baspinar, Nuri; Ozdemir, Ozgur; Ates, Mehmet Burak; Altug, Muhammed EnesObjectives This study was designed to investigate the possible antioxidant, antiapoptotic and neuroprotective effects of nobiletin on cisplatin-induced neurotoxicity rat model by evaluating neurotrophins, antioxidants and histopathology. Methods Forty male Wistar Albino rats were divided into four groups: control, cisplatin (CIS), cisplatin + nobiletin (CIS + NOB) and nobiletin + cisplatin (NOB + CIS). CIS + NOB was applied nobiletin (10 mg/kg, i.p.) during the last four days whereas NOB + CIS was applied nobiletin during the first four days of the study. Cisplatin (4 mg/kg, i.p. twice a day) was administered to the experimental groups on the 5th day of the study. All rats were sacrificed on the 10th day of the study. BDNF, NGF, G6PD, GPx, tGSH and MDA levels were determined in brain. In addition, routin histolopathological analysis and caspase-3 immunoreactivity assay were conducted. Results BDNF concentrations increased in nobiletin-administered groups, compared to Control and CIS and that the increase was statistically significant in NOB + CIS (p < 0.05). It was also found that G6PD activity increased (p < 0.05) in the nobiletin-administered groups, compared to control and CIS. Histopathologically, neuronal degeneration, oedema and gliosis increased in CIS compared to Control, and nobiletin administration decreased neuronal degeneration and oedema compared to CIS (p < 0.05). Cisplatin increased (p < 0.05) caspase-3 immunoreactivity in cerebrovascular endothelium and neurons compared to Control, while nobiletin administration decreased caspase-3 immunoreactivity in cerebrovascular endothelium. Caspase-3 immunoreactivity in neurons decreased only in NOB + CIS (p < 0.05). Conclusion Nobiletin increased BDNF concentration and G6PD activity in brain and when evaluated together with histopathological and immunohistochemical findings, it may have antioxidant, antiapoptotic and neuroprotective effects against cisplatin.Öğe Relationship of blood and seminal plasma ceruloplasmin, copper, iron and cadmium concentrations with sperm quality in Merino rams(Elsevier Science Bv, 2015) Akalin, Pinar Peker; Bulbul, Bulent; Coyan, Kenan; Baspinar, Nuri; Kirbas, Mesut; Bucak, Mustafa Numan; Gungor, SukruThe aim of the current study was to investigate the concentrations of ceruloplasmin, copper, iron, zinc and cadmium concentrations in blood serum and seminal plasma obtained from Merino rams. In addition, their relationship with sperm parameters, fertility rate and litter size were also studied. Blood and ejaculate samples (6 replicates) were taken in October from 19 Merino rams, aged between 18 and 24 months. Ceruloplasmin, copper, iron, zinc and cadmium in blood serum and seminal plasma were determined. Sperm parameters including volume, mass motility, motility, concentration, Hos-test, viability, abnormal sperm and acrosome abnormality in semen, fertility rate and litter size were also evaluated. Highly positive correlation was found between blood ceruloplasmin and blood copper concentrations (r=0.812, p<0.001), whereas negative correlation were determined between these parameters in seminal plasma (r=0.195, p<0.05). Seminal plasma copper concentration was positively correlated with seminal plasma cadmium (r=0.206, p<0.05) and seminal plasma iron (r=0,305, p<0.01) concentrations. Negative correlation was determined between blood ceruloplasmin level and acrosomal defect (r=0.443, p<0.05). Seminal plasma ceruloplasmin level was positively correlated with volume (r=0.255, p<0.01) and negatively correlated with abnormal sperm (r=0.186, p=0.058) and acrosome abnormality (r=0.213, p<0.05). Seminal plasma iron concentration was positively correlated with other abnormality (r=0.257, p<0.01). Seminal plasma cadmium concentration was positively correlated with sperm abnormality (r=0.207,p=0.052) and other abnormality (r=0.262,p <0.05) and negatively correlated with fertility rate (r=0.449,p =0.054). Blood cadmium concentration was negatively correlated with litter size (r=0.579, 9<0.01). In conclusion, blood and seminal plasma ceruloplasmin may be suggested to have positive influence regardless of copper with its antioxidant property whereas iron and cadmium have negative influence on sperm parameters and fertility in Merino rams. (C) 2015 Elsevier B.V. All rights reserved.Öğe Selected biochemical and oxidative stress parameters and ceruloplasmin as acute phase protein associated with bovine leukaemia virus infection in dairy cows(Natl Veterinary Research Inst, 2015) Akalin, Pinar Peker; Ataseven, Veysel Soydal; Dogan, Firat; Ergun, Yasar; Baspinar, Nuri; Ozcan, OguzhanThe aim of this study was to determine the ceruloplasmin (Cp) and vitamin C concentrations, the total antioxidant status (TAS), and selected biochemical parameters in dairy cows spontaneously infected with bovine leukaemia virus (BLV). Of the 27 cows included in the study, 18 animals were seropositive for enzootic bovine leukosis (EBL), whereas nine cows were seronegative and were used as controls. The serum aspartate aminotransferase (AST) (P = 0.003) and Cp concentrations (P = 0.03) decreased (65.17 +/- 5.03 and 7.70 +/- 0.72 respectively) in BLV-infected cows, as compared to healthy animals (100.67 +/- 11.50 and 10.40 +/- 0.70 respectively). A slight insignificant increase in alkaline phosphatase activity and unchanged levels of alanine aminotransferase, lactate dehydrogenase, calcium, magnesium, and TAS were demonstrated in EBL cows. As the TAS and vitamin C levels remained unchanged in EBL cows, it may be suggested that ruminants may compensate for the impaired oxidative/antioxidative balance. The results obtained also indicate that BLV may suppress AST and Cp synthesis or secretion in the liver through an unlcnown mechanism. The mechanism of action of BLV in hepatocytes, especially on AST and Cp, requires further investigation to elucidate the immune suppression caused by oncogenic retroviruses.Öğe Use of intestine-related biomarkers for detecting intestinal epithelial damage in neonatal calves with diarrhea(Amer Veterinary Medical Assoc, 2020) Ok, Mahmut; Yildiz, Ramazan; Hatipoglu, Fatih; Baspinar, Nuri; Ider, Merve; Uney, Kamil; Erturk, AlperOBJECTIVE To evaluate the usefulness of intestinal biomarkers in determining the presence of intestinal epithelial damage in neonatal calves with diarrhea caused by 4 etiologic agents. ANIMALS 40 neonatal calves that were healthy (n = 10) or had diarrhea (30). PROCEDURES The study was a cross-sectional study. Results of hematologic analyses and serum concentrations of intestinal fatty acid-binding protein (I-FABP), liver fatty acid-binding protein (L-FABP), trefoil factor 3 (TFF-3), Claudin-3 (CLDN-3), gamma-enteric smooth muscle actin (ACTG2), intestinal alkaline phosphatase (IAP), interleukin-8 (IL-8), platelet-activating factor (PAF), and leptin (LP) were compared among calves grouped according to whether they were healthy (control group; G-1) or had diarrhea caused by K99 Escherichia coli (G-2; n = 10), bovine rota-or coronavirus (G-3; 5 each), or Cryptosporidium spp (G-4; 10). RESULTS Across the 3 time points at which blood samples were obtained and evaluated, the groups of calves with diarrhea generally had markedly higher mean serum concentrations of L-FABP, TFF-3, IAP, IL-8, and LP, compared with the control group. In addition, G-2 also consistently had markedly higher mean serum concentrations of I-FAB and ACTG2 and lower mean serum concentrations of CLDN-3, compared with the control group. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that degree of intestinal epithelial damage differed among calves grouped by the etiologic agent of diarrhea and that such damage might have been more severe in calves with diarrhea caused by K99 E coli. Additionally, our results indicated that serum concentrations of I-FABP, L-FABP, TFF-3, IAP, IL-8, ACTG2, LP, and CLDN-3 were useful biomarkers of intestinal epithelial damage in calves of the present study.
