Yazar "Borazan, A." seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim
    Öğe
    THE EFFECTS OF DARBEPOETIN ON PERITONEAL FIBROSIS INDUCED BY CHEMICAL PERITONITIS AND ON PERITONEAL TISSUE MMP-2 AND TIMP-2 LEVELS IN RATS
    (Sage Publications Inc, 2009) Borazan, A.; Camsari, T.; Cavdar, Z.; Sarioglu, S.; Yilmaz, O.; Oktay, G.; Sifil, A.
    The aim of this study is to assess the influence of darbepoetin on the development of peritoneal fibrosis in rats induced by chlorhexidine gluconate (0.1%) and ethanol (15%) and to determine the effect on peritoneal tissue levels of MMP-2 and TIMP-2, possible important factors in progression of peritoneal fibrosis. Twenty-four female Wistar albino rats were divided into three groups. The first group (CH group) received 3 ml/200g daily intraperitoneal injections of chlorhexidine gluconate (0.1%) and ethanol (15%) dissolved in saline to induce chemical peritonitis; group 2 (ESA group) received 3 ml/200g daily injections of chlorhexidine gluconate (0.1%) and ethanol (15%) dissolved in saline and also darbepoetin 12.5 microgr/per kilogram/day subcutaneously on the first and seventh days; group 3 (Control group) received intraperitoneal 0.9% saline (3 ml/200g/d) through the right lower quadrant by 21 gauge needle. The study duration was fourteen days. On the fifteenth day rats were sacrificed, parietal peritoneum samples were obtained from the left anterior abdominal wall. Pathological samples were examined using Hematoxyline & Eosin (HE) stains. The thickness, vasculpathy, and inflammation were determined by light microscopy. MMP-2 and TIMP-2 were studied immunohistochemically by monoclonal antibody staining. The activity of MMP-2 on peritoneal tissue was studied by gelatin zymography and TIMP-2 protein level was analysed by ELISA, biochemically. The decrease in thickness of parietal peritoneum in group ESA was statistically significant when compared to CH group (p<0.05). Inflammation scores, and vascularization score surfaces were not statistically different between these groups (p>0.05). Immunohistochemically, darbepoetin was shown to decrease MMP-2 expression on parietal peritoneum in CH group (p<0.05), but had no effect on TIMP-2 (p>0.05). Biochemically the ratio of active MMP-2 to proMMP-2 was more significantly increased in the ESA group than in the CH group (p<0.001), however, TIMP-2 levels in both groups were decreased compared to the control group (p<0.05). Darbepoetin histopathologically reduced peritoneal fibrosis induced by chlorhexidine gluconate. We can suggest that Darbepoetin does not cause peritoneal fibrosis and may prevent peritoneal fibrosis in rats possibly related to an effect on MMP-2 expression. Further research regarding the utility and dosage should be considered.
  • Küçük Resim
    Öğe
    The Effects of Interferon ?2b on Chemically-induced Peritoneal Fibrosis and on Peritoneal Tissue MMP-2 and TIMP-2 Levels in Rats
    (Field House Publishing Llp, 2010) Ucar, E.; Borazan, A.; Semerci, E.; Binici, D. N.; Yaldiz, M.; Aysal, A.; Altug, E.
    This study investigated the effect of interferon alpha 2b on chlorhexidine gluconate (CH)-induced peritoneal fibrosis (PF) in rats and assessed peritoneal tissue levels of metalloproteinase (MMP)-2 and tissue inhibitors of metalloproteinases (TIMP)-2. Wistar albino rats (n = 8 per group) were treated as follows: control group, 3 ml/day of 0.9% saline intraperitoneally for 28 days; CH group, 0.1% CH (200 g [3 ml]/day) in 15% ethanol and 0.9% saline intra-peritoneally for 28 days; CH + interferon (IFN) group, CH (as above) plus pegylated IFN-alpha 2b 1.5 mu g/kg per week subcutaneously on days 0, 7, 14, 21 and 28; IFN group, pegylated IFN-alpha 2b (as above). Parietal peritoneum samples were obtained from the left anterior abdominal wall after 35 days. Parietal thickness, degree of vascular proliferation and inflammation, and MMP-2 and TIMP-2 levels were determined. The mean peritoneal thicknesses of the control, CH, CH + IFN and IFN groups were 7.02 +/- 3.89, 156.86 +/- 29.13, 59.88 +/- 22.1, 9.27 +/- 2.03 mu m, respectively. Pegylated IFN-alpha 2b decreased CH-induced expression of MMP-2 in the parietal peritoneum, but had no effect on TIMP-2 levels. Further studies are needed to determine the optimal dosage and duration for pegylated IFN-alpha 2b treatment.
  • Küçük Resim
    Öğe
    RELATIONSHIP BETWEEN METABOLIC SYNDROME AND INFLAMMATION IN PERITONEAL DIALYSIS PATIENTS
    (Sage Publications Inc, 2012) Borazan, A.; Ucar, E.; Gursu, M.; Emir, I.; Ozturk, Y.
    We aimed to investigate the relationship between inflammation and metabolic syndrome as defined by the National Cholesterol Education Program Adults Treatment Panel III (NCEP-ATP III) diagnostic criteria in peritoneal dialysis patients. Ninety-four patients treated with peritoneal dialysis were included in the study. Patients' age, sex, weight, waist circumference, arterial blood pressure, fasting blood glucose, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglycerides, C-reactive protein, albumin, uric acid and fibrinogen levels were recorded. Fourty-one patients diagnosed with metabolic syndrome and 53 peritoneal dialysis patients without metabolic syndrome were identified. In the metabolic syndrome group mean decrease in serum albumin and mean increase in C-reactive protein and fibrinogen was significantly different from the other group (p < 0.05, p < 0.05, p < 0.05, respectively). When gender, diabetes mellitus, and hypertension status were evaluated, the difference was not significant (p > 0.05). Peritoneal dialysis patients with metabolic syndrome should also be assessed for inflammation.