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    Effect of silibinin on the hyperlipidemia in rats fed with high cholesterol diet
    (Cukurova Univ, Fac Medicine, 2021) Duman, Didem; Arpaci, Abdullah; Dirican, Emre; Bozdogan, Server; Bayraktar, Hamdullah Suphi
    Purpose: Despite current hypolipidemic drugs, the search for a more effective hypolipidemic agent is ongoing. In this study, it was aimed to investigate the effect of Silibinin on hyperlipidemia in rats fed high cholesterol diet (HCD). Materials and Methods: Rats were made obese. Rats were given egg yolk for 60 days and then 50 mg/kg, 100 mg/kg Silibinin were applied i.p. for 7 days. Results: The first and last weights of the rats were significantly different. While total cholesterol (TC), LDL, TG and VLDL levels increased significantly in the groups fed with HCD, HDL level reduced compared to control group (CG). OxLDL and TAS were significantly different between groups. Conclusion: The effects of Silibinin on serum LDL, TC, VLDL, HDL, TG, OxLDL levels and to observe the antioxidant effect, TAS and TOS were investigated in experimental obese rat models. It was concluded that Silibinin plays an effective role in lowering TG and LDL levels, increasing HDL levels and decreasing hepatic lipid accumulation in HCD rats at 100 mg/kg dose. The use of Silibinin does not cause antihyperlipidemic effect but has antioxidant effect.
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    EFFECT OF SUBACROMIAL HYALURONIC ACID INJECTION ON THE NORMAL ROTATOR CUFF TENDON: AN EXPERIMENTAL STUDY IN RAT
    (2022) Duman, İbrahim Gökhan; Dogramacı, Yunus; Gökce, Hasan; Bayraktar, Hamdullah Suphi
    Ntroduction: Clinically hyaluronic acid injection has been used in treating shoulder pain and was found to be effective for patients with rotator cuff tears. The aim of the present experimental study was to evaluate cellular changes in the rotattor cuff after hyaluronic acid injection into subacromial area, compared to subacromial injections with normal saline. Material and Method: Ten male Wistar rats were used in the study. Both the right (study n=10) and left (control n =10) shoulders were used for subacromial injection in each rat. The hyaluronic acid doses were given 0.1ml of hyaluronic acid (HYALGAN®*) were injected subacromially into the right shoulder (s tudy group) and 0.1ml of saline solution were given into the subacromial space of the left shoulder (control group) once weekly for consecutive 3 weeks. One week after the last injection, the rats were sacrificed with a lethal dose of Xylazine HCl and Ketamine HCl. Next, the right and left shoulder joints including the supraspinatus and infraspinatus tendons were carefully dissected and slices were subjected to routine hematoxylin and eosin (H&E) staining procedure, and then were examined under a light microscope. Differences between groups, regarding animals’ weight, were evaluated using Student’s t-test for independent variables. The level of statistical significance was set at p<0.05. Results: Macroscopically: norma l tendon structures were observed in both groups. Microscopically: no statistically significant pathologic changes including necrosis, inflammation and giant cell infiltration were observed on microscopic examination of both the control and study shoulders (p= .5921). Conclusion: Hyaluronic acid injection into the subacromial area has no adverse histopathologic effects on the healthy rottator cuff.
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    Effects of Kefir on Blood Parameters and Intestinal Microflora in Rats: An Experimental Study
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2021) Ozsoy, Bulent; Cantekin, Zafer; Yalcin, Sakine; Bayraktar, Hamdullah Suphi
    A probiotic product of kefir is widely consumed by human beings. The purpose of this research was to investigate the effects of kefir on blood parameters and intestinal flora in rats. A total of 24 female rats were used in this study. During 35 days of experimental period, rats were fed with a commercial diet and water was provided ad libitum. Kefir was given at the levels of 10 mL/kg, 20 mL/kg and 30 mUkg with oral gavage to the first, second and third treatment groups, respectively. Kefir was not given to the control group. The number of yeast was found to be 1.65x10(8) and the number of lactobacilli was found to be 4x10(8) in kefir. At the end of the experiment, blood samples were taken from all rats. Blood plasma parameters and were investigated. The intestinal microflora was investigated by classical colony counting method. No differences were observed among the groups in total protein, albumin, uric acids, SGPT, SGOT, alkaline phosphatase and phosphorus in blood plasma. The plasma triglyceride and cholesterol levels in the second and third groups were lower than control group (P<0.05). No differences were observed in the intestinal pH levels among groups. Although total bacteria number of intestinal microflora was not different in groups, the number of Enterobactericeae and coliform bacteria in the third group was lower than the other groups (P<0.001). The number of Lactobacilli and the yeast level in the intestinal contents were increased by the usage of kefir (P<0.001). It was concluded that positive effects of the kefir were observed in intestinal microflora with increasing the number of beneficial bacteria and decreasing harmful bacteria and therefore kefir has a positive effect on the health of the animals.
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    Effects of Oleuropein Oral Intake on Infected Fat Grafts: Experimental Study
    (Springer, 2023) Bayraktar, Hamdullah Suphi; Okyay, Mehmet Fatih
    Aim The aim of this study was to evaluate the effects of oleuropein oral intake on infected fat grafts and fat graft survival.Materials and Methods 32 Wistar albino rats were divided into four groups: 0. none treated, 1. serum oral intake, 2. antibiotic oral intake, and 3. oleuropein oral intake. The dorsal regions of the rats were separated into four quadrants as right and left cranial, and right and left caudal to determine each quadrant where fat grafts were placed. Right cranial and caudal quadrants were infected with the methicillin-susceptible Staphylococcus aureus (MSSA) strain. The left cranial and caudal quadrants were infected with the Pseudomonas aeruginosa strain. On the 7th day and end of the 3rd month, fibroblast density, inflammation, and fat survival were demonstrated immunohistochemically with FGF, CD68, and perilipin (PP), respectively.Results On the 7th day, for P. aureginosa-infected grafts, oleuropein was shown higher rates in CD68 and PP staining compared to the antibiotic group (p < 0.05, p < 0.001, respectively). At the end of the 3rd month, for P. aureginosa and S. aureus-infected grafts, the oleuropein group was demonstrated improved PP staining rates compared to the antibiotic group (p < 0.01, p < 0.01, respectively).Conclusion Oleuropein as a natural olive leaf extract with potent antioxidant, anti-inflammatory, and antimicrobial features is an alternative and supportive agent for both treatment and prophylaxis of surgical site infections like the antibiotics of chemical synthesis. P. aeruginosa and S. aureus surgical site infections could treat and prevent safely and effectively by oleuropein, particularly in early and late periods after surgery.No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
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    Investigation of the effects of lipoic acid and dihydrolipoate on experimental renal ischemia-reperfusion model
    (2022) Kaçmaz, Filiz; Özcan, Oğuzhan; Arpacı, Abdullah; Ayaz, Ercan; Bayraktar, Hamdullah Suphi; Görür, Sadık
    Objective: Ischemic/reperfusion (I/R) causes tissue injury and the leading cause of acute kidney injury. In this study, we aimed to investigate the effects of the long and short-term usage of ALA and short-term DHLA on oxidative stress markers in the experimental renal ischemia-reperfusion model. Method: Forty male rats (250 to 300 gr) were divided into 5 groups: control; I/R group; long-term ALA+IR group; short-term ALA+IR group; and short-term DHLA+IR group. Ischemia was carried out for 45 minutes followed by reperfusion for 4 hours. Thiobarbituric acid reactive sunstances (TBARM), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities in tissue samples and serum total antioxidant status (TAS) and total oxidative stress (TOS) assayed by the spectrophotometrically. Tissue samples were investigated by histopathological analyzes. Results: TBARM (Control: 0.38±0.05. I/R: 1.37±0.17, long-term ALA-treated group:1.025±0.15, short-term ALA-treated group: 0.68±0.09, short-term DHLA- treated group: 0.38±0.04 (nmol/mg protein); p<0,001) CAT (Control: 0.12±0.02, I/R: 0.04±0.008, long-term ALA-treated group: 0.07±0.01, short-term ALA- treated group:0.06±0.008, short-term DHLA-treated group: 0.08±0.01 (k/mg protein); p<0.001), GSH-Px (Control: 0.45±0.04, I/R: 0.21±0.028, long-term ALA- treated group: 0.37±0.05, short-term ALA-treated group :0.34±0.05, short-term DHLA-treated group: 0.37±0.04 (U/mg protein); p<0.001), and serum OSI levels (Control: 1.32±0.15, I/R: 3.08±0.44, long-term ALA-treated group: 1.775±0.21, short-term ALA-treated group: 1.85±0.37, short-term DHLA-treated group: 1.53±0.21 (arbitrary unit) ; p<0.001) were improved in the long and short-term ALA-treated group and short-term DHLA-treated group compared to the I/R group. These findings were more prominent in histopathological tissue samples in the DHLA-treated group. Conclusion: We consider that both long-term and short-term ALA applications have the potential for the treatment of renal I/R damage. Besides, DHLA is more effective than ALA.
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    Klinik örneklerden izole edilen candida suşlarının PCR-RFLP yöntemiyle identifikasyonu
    (Hatay Mustafa Kemal Üniversitesi, 2013) Bayraktar, Hamdullah Suphi; Duran, Nizami
    ÖZET Klinik Örneklerden İzole Edilen Candida Suşlarının PCR-RFLP Yöntemiyle İdentifikasyonu Bu çalışmadaki amaç Candida türlerinin fenotipik dağılımlarını otomatize sistem kullanarak yapmak ve aynı zamanda Candida türlerinin polimeraz zincir reaksiyonu (PCR) ve Msp I ve Bln I enzimleri kullanılarak restriksiyon fragment uzunluğu polimorfizmi (RFLP) yöntemleriyle identifiye etmekti. Çeşitli klinik örneklerden izole edilen 150 Candida türü çalışmaya dahil edildi. Klinik izolatların fenotipik değerlendirmesi VITEK-2 otomatize identifikasyon sistemiyle yapıldı. Candida türlerinin genomik DNA ekstraksiyonları cam boncuklu kit kullanılarak yapıldı. Daha sonra amplifikasyon ürünleri Msp I ve Bln I restriksiyon enzimleriyle kesildi ve kesilen ürünler %3?lük agaroz jelde yürütüldü. Sonuçlar standart Candida suşları ile karşılaştırılarak değerlendirildi. Candida türlerinin büyük bir çoğunluğu idrar örneklerinden izole edildi (%78.6). Diğer klinik örneklerin oranları; vajinal sürüntü örnekleri %11.3 (17/150), yara örnekleri %3.3, kan örnekleri %2 ve diğer klinik örnekler %4.7 şeklindeydi. Fenotipik yöntemle yapılan identifikasyonda Candida türleri ve oranları; C. albicans %48.6, C. tropicalis %17.3, C. glabrata %17.3, C. parapsilosis %4.0, C. krusei %4.0, C. lusitaniae %0.6, C. kefyr %0.6, C. famata %0.6, C. lypolitica %0.6 ve diğer türler %6 olarak bulundu. Msp I ve Bln I restriksiyon enzimleri kullanılarak yapılan RFLP yöntemine göre ise oranlar; C. albicans %45.3, C. glabrata %19.3, C. tropicalis %.14.6, C. parapsilosis %5.3, C. krusei %5.3, C. lusitaniae %0.6 ve diğer türler %9.3 olarak belirlendi. Msp I ve Bln I restriksiyon enzimleri kullanılarak yapılan PCR-RFLP yöntemi C. albicans, C. glabrata, C.tropicalis, C. parapsilosis ve C. krusei türlerinin identifikasyonunda başarıyla kullanıldı. Candida türlerinin identifikasyonunda restriksiyon fragment ürünleri ilgili daha ileri çalışmalara ihtiyaç vardır. PCR-RFLP yöntemi, DNA sekans analizi gibi diğer sık kullanılan DNA bazlı yöntemlere göre ucuz ve daha az zaman alıcı bir yöntemdir. Bu avantajı sayesinde yöntemin kullanılabilirliği ve tanısal değeri artmaktadır.
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    Thymoquinone attenuates trauma induced spinal cord damage in an animal model
    (2014) Üstün, Nilgün; Aras, Mustafa; Özgür, Tümay; Bayraktar, Hamdullah Suphi; Sefil, Fatih; Özden, Raif; Yağız, Abdullah Erman
    BACKGROUND: Spinal cord injury (SCI) is one of the most devastating conditions leading to neurological impairment and disabilities. The aim of the study was to investigate the potential neuroprotective effect of thymoquinone (TQ) histopathologically in an experimental model of traumatic spinal cord injury (SCI). METHODS: Twenty-four male Wistar albino rats were randomly divided into 4 groups: control group; SCI group; SCI-induced and 10 mg/kg/day TQ administered group; SCI-induced and 30 mg/kg/day TQ administered group. TQ was given as intraperitoneal for three days prior to injury and four days following injury. Spinal cord segment between T8 and T10 were taken for histopathologic examination. Hemorrhage, spongiosis and liquefactive necrosis were analyzed semiquantatively for histopathological changes. RESULTS: Administration of TQ at a dose of 10 mg/kg did not cause any significant change on the histological features of neuronal degeneration as compared to the SCI group (p=0.269); however, 30 mg/kg TQ significantly decreased the histological features of spinal cord damage below that of the SCI group (p=0.011). CONCLUSION: Data from this study suggest that TQ supplementation attenuates trauma induced spinal cord damage. Thus, TQ needs to be taken into consideration, for it may have a neuroprotective effect in trauma induced spinal cord damage