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Öğe ANTIMICROBIAL EFFICACY OF Origanum syriacum L. AND Origanum onites L. ESSENTIAL OILS AGAINST Pseudomonas aeruginosa AND THEIR EFFECTS ON BIOFILM FORMATION(Incdtp-Icpi, 2018) Bayraktar, Suphi; Duran, NizamiIn this study, we aimed to search the components and antimicrobial activity of Origanum syriacum and Origanum onites essential oils against Pseudomonas aeruginosa and also investigated the effects of these two plants essential oils on biofilm formation of Pseudomonas aeruginosa. Seventeen essential oil components, representing 98,76 %, were detected in the essential oil of Origanum syriacum L. and the major essential oil constituents were thymol (42,18 %), carvacrol (33,95 %), cymene (8,87 %) and gamma-terpinene (8,21%). Thirty-four essential oil components, representing 98,62 %, were detected in the essential oil of Origanum onites L. with major essential oil constituents were thymol (68,28 %), gamma-terpinene (5,50 %), p-cymene (5,47 %) and linalool (4,40%). In the study, Origanum onites essential oils were found to be more effective against both amikacin-resistant Pseudomonas aeruginosa strains and amikacin susceptible strains. In addition, the combination of Origanum syriacum and Origanum onites essential oils showed stronger efficacy against both amikacin-resistant and susceptible strains of Pseudomonas aeruginosa. The combined use of Origanum syriacum and Origanum onites essential oils showed stronger biofilm inhibition activity in all Pseudomonas strains. In the present study, the remarkable efficacy of Origanum syriacum and Origanum onites essential oils against both drug-resistant and drug-sensitive Pseudomonas aeruginosa strains was determined. More importantly, it has been found that the ability of these bacteria to form biofilms was inhibited by increasing concentrations of essential oils of these plants.Öğe Effect of alpha-lipoic acid on endometrial implants in an experimental rat model(Wiley, 2017) Pinar, Neslihan; Karapinar, Oya Soylu; OEzcan, Oguzhan; Ozgur, Tumay; Bayraktar, SuphiTo investigate the antioxidant and anti-inflammatory effects of alpha-lipoic acid (ALA) in the treatment of endometriosis in an experimental rat model by evaluating biochemical and histopathologic parameters. Experimental endometriosis was induced by the peritoneal implantation of autologous endometrial tissue. The rats were randomly divided into two groups with eight rats each. Group I was intraperitoneally administered ALA 100 mg/kg/day for 14 days. Group II was intraperitoneally administered saline solution at the same dosage and over the same period. Endometrial implant volume was measured in both groups both pre- and post-treatment. Tumor necrosis factor alpha (TNF-) was measured in peritoneal fluid. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were assessed in serum. The implants were histopathologically evaluated. In the ALA group, the serum TOS and OSI levels, the endometrial implant volumes, the TNF- levels in serum and peritoneal fluid, and the histopathologic scores were significantly lower compared to the control group (P < 0.05). Alpha-lipoic acid may have a therapeutic potential in the treatment of endometriosis due to its antioxidant and anti-inflammatory effects.Öğe The effect of dexpanthenol on experimentally induced ovarian ischaemia/reperfusion injury: a biochemical and histopathological evaluation(Springer Heidelberg, 2017) Karapinar, Oya Soylu; Pinar, Neslihan; Ozcan, Oguzhan; Dogan, Esin Atik; Bayraktar, Suphi; Sahin, Hanifi; Dolapcioglu, KenanThe aim of this study was to evaluate the effect of two different doses of dexpanthenol (Dxp) onexperimentally induced ovarian ischaemia/reperfusion (I/R) injury ina rat model. Forty female rats were randomly divided into fivegroups: Group 1: sham operation; Group 2: 3-h ischaemia; Groups 3: 3-h ischaemia, 3-h reperfusion (I/R); Group 4: I/R + 300 mg/kg Dxp intraperitoneally (i.p) Group 5: I /R + 500 mg/kg Dxpi.p. Total anti-oxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), tissue malondialdehyde (MDA) and activities of glutathione peroxidase and catalase were calculated. Ovarian tissue damage was assessed using a histopathological scoring system. The TOS and OSI values were significantly lower in Group 5, as compared to Groups 2 and 3 (p < 0.05). The MDA levels in Group 1 and Group 5 were significantly lower than those in Group 3 (p < 0.05). CAT and GSH-Px activity was higher in Group 5 than in Group 2 and Group 3 (p = 0.00). Tissue damage scores were elevated in all the groups compared with sham group, but the treatment with the different doses of Dxp before reperfusion ameliorated the tissue damage scores. The results showed that Dxp reduced ovarian I/R injury.Öğe Effects of caffeic acid phenethyl ester on renal damage in rabbits with partial ureteral obstruction(2009) Akçora, Bülent; Altu?, Muhammed Enes; Hakverdi, Sibel; Kontaş, Tünay; Öztürk, Atakan; Bayraktar, SuphiObstructive urologic diseases may cause renal injury related to intensty and duration of occlusion. In experimental studies, many pharmacological agents were used to decrease the harmful effects of obstruction on kidney. This study was designed to investigate effects of caffeic acid phenethyl ester (CAPE) on the parsial ureteral obstruction in rabbits. Twenty-one New Zealand rabbits were divided randomly into 3 groups each containing 7 animals. Group 1, 2 and 3 were defined as control (sham), partially obstructed, and partially obstructed plus CAPE treatment, respectively. All animals were sacrified at the end of 3 weeks. Malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and myeloperoxidase (MPO) levels were measured. In addition, kidney tissues stained with Hematoxylin-eosine were evaluated using Cleasson's histopathological criteria. MDA, NO and SOD levels were not significantly different among all groups. Mean MPO levels of groups 1, 2 and 3 were 0.22±0.07 U/g protein, 0.36±0.09 U/g protein, 0.27±0.10 U/g protein respectively. A significant increase was found in group 2 when compared to group 1(p<0.05). Kidneys of group 3 were significantly protected in terms of 2 out of 9 histopathologic criteria when compared to group 2 (p<0.05). There was significant difference between group 2 and group 3 in terms 2 out of 9 histopathologic criteria (p<0.05); injury of kidneys in group 3 was less than in group 2. MDA, NO and SOD levels which are used to detect renal injury in complete ureteral obstruction model, did not show any significant difference in partial obstruction model in our study. In the light of our biochemical and histopathologic findings, we can say that CAPE treatment has a tendency to decrease the degree of renal injury in the partial obtruction model. © 2009 OMÜ Tüm Haklari Saklidir.Öğe Effects of Erdosteine and Vitamin D in Experimental Rat Kidney Ischemia/Reperfusion Model(Wiley, 2017) Dogan, Hatice; Demir, Enver Ahmet; Tutuk, Okan; Ozgur, Tumay; Kucuk, Meral Urhan; Ozcan, Oguzhan; Bayraktar, Suphi[Abstract Not Available]Öğe Effects of Lipoic Acid on Testicular Cell Damage Induced by Experimental Varicocele Model(Wiley, 2017) Tutuk, Sumeyye; Ozcan, Oguzhan; Tutuk, Okan; Ozgur, Tumay; Dogan, Hatice; Bayraktar, Suphi; Tumer, Cemil[Abstract Not Available]Öğe Effects of nigella sativa l.’s essential oils on multi drug resistant escherichia coli isolates(Bulgarian Society for Microbiology (Union of Scientists in Bulgaria), 2018) Duran, Nizami; Ay, Emrah; Bayraktar, Suphi; Çolak, Sezin; Kaya, Durmuş AlpaslanEscherichia coli is one of the most important human pathogens and is a major cause of nosocomial infections. In this study, it was aimed to investigate the antimicrobial efficacy of Nigella sativa L. against multi drug-resistant E. coli isolates. This study was carried out on multi drug resistant E. coli isolates. In this study, seeds of Nigella sativa L. obtained from spice market were used as material.Multidrug resistant E. coli strains were identified by conventional methods. The antibacterial activity of Nigella sativa L.’s essential oils was performed by disk diffusion test. In addition, when needed, automated systems were used for rapid identification of microorganisms and susceptibility testing identification. The ESBL production of these strains was confirmed by double disc synergy test.In ESBL producing E.coli strains, the MIC range of ampicillin was 4-32 ?g/ml and the MIC range of cefuroxime was found to be 2-16 ?g/ ml. MIC values of ampicillin were range from 0.25 to 4 ?g/ml and MIC of levofloxacin range were 1 to 8 ?g/ml and MIC of cefuroxime range were found to be 0.25-4 ?g/ml. Following treatment with essential oils of Nigella sativa, resistance rates of GSBL producing E.coli strains against all these three antibiotics (ampicillin, levofloxacin and cefuroxime) have been found to decrease statistically significantly (p<0.05). Nigella sativa L.’s essential oils showed complete zone of inhibition of the standart E.coli strains (p<0.05). Besides this, in experiments with multi drug resistant E.coli isolates, it was found that the inhibition zones of Nigella-treated GSBL producing E.coli strains differed from non-treated strains. There was a statistically significant increasing in inhibition zones in drug E.coli isolates (p<0.01).The essential oils of Nigella sativa L. may contain promising antimicrobial components in the treatment of multi drug-resistant Ecoli isolates. This is very important for the discovery of new antimicrobial agents. More extensive clinical trials should be undertaken in this regard. © 2018, Bulgarian Society for Microbiology (Union of Scientists in Bulgaria). All rights reserved.Öğe The frequency of shiga-like toxin (stx1 and stx2) and EHEC-hlyA in food by multiplex PCR(Univ Press, 2017) Onlen, Cansu; Duran, Nizami; Bayraktar, Suphi; Ay, Emrah; Ozer, BurcinAim: The aim of the present study was to determine the frequency of shiga-like toxin (stx1 and stx2) and drug resistance profiles food-borne Escherichia coli O157: H7 in Hatay province, Turkey. Methods: The presence of the virulence genes (stx1, stx2, hlyA) in a total of 150 E. coli isolates were studied with multiplex PCR. Results: A total of 327 salad samples were analyzed. E. coli O157: H7 was detected in 150 (45.8 %) out of 327 analyzed samples. Of these 150 isolates, the presence of hly-A gene was detected in 32 (21.3%) E. coli isolates. A total of five (15.6%) isolates in this 32 hlyA positive isolates had stx2 gene, two (6.3%) of them had stx1 gene and one (3.1%) of the isolates was found to be positive for both stx1 and stx2 genes. It was found that all E. coli O157: H7 isolates were resistant to erythromycin. While the highest rate of antibiotic resistance was observed for ampicillin (68.8%), no antibiotic resistance against cefuroxime, ciprofloxacin and cephaperasone was identified. Conclusions: The results obtained in our province showed that E. coli strains isolated from salad samples were found to have some important virulence genes such as stx1, stx2, and hlyA. The stx2 frequency was found to be higher than stx1 frequency. Also, it was observed that there was not any significant correlation between drug resistance profiles and presence of toxin genes in E. coli O157: H7 strains. As a result, increasing frequency of STEC O157 serotype among foodborne pathogens is a growing public health problem.Öğe Identification of Medically Important Candida Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of the rDNA ITS1 and ITS2 Regions(Elsevier, 2017) Bayraktar, Suphi; Duran, Nizami; Duran, Gulay Gulbol; Eryilmaz, Naciye; Aslan, Hayat; Onlen, Cansu; Ozer, BurcinAim: We aimed to identify the distribution of species in candidal strains isolated from clinical samples and restriction fragment length polymorphism (RFLP) method based on Msp I and Bln I restrictive enzyme cuts of polymerase chain reaction (PCR) products after the amplification of ITS1 and ITS2 regions of rDNA genotypically. Materials and Methods: One hundred and fifty candidal strains isolated from various clinical samples were studies/ included. Phenotypic species assessment was performed using automated VITEK-2 system and kit used with the biochemical tests. Common genomic region amplification peculiar to candidal strains was carried out using ITS1 and ITS2 primer pairs. After the amplification, PCR products were cut with Msp I and Bln I restriction enzymes for species identification. Results: The majority of Candida isolates were isolated from urine (78.6%) while other isolates were composed of strains isolated from swab, wound, blood and other samples by 11.3%, 3.3%, 2% and 4.7%, respectively. The result of RFLP analysis carried out with Msp I and Bln I restriction enzymes showed that candidal strains were Candida albicans by 45.3%, Candida glabrata by 19.3%, Candida tropicalis by 14.6%, Candida parapsilosis by 5.3%, Candida krusei by 5.3%, Candida lusitaniae by 0.6% and other candidal strains by 9.3%. Conclusion: When the ability to identify Candida to species level of phenotypic and PCR-RFLP methods was assessed, a great difference was found between these two methods. It may be argued that Msp I and Bln I restriction enzyme fragments can be used in the identification of medically important Candida species. Further studies are needed to develop this kind of restriction profile to be used in the identification of candidal strains.Öğe Oxidative Status and Varicocele: The Effect of Lipoate(Wiley, 2017) Demir, Enver Ahmet; Tumer, Cemil; Tutuk, Okan; Dogan, Hatice; Tutuk, Sumeyye; Bayraktar, Suphi[Abstract Not Available]Öğe Protective effects of tempol in an experimental ovarian ischemia-reperfusion injury model in female Wistar albino rats(Canadian Science Publishing, 2017) Pinar, Neslihan; Karapinar, Oya Soylu; Ozcan, Oguzhan; Dogan, Esin Atik; Bayraktar, SuphiThe aim of this study was to investigate the antioxidant effects of tempol on ovarian ischemia-reperfusion (I/R) injury in rats. Forty female Wistar albino rats were randomly divided into 5 groups: Group I, sham; Group II, ischemia (I); Group III, I/R; Group IV, I/R + tempol 30 mg/kg i.p; Group V, I/R + tempol 50 mg/kg i.p. Oxidative stress index (OSI) was significantly higher in the ischemia group and the I/R group than in the sham group. Catalase levels were significantly lower in the I/R group than in the I/R + tempol 30 mg/kg i.p. and the I/R + tempol 50 mg/kg i.p. groups. Glutathione peroxidase levels were lower in the I/R group than in the I/R + tempol 30 mg/kg i.p. and the I/R + tempol 50 mg/kg i.p. groups. MDA levels were significantly lower in the I/R + tempol 30 mg/kg i.p. group and the I/R + tempol 50 mg/kg i.p. group than in the I/R group. The levels of the histopathological parameters were significantly decreased in the I/R + tempol 50 mg/kg i.p. group compared with the I/R group. Tempol can be used for reducing ovarian I/R injury.Öğe The therapeutic potential and molecular mechanism of Alpha-pinene, Gamma-terpinene, and P-cymene against melanoma cells(Cell Press, 2024) Acikgul, Funda Cimen; Duran, Nizami; Kutlu, Tuncer; Ay, Emrah; Tek, Erhan; Bayraktar, SuphiThe purpose of this study is to investigate the potential anticarcinogenic effects of three phytochemicals, namely Alpha-pinene (AP), Gamma-terpinene (GT), and P-cymene (PC), on melanoma cells (A2058 cell line). Additionally, the study aims to explore the synergistic activities of these phytochemicals with Dacarbazine, a chemotherapy drug. To understand the molecular mechanism involved in apoptosis induction in the A-2058 cell line, it was used AO/EB staining for apoptosis detection and cell cycle analysis, monitored through flow cytometry. It also determined the mRNA expression levels of different apoptosis-regulatory genes, including p53, Bax, NF-kB, Bcl-2, Bcl-xl, and caspase-3. The antitumor activities of these phytochemicals and their combinations were investigated in a subcutaneous mouse tumor model. The tumor diameter was 21.4 f 1.1 mm in the Dacarbazine treatment group and 42.4 f 3.1 mm in the control group. The antitumoral activities of AP and PC in the tumor model were similar to those of Dacarbazine. On the other hand, GT exhibited remarkable antitumoral activity, with a 1.75-fold reduction in tumor diameter compared to the Dacarbazine group. When different combinations of phytochemicals and Dacarbazine were used, the GT plus Dacarbazine treatment group was found to have a 3.5- fold reduction in tumor diameter compared to the Dacarbazine group. The tumor diameters in the Dacarbazine, AP plus GT, GT plus Dacarbazine, and AP plus Dacarbazine treatment groups were 21.4 f 1.1, 7.6 f 2.2, 8.6 f 0.5, and 6.2 f 1.9 mm, respectively.
