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Öğe Chemical Composition of Myrtus communis L. and Proapoptotic Effects on the A549 Cell Line(Taylor & Francis Ltd, 2020) Bilgic, Nilufer; Duran, Gulay GulbolThe aim of this study was to analyze the essential oils of Myrtus communis L.from the south of Turkey by the GC/MS method and to demonstrate its proapoptotic effects on A549 cells. A549 cells were used as cell lines in the study. Cells were treated for 24 hours with a different concentration of the essential oil (from 200 to 31.25 mu g/ml). Non-toxic concentrations of essential oils of MC were determined by the MTT method. Apoptosis studies were performed within the determined non-toxic concentrations of essential oils. Expression levels of the caspase 3, caspase 8, caspase 9, Bcl-2, and p21 genes were performed by qRT-PCR method. Eucalyptol (1,8-cineole; 31.2 %) was found at the highest rate in the essential oils content, while alphapinene followed it with a rate of 29.6 %. Our results showed that essential oil concentrations of 625 mu g/ml and higher than this concentration significantly suppressed cell viability at the end of the 24-hour incubation compared to the control group. The expression levels of caspase 3 and caspase 9 genes, which are the mediators of the intrinsic pathway, increased statistically significantly at the essential oil concentration of 312.5 mu g/ml (respectively, p=0.0129 and p=0.0180). The expression level of the p21 gene was significantly increased at a concentration of 156.25 mu g/ml essential oils (p=0.0002). The essential oils of MC from the South of Turkey have been found to induce apoptosis intrinsically. These findings could be a guide in understanding the molecular mechanism underlying the anti-cancer properties of MC's essential oils on A549 cells. We think that it has the potential to be a new natural therapeutic agent for cancer treatment.Öğe Detrimental effects of chia (Salvia hispanica L.) seeds on learning and memory in aluminum chloride-induced experimental Alzheimer's disease(Nencki Inst Experimental Biology, 2018) Bilgic, Yasemin; Demir, Enver Ahmet; Bilgic, Nilufer; Dogan, Hatice; Tutuk, Okan; Turner, CemilPolyphenols and omega-3 fatty acids are thought to have beneficial effects in Alzheimer's disease, the most common cause of dementia. Seeds of chia (Salvia hispanica L.) are highly rich in these nutrients, and thus, the present study investigated the effects of chia seeds on behavior and cognition in an aluminum-induced Alzheimer's disease model in rats. Experimental animals received chia supplementation either during the generation of the model (i.e., pretreatment) or after the model was established (i.e., treatment). A battery of behavioral and cognitive tests were performed, including open-field, elevated plus maze, Porsolt's forced swim, and Morris' water maze, to evaluate anxiety- and depression-like behaviors, and learning and memory. Results showed that chia supplementation was ineffective against Alzheimer's-related anxiety, whereas depression-like behaviors were attenuated with both pretreatment and treatment. There was no improvement in learning and memory with chia treatment. Rather, cognitive performance in chia-pretreated animals was remarkably worse as compared to their non-treated disease-induced counterparts. Hippocampal concentrations of amyloid-beta 42, amyloid precursor protein, and total tau protein were similarly increased in all disease-induced animals (despite chia supplementation), as compared to the controls. Based on these findings, chia supplementation during the progression of Alzheimer's disease may exacerbate the disease. Although the results presented here emerge from an experimental/preclinical study, we suggest cautious and careful use of chia, especially in early-stage Alzheimer's patients, until future research in different experimental settings is conducted.Öğe The Effect of Chia (Salvia hispanica L.) Seed in an Experimental Alzheimer's Disease Model(Wiley, 2018) Bilgic, Yasemin; Demir, Enver Ahmet; Bilgic, Nilufer; Dogan, Hatice; Tutuk, Okan; Tumer, Cemil[Abstract Not Available]Öğe Effects of Alpha-Lipoic Acid on Urotensin II and TGF-?1 on Steroid-induced Osteonecrosis in Rats(Wiley-Blackwell, 2016) Dokuyucu, Recep; Tutuk, Okan; Dogan, Hatice; Bilgic, Nilufer; Tas, Zeynel Abidin; Sefil, Fatih; Gogebakan, Bulent[Abstract Not Available]Öğe Investigation of the Effect of CAPE on Urotensin II and TGF-?1 Levels on Experimental Osteonecrosis Rat Model(Wiley-Blackwell, 2016) Dokuyucu, Recep; Tutuk, Okan; Gogebakan, Bulent; Dogan, Hatice; Bilgic, Nilufer; Tas, Zeynel Abidin; Sefil, Fatih[Abstract Not Available]Öğe Investigation of the Effect of Verapamil on Hepatic Ischemia Reperfusion Injury(Wiley-Blackwell, 2016) Dokuyucu, Recep; Dogan, Hatice; Izmirli, Muzeyyen; Bilgic, Nilufer; Gokce, Hasan[Abstract Not Available]Öğe The Role of Lidocaine on Transient Receptor Potential Melastatin (TRPM) 6,7 Channels in Hepatic Ischemia/Reperfusion Rat Model(Wiley-Blackwell, 2016) Dokuyucu, Recep; Tutuk, Okan; Dogan, Hatice; Izmirli, Muzeyyen; Bilgic, Nilufer; Gokce, Hasan[Abstract Not Available]
