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    Detection and Sequence Analysis of Equine Gammaherpesviruses from Horses with Respiratory Tract Disease in Turkey
    (Wiley, 2010) Ataseven, V. S.; Bilge-Dagalp, S.; Oguzoglu, T. C.; Karapinar, Z.; Guzel, M.; Tan, M. T.
    P>The equid herpesvirus 2 (EHV-2) and 5 (EHV-5), identified agents of respiratory infections and keratoconjunctivitis cases in some equids, comprise a high degree of antigenic heterogeneity. Prevalence and genetic characterization of EHV-2 and EHV-5 strains from Turkey were investigated in this study. A total of 73 nasal swabs and 54 blood specimens were sampled from horses with respiratory tract diseases characterized by mucopurulent nasal discharge and occasional coughing. Overall, EHV-2- and EHV-5-specific DNA amplicons were obtained from 19.2% (14/73) and 21.9% (16/73) of horses tested by multiplex nested PCR. Sequences of EHV-2 and EHV-5 glycoprotein B (gB) gene were used in a phylogenetic analysis that included six EHV-2 and three EHV-5 isolates, which showed that the Turkish EHV-2 and EHV-5 strains have marked sequence divergence from European strains and from each other. Turkish EHV-2 isolates were divided into two distinct subdivisions, and a few isolates were located on a separate branch. This study provides the first epidemiological and phylogenetical report about EHV-2 and EHV-5 infections in Turkey.
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    Öğe
    Partial sequence of the ORF1b gene fragment of equine arteritis viruses detected in Turkey and phylogenic analysis
    (Ecole Nationale Veterinaire Toulouse, 2013) Ataseven, V. S.; Oguzoglu, T. C.; Karapinar, Z.; Bilge-Dagalp, S.
    The aim of this study was to document the first genetic information based on partial sequences of the ORF1b gene of nine Turkish (TR) equine arteritis virus (EAV) strains obtained from horses showing signs of respiratory tract disease. Partial sequencing of the PCR products based on ORF1b gene using EAV specific primer pairs followed amplification and purification was performed and was compared to European and American viruses recoded in GenBank to phylogenic analysis. The TR virus strains were found closely related to the strains that originated in North America and exhibited marked sequence heterogeneity from European strains and additionally the TR strains were relatively homogeneous and were placed in a separate cluster. This study documents for the first time the genetic of EAV genotypes in Turkey and will provide valuable insight for future studies on the molecular epidemiology of EAV.