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  • Küçük Resim
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    Effects of Interactions among Gene Polymorphisms of the Renin-Angiotensin-Aldosterone System on Hypertension in Turkish People from Southeast Anatolia
    (Sciendo, 2019) Arpaci, Abdullah; Urhan-Kucuk, Meral; Bayramoglu, Aysegul; Guler, Halil Ibrahim; Ecevit, Hasret; Suner, Arif; Karakas-Celik, Sevim
    Introduction: Hypertension (HT) is characterized by high blood pressure. The reninangiotensin-aldosterone system (RAAS) plays a crucial role in blood pressure (BP) regulation by maintaining vascular tone and the water-sodium balance. We aimed to investigate whether there is any relation between AGT (M235T), ACE (I/D), and AGTR1 (A1166C) genetic polymorphisms and hypertension among Turkish people from Southeast Anatolia. Method and Results: A total of 210 individuals, consisting of 102 healthy controls and 108 patients with essential hypertension admitted to the Cardiology Department of the Adiyaman University Research and Application Hospital were included the study. DNA isolation was performed from the blood samples via commercial kit. Genotype determination was determined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Statistically significant differences were found between the control and patient groups in terms of genotype distribution and allelic frequencies of ACE I/D polymorphisms. Significant differences were found in the frequencies of ICM, DCM, DCT, DAT between the patient and control groups. Conclusions: In this study, we found a significant association of ACE I/D polymorphism with HT, and we showed that the I allele can increase the risk of HT in Turkish people from Southeast Anatolia. Although we did not find any association between independent AGT M235T and AGT1R A1166C polymorphisms and HT, we observed that the DCM, DCT, and DAT haplotypes of ACE/AGT1R/AGT polymorphisms reduced the risk of hypertension, while the ICM haplotype increased it.
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    Öğe
    Genetic variations of renin-angiotensin and fibrinolytic systems and susceptibility to coronary artery disease: a population genetics perspective
    (Edizioni Minerva Medica, 2022) Bayramoglu, Aysegul; Bayramoglu, Gokhan; Urhan Kucuk, Meral; Guler, Halil, I; Arpaci, Abdullah
    BACKGROUND: Genetic predisposition is an important risk factor in coronary artery disease (CAD).This study was conducted to determine the polymorphism frequencies of the plasminogen activator inhibitor-1(PAI-1) gene 4G/5G, angiotensin-converting enzyme (ACE) gene I/D, and angiotensin II type 1 receptor (AT1) gene A1166C genotypes and to examine the role of these polymorphisms in CAD. METHODS: Genomic DNAs obtained from 260 subjects (130 CAD patients and 130 control) were used in the study. ACE I/D and PAI-1 4G/5G polymorphism genotypes were determined using polymerase chain reaction (PCR) and electrophoresis. AT-1 A1166C polymorphism was determined using the PCR, restriction fragment length polymorphism (RFLP) and electrophoresis. The products amplified from AT1 gene by PCR were cut with HindIII restriction endonuclease and then analyzed by 2% agarose gel electrophoresis. The results were statistically analyzed with the chi-square test, Mann-Whitney U test, and independent two-sample t-test. RESULTS: Allele frequencies showed statistically significant differences between the patient and control groups. There was no statistically significant difference in ACEI/D genotype frequencies between the twogroups. Likewise, no statistically significant difference was found in the AT1 A1166C genotype frequencies; however, a statistically significant difference was found in allele frequencies. The PAI-1 4G/5G genotype frequency was significantly higher in the patient group. CONCLUSIONS: While there is a relationship between of PAI-1 gene 4G/5G polymorphism and CAD, ACE gene I/D and AT1 gene A1166C polymorphisms are not related. PAI-1 gene homozygous genotypes may be considered as a prognostic marker for CAD patients.
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    Investigation of relationship between IL-6 gene variants and hypertension in Turkish population
    (Springer, 2015) Karaman, Esin; Kucuk, Meral Urhan; Bayramoglu, Aysegul; Gocmen, Semire Uzun; Ercan, Suleyman; Guler, Halil Ibrahim; Kucukkaya, Yunus
    Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a polymorphic gene which -174 G/C is a common and -572 G/C is a rare polymorphisms identified in promoter region. Publications on IL-6 gene polymorphisms raised the question whether this gene polymorphisms lead to susceptibility to HT or not. To investigate the effects of IL-6 gene -174 G/C (rs 1800795) and -572 G/C (rs1800796) polymorphisms on plasma IL-6 and CRP levels and their associations with hypertension disease in Turkish population we analyzed -174 G/C and -572 G/C polymorphisms and plasma IL-6 and CRP levels in 111 healthy controls and 108 hypertension patients from Adyaman, Turkey. We determined the genotypes using polymerase chain reaction-restriction fragment length polymorphism and analyzed plasma levels of IL-6 by ELISA and CRP by automated standard biochemical methods. We have found no statistically significant differences between IL-6 gene -174 G/C and -572 G/C genotypes and allelic frequencies and IL-6 and CRP plasma levels and HT (p > 0.05). No CC genotype was found in control subjects for -572 G/C polymorphism. In conclusion, we found relation to -174 G/C and -572 G/C gene variants between neither IL-6 and CRP levels nor hypertension. The -572 G allele and GG genotype are predominant in Turkish population in Adyaman, Turkey whereas the CC genotype is very rare.
  • Küçük Resim
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    Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hypertension?
    (Springer, 2015) Bayramoglu, Aysegul; Urhan Kucuk, Meral; Guler, Halil Ibrahim; Abaci, Okay; Kucukkaya, Yunus; Colak, Ertugrul
    The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hypertension and 99 healthy controls) were used in the study. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism and electrophoresis. The results were statistically analyzed and were found to be statistically significant. The frequencies of the C1562T genotypes were found to be, in controls CC 75.8 % and CT 24.2 % and in patients CC 71.2 %, and CT 28.8 %. The frequencies of C677T genotype were found to be, in controls CC 56.6 %, CT 38.4 and TT 5.1 % in controls and in patients CC 52 %, CT 30.4 % and TT 17.6 %. In conclusion, we may suggest that there is no relation between the essential hypertension and C1562T polymorphism of MMP-9 gene; on the other hand C677T polymorphism (genotype TT) of MTHFR gene can be regarded as a genetic indicator for the development of essential hypertension.